Real-Time PCR Kits
Hepatitis Kits
Hepatitis is inflammation of the liver tissue. If hepatitis resolves within six months, it is called acute, if it lasts longer than six months, it is chronic. Acute hepatitis may resolve on its own, progress to chronic hepatitis, or (rarely) result in acute liver failure. Chronic hepatitis may progress to liver scarring (cirrhosis), liver failure, and liver cancer.
Viral Hepatitis presents global health challenges, with death rates beginning to outnumber those from HIV/AIDS. There are 5 different and most common types of Hepatitis A, B, C, D, and E. According to the World Health Organization (WHO) 2024 Global Hepatitis Report numbers, the number of lives lost due to viral hepatitis has increased to 1.3 million of these 83% were caused by Hepatitis B (caused by Hepatitis B virus (HBV)) and 17% by Hepatitis C (caused by Hepatitis C virus (HCV)).
Anatolia Geneworks’ Bosphore Hepatitis kits provide in vitro diagnostic tools for detection, screening, quantification, and genotyping against HBV and HCV.
Features
Workflow
Technical Specifications
Thermal Protocol | Single thermal protocol for all parameters |
Sample Types | Serum and plasma |
Shelf Life | 18 Months |
Channels | FAM, HEX, Cy5 |
Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Hepatitis Kits
Bosphore HBV Detection Kit v1 detects hepatitis B virus DNA in human serum or plasma, encompassing all the major HBV genotypes (A-H). A region within the S gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter depending on the Real-Time PCR system used. The internal control can be added either during DNA extraction or PCR step.
Bosphore HBV Quantification Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HBV Quantification Kit detects and quantitates hepatitis B virus DNA in human serum or plasma, encompassing all the HBV genotypes (A-J). A region within the S gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Component | FAM | HEX |
PCR Master Mix | HBV Genotypes (A-J) |
Internal Control |
Bosphore HBV Genotype D Genotyping Kit v1 detects and characterizes the HBV genotype D in human serum or plasma. A region within the genome of HBV genotype D is detected qualitatively with the SYBR-Green Fluorescent dye by using the FAM filter.
Bosphore HCV Detection Kit v1 detects hepatitis C virus RNA in human serum and plasma, encompassing all major HCV genotypes. A region within the 5’ UTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter depending on the Real-Time PCR system used. The internal control can be added either during RNA extraction or PCR step.
Bosphore HCV Quantification Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Quantification Kit detects and quantitates hepatitis C virus RNA in human serum and plasma, encompassing all the HCV genotypes. A region within the 5’UTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition and extraction. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or PCR step.
Component | FAM | HEX |
PCR Master Mix | HCV Genotypes (1-8) | Internal Control |
Bosphore HCV Genotyping Kit v7 detects and characterizes the genotype of Hepatitis C Virus in human serum or plasma, encompassing 6 major and most predominant HCV genotypes (1,1a,1b,2,3,3a,3b,4,5,6). The analytic sensitivity is 100 IU/ml. A region within the NS5B is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An Internal Control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application errors. The amplification data of the Internal Control is detected with the Cy5 filter. The Internal Control can be added either during RNA extraction or the PCR step.
Bosphore HCV Genotyping Kit v5 detects and characterizes the NS5B region of 6 major and most predominant HCV genotypes (1, 1a, 1b, 2, 3, 4, 5, 6) from human serum or plasma samples.
Fluorescence detection is performed using FAM, HEX, and Cy5 filters. An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition, and application errors. The internal control can be added either during DNA extraction or the PCR step. Kit also contains positive controls.
Component | FAM | HEX | Cy5 |
PCR Master Mix 1 | HCV 4 | HCV 1b | Internal Control |
PCR Master Mix 2 | HCV 1a | HCV 2 | Internal Control |
PCR Master Mix 3 | – | HCV 3 | Internal Control |
PCR Master Mix 4 | HCV 1 | – | Internal Control |
PCR Master Mix 5 | HCV 5 | HCV 6 | Internal Control |
Bosphore HCV Genotype 3 Genotyping Kit v1; is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotype 3 Genotyping Kit v1 detects and characterizes the HCV genotype 3a and HCV genotype 3b in human serum or plasma. A region within the HCV Genotype 3 is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HCV Genotyping Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotyping Kit detects and characterizes the genotype of hepatitis C virus in human serumor plasma, encompassing 4 major and most predominant HCV genotypes (1,1a,1b,2,3,4). A region within the NS5B is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HCV Genotyping Kit v3, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotyping Kit detects and characterizes the genotype of hepatitis C virus in human serum or plasma, encompassing 6 major and most predominant HCV genotypes (1,1a,1b,2,3,4,5,6). A region within the NS5B is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HBV – HCV Multiplex Screening Kit v1 detects hepatitis B virus DNA or hepatitis C virus RNA in human serum and plasma, encompassing all the HBV and HCV genotypes. A region within the S gene of the HBV genome, a region within the 5’ UTR of HCV genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA/RNA extraction or PCR step.
*For research use only.
Bosphore HBV-HCV-HIV Multiplex Screening Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the S gene of HBV, 5’ UTR region of HCV, and HIV-1 LTR region of HIV from human serum and plasma samples. Fluorescence detection is performed using FAM and HEX filters. The DNA/RNA of HBV, HCV, and HIV viruses, which can be transmitted through similar routes, affect the liver and can become chronic, is detected through the FAM channel.
Component | FAM | HEX |
PCR Master Mix | HBV/HCV/HIV | Internal Control |
Caution! Attention should be paid that an increase observed in the FAM channel may indicate a positive result for one or more of the three pathogens. To determine which pathogen is responsible for the observed increase in the test result, it is recommended to use the respective Bosphore Real-Time PCR kit, which can individually detect each pathogen.
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA/RNA extraction or the PCR step.
Bosphore HDV Quantification-Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects both qualitatively and quantitatively the Hepatitis D Virus RNA in human plasma or serum, encompassing all HDV genotypes (1-8). The linear range of quantification is from 1×102 copies/ml to 1×108 copies/ml, and the analytical sensitivity is 45 copies/ml. A region within the structural antigen gene is amplified and fluorescence detection is accomplished using the FAM filter.
Component |
FAM |
HEX |
PCR Master Mix |
HDV |
Internal Control |
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or the RT-PCR step.