Developed by Frederick Sanger and colleagues in 1977, Sanger sequencing is a DNA sequencing method based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication.
Our range of sequencing kits developed for Sanger sequencing allows you to reach reliable and easily interpretable results in HBV, HCV and HIV drug resistance analysis.
The sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Sample Types | Human serum and plasma samples |
| Shelf Life | 18 Months |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Bosphore HBV Drug Resistance Mutation Sequencing Kit v1, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HBV Drug Resistance Mutation Sequencing Kit v1 analyzes the drug resistance mutations in the polymerase gene RT domain of the HBV DNA in human serum or plasma, encompassing all the HBV genotypes.
Bosphore HBV Drug Resistance Mutation Sequencing Kit v1 includes HBV genotyping, analysis of RT domain mutations, SHB protein mutations and SHB protein Escape mutations and analysis of drug resistance mutations indicated below: Lamivudine, Zeffix, Adefovir, Hepsera, Entecavir, Baraclude, Tenofovir DF, Telbivudine, Tyzeka, Sebivo.
We recommend our customers to sequence HBV samples above 500 IU/ml viral load.
Bosphore HCV Drug Resistance Mutation Sequencing Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore HCV Drug Resistance Mutation Sequencing Kit v1 analyzes the drug resistance mutations in the NS3 region of the hepatitis C virus RNA in human serum or plasma, in HCV genotypes 1a and 1b, and enables to analyze the drug resistance mutations indicated below: Boceprevir, Telaprevir, Simetrevir, Faldaprevir.
We recommend users to sequence only HCV genotype 1a or 1b samples with a viral load above 3000 IU/ml.
Bosphore HIV-1 Drug Resistance Mutation Sequencing Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore HIV-1 Drug Resistance Mutation Sequencing Kit v1 analyzes the drug resistance mutations in the RT, protease and integrase regions of the human immunodeficiency virus 1 RNA in human serum or plasma, encompassing all the HIV-1 genotypes and enables to analyze the drug resistance mutations indicated below: For protease region; atazanavir/r, darunavir/r, fosamprenavir/r, indinavir/r, lopinavir/r, nelfinavir, saquinavir/r, tipranavir/r. For RT region; lamivudine, abacavir, zidovudine, stavudine, didanosine, emtricitabine, tenofovir, efavirenz, etravirine, nevirapine, rilpivirine. For integrase; raltegravir and elvitegravir.
We recommend users to sequence HIV samples with a viral load above 1000 IU/ml.
Bosphore HIV-1 Drug Resistance Mutation Sequencing Kit v2, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore HIV-1 Drug Resistance Mutation Sequencing Kit v2 analyzes the drug resistance mutations in the RT and protease regions of the human immunodeficiency virus 1 RNA in human serum or plasma, encompassing all HIV-1 genotypes and enables to analyze the drug resistance mutations indicated below: For protease region; atazanavir/r, darunavir/r, fosamprenavir/r, indinavir/r, lopinavir/r, nelfinavir, saquinavir/r, tipranavir/r. For RT region; lamivudine, abacavir, zidovudine, stavudine, didanosine, emtricitabine, tenofovir, efavirenz, etravirine, nevirapine, rilpivirine.
We recommend users to sequence HIV samples with a viral load above 1000 IU/ml.
Bosphore NS5A-1a Sequencing Kit v1 is a sanger sequencing based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore NS5A-1a Sequencing Kit v1 analyzes the drug resistance mutations in the NS5A region of the hepatitis C virus RNA in human serum or plasma in HCV genotype 1a and enables to analyze the drug resistance mutations indicated in the following drug; Daclatasvir.
We recommend users to sequence only HCV genotype 1a samples with a viral load above 3000 IU/ml.
Bosphore NS5A-1b Sequencing Kit v1 is a sanger sequencing based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore NS5A-1b Sequencing Kit v1 analyzes the drug resistance mutations in the NS5A region of the hepatitis C virus RNA in human serum or plasma in HCV genotype 1b and enables to analyze the drug resistance mutations indicated in the following drug; daclatasvir.
We recommend users to sequence only HCV genotype 1b samples with a viral load above 3000 IU/ml.
Bosphore NS5B-1a Sequencing Kit v1 analyzes the drug resistance mutations in the NS5B region of the hepatitis C virus RNA in human serum or plasma in HCV genotype 1a and enables to analyze the drug resistance mutations indicated below: Sofosbuvir, Lomibuvir, Filibuvir, Benzimidazole derivates.
We recommend users to sequence only HCV genotype 1a samples with a viral load above 3000 IU/ml.
Bosphore NS5B-1b Sequencing Kit v1 analyzes the drug resistance mutations in the NS5B region of the hepatitis C virus RNA in human serum or plasma in HCV genotype 1b and enables to analyze the drug resistance mutations indicated below: Sofosbuvir, Lomibuvir, Filibuvir, Benzimidazole derivates.
We recommend users to sequence only HCV genotype 1b samples with a viral load above 3000 IU/ml.
