Real-Time PCR Kits
Atypical CAP Panel

Community-acquired pneumonia (CAP) is one of the leading causes of morbidity and mortality worldwide. CAP refers to any of the pneumonia or various lung diseases that occur during daily life in the community, outside the health system, in other words, in a previously healthy person who was not hospitalized before the onset of the complaint. The main bacteria causing respiratory infections including CAP are Chlamydophila pneumoniae, Mycoplasma pneumoniae and Legionella pneumophila.
Pneumonia caused by Chlamydophila pneumoniae is a worldwide occurring health problem. C. pneumoniae may colonize in the patient’s nose, throat, windpipe and lungs. Bacteria is transmitted through hand contact, inhalation and environmental surfaces which are generated when infected person who sneeze, sick coughs in close contact with a healthy individual.
Legionella pneumophila is the causative agent of legionellosis, which refers to Legionnaires’ disease and Pontiac fever. Legionnaires’ disease is a bronchopneumonia with an attack rate of 2-7% and a 2-10 days long incubation period. M. pneumoniae infections with an incubation period of 2-3 weeks, occur worldwide without relation to season or geography and can cause epidemics every 4-8 years.
Features
Workflow

Technical Specifications
Thermal Protocol | Single thermal protocol for all parameters |
Sample Types | Respiratory swabs, sputum, and bronchoalveolar lavage (BAL) |
Shelf Life | 18 Months |
Channels | FAM, HEX, Texas RED, Cy5 |
Shipping / Storage | (-90°C)-( -20°C) / -20°C |
Atypical CAP Panel Kits
Bosphore Atypical CAP Panel Kit is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the MOMP gene of Chlamydophila pneumoniae, MIP gene of Legionella pneumophila/longbeachae, P1 Adhesin gene of Mycoplasma pneumoniae from the following human biological samples; throat swab, nasopharyngeal swab, nasopharyngeal aspirate, tracheal aspirate, bronchoalveolar lavage, sputum, biopsy (tissue) samples, and culture samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Chlamydophila pneumoniae DNA is amplified and fluorescence detection is performed using the FAM filter, Legionella pneumophila/longbeachae DNAs are amplified and fluorescence detection is performed using the Texas RED filter and Mycoplasma pneumoniae DNA is amplified and fluorescence detection is performed using the Cy5 filter.
Component | FAM | HEX | Texas RED | Cy5 |
PCR Master Mix | Chlamydophila pneumoniae | Internal Control | Legionella pneumophila/longbeachae | Mycoplasma pneumoniae |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or the PCR step.