Real-Time PCR Kits
Hepatitis Kits
Hepatitis B virus (HBV) infection is a worldwide health problem with the highest burden of disease in Asia, Pacific Islands and Sub-Saharan Africa. There are 2 billion people infected worldwide (one third of world’s population), and 400 million suffering from chronic HBV infection (90% of infants and up to 50% of young children infected with hepatitis B will develop chronic infections). HBV infections result in roughly 1 million deaths per year caused by HBV and its complications (HBV-related liver diseases).
It is estimated that HCV has a worldwide prevalence of 3% affecting around 180 million people with between 3 to 4 million new infections each year. The vast majority of infected people (70-90%) develop chronic infection. Though chronic infection may be asymptomatic, it is a leading cause of chronic liver diseases, including cirrhosis in between 20 to 50% of patients. Treatments may be effective in 10-50% of patients depending on the applied therapy.
Hepatitis delta virus (HDV) has an epidemiological distribution similar to HBV. It has been found worldwide with unequal distribution rates. There are around 10 million people infected with HDV around the world. It has mostly similar distribution patterns of HBV infection, but with different rates. There is a highest incidence HDV infection in Southern Italy, the Mediterranean region and in some parts of Africa and Asia. There is an average incidence of HDV infection in Turkey. 10% of the patients with HBV in the west and 15-25% of patients in the central and eastern regions have positive for anti-HDV.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Typing | Serum and plasma |
| Shelf Life | 18 Months |
| Channels | FAM, HEX/VIC, Cy5 |
| Shipping / Storage | -80°C / -20°C |
Hepatitis Kits
Bosphore HCV Genotyping Kit v5 detects and characterizes the NS5B region of 6 major and most predominant HCV genotypes (1, 1a, 1b, 2, 3, 4, 5, 6) from human serum or plasma samples.
Fluorescence detection is performed using FAM, HEX, and Cy5 filters. An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition, and application errors. The internal control can be added either during DNA extraction or the PCR step. Kit also contains positive controls.
Bosphore HCV Detection Kit v1 detects hepatitis C virus RNA in human serum and plasma, encompassing all major HCV genotypes. A region within the 5’ UTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter depending on the Real-Time PCR system used. The internal control can be added either during RNA extraction or PCR step.
Bosphore HBV Detection Kit v1 detects hepatitis B virus DNA in human serum or plasma, encompassing all the major HBV genotypes (A-H). A region within the S gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter depending on the Real-Time PCR system used. The internal control can be added either during DNA extraction or PCR step.
Bosphore HCV Quantification Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Quantification Kit detects and quantitates hepatitis C virus RNA in human serum and plasma, encompassing all the HCV genotypes. A region within the 5’UTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition and extraction. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore Ultra HCV Quantitation / Detection Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. HCV Quantification Kit detects and quantitates hepatitis C virus RNA in human serum and plasma, encompassing all the HCV genotypes. A region within the 5’UTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition and DNA extraction. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Ultra HBV Quantitation / Detection Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Ultra HBV Quantitation / Detection Kit detects and quantitates hepatitis B virus DNA in human serum or plasma, encompassing all the HBV genotypes (A-H). A region within the S gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition and DNA extraction. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore HDV Quantification / Detection Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HDV Quantification / Detection Kit v1 detects both qualitatively and quantitatively the hepatitis D virus RNA in human plasma or serum, encompassing all major HDV genotypes. A region within the structural antigen gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction problems and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either in the extraction or during RT-PCR step.
Bosphore HBV Quantification Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HBV Quantification Kit detects and quantitates hepatitis B virus DNA in human serum or plasma, encompassing all the HBV genotypes (A-H). A region within the S gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore HBV – HCV Multiplex Screening Kit v1 detects hepatitis B virus DNA or hepatitis C virus RNA in human serum and plasma, encompassing all the HBV and HCV genotypes. A region within the S gene of the HBV genome, a region within the 5’ UTR of HCV genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA/RNA extraction or PCR step.
*For research use only.
Bosphore HBV-HCV-HIV Multiplex Screening Kit v1 detects hepatitis B virus DNA, hepatitis C virus RNA or human immunodeficiency virus 1 RNA in human serum and plasma, encompassing all the HBV, HCV and HIV-1 genotypes. A region within the S gene of the HBV genome, a region within the 5’ UTR of HCV genome, a region within the HIV-1 LTR is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX/Cy5/JOE filter. The internal control can be added either during extraction or PCR step.
*For research use only.
Bosphore HCV Genotype 3 Genotyping Kit v1; is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotype 3 Genotyping Kit v1 detects and characterizes the HCV genotype 3a and HCV genotype 3b in human serum or plasma. A region within the HCV Genotype 3 is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HCV Genotyping Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotyping Kit detects and characterizes the genotype of hepatitis C virus in human serumor plasma, encompassing 4 major and most predominant HCV genotypes (1,1a,1b,2,3,4). A region within the NS5B is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HCV Genotyping Kit v3, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HCV Genotyping Kit detects and characterizes the genotype of hepatitis C virus in human serum or plasma, encompassing 6 major and most predominant HCV genotypes (1,1a,1b,2,3,4,5,6). A region within the NS5B is amplified and fluorescence detection is accomplished using the FAM and HEX filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or PCR step.
Bosphore HBV Genotype D Genotyping Kit v1 detects and characterizes the HBV genotype D in human serum or plasma. A region within the genome of HBV genotype D is detected qualitatively with the SYBR-Green Fluorescent dye by using the FAM filter.
