Real-Time PCR Kits
HLA-B
Human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex proteins in humans. These cell-surface proteins are responsible for the regulation of the immune system.
HLAB27 is crucial for diagnosis of ankylosing spondylitis (AS) and AS related spondylarthropathy. HLAB27 takes an important role in disease pathogenesis and there are several types of the disease. The prevalence of the disease is altering between different ethnic groups.
HLA-B 57:01 is a genetic variation linked to hypersensitivity to the antiretroviral (ARV) drug Abacavir. Combination ARV therapy (cART) is an effective treatment strategy for HIV infections and it decreases morbidity and mortality rates for HIV-infected patients. Abacavir (ABC) is a guanosine analog and it is a reverse transcriptase inhibitor. The drug has been approved by US Food and Drug Administration (FDA) since 1998 and it is used in combination with other ARV drugs.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Whole blood and blood culture |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Cy5 |
| Shipping / Storage | –90, -20°C / -20°C |
HLA-B Detection Kits
Bosphore HLAB27 Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HLAB27 Detection Kit v1 detects HLAB27 allele existence in human biological samples. HLAB27 allele is amplified and fluorescence detection is accomplished using the FAM filter. For the situations that HLAB27 allele is absent in the sample, a housekeeping gene GAPDH is also amplified as internal control. Fluorescence detection of GAPDH is accomplished using the Cy5 filter in order to prove that PCR reaction is performed accurately.
Bosphore HLAB27 Detection Kit v2 detects HLAB27 allele existence in human biological samples. HLAB27 allele is amplified and fluorescence detection is accomplished using the FAM filter. For the situations that HLAB27 allele is absent in the sample, a housekeeping gene GAPDH is also amplified as internal control. Fluorescence detection of GAPDH is accomplished using the Cy5 filter in order to prove that PCR reaction is performed accurately.
Only a single incubation with the EX-Tract DNA solution is enough to prepare the samples for qPCR. There is no need to apply an external DNA isolation.
Bosphore HLA-B57:01 Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore HLA-B57:01 Detection Kit v1 detects HLA-B*57:01 allele existence in exon 2 and exon 3 in human biological samples.
In the 1st well, HLA-B*57:01 allele in exon 2 is amplified and fluorescence detection is accomplished using the FAM filter.
In the 2nd well, HLA-B*57:01 allele in exon3 is amplified and fluorescence detection is accomplished using the HEX filter.
For the situations that HLA-B*57:01 allele is absent in the sample, a housekeeping gene GAPDH is also amplified as internal control in both wells. Fluorescence detection of GAPDH is accomplished using the Cy5 filter in order to prove that PCR reaction is performed accurately.
