Real-Time PCR Kits
Meat Species Identification
Arguably most important difficulties that the meat and dairy industry is experiencing today, are detecting adulteration in food products and ingredient origin determination due to the strict regulatory environment. Intentional or unintentional, meat adulteration and origin falsification damage public and animal health, brand value, consumer trust and raise ethical concerns. Although differentiating close relative species from a complex sample is challenging in most other methods, Real-Time PCR method; with its sensitivity, specificity and fast application, is considered as the golden standard for meeting these challenges.
Our Bosphore Meat Species Identification kits offer sensitive, accurate and reliable results in an easy to use, customizable and time-efficient workflow.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Processed and unprocessed food, feed, seed samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Meat Species Identification Kits
Bosphore Vegan Detection Kit v1 is a Real-Time PCR kit that detects animal mitochondrial DNA (mtDNA) belonging to classes such as mammals, birds, fish, insects, spiders, and molluscs from samples such as meat and meat products, milk and dairy products, food with or without animal products, food additives, feed, and feed additives. Fluorescence detection is performed using FAM and HEX filters.
The kit includes an internal control to check PCR inhibition and application errors. The internal control is added to the PCR reaction mixture. Fluorescence detection for the internal control is performed using a HEX filter.
Since the kit also detects human samples, it is important that the user wears gloves and a mask during application to prevent false positive results.
Bosphore Species Identification Kit Pork v1 detects pork DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the pork mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Fish v1 detects fish DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the fish mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Turkey v1 detects turkey DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the turkey mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Turkey v3 is a research use only Real-Time PCR kit that detects and differentiates turkey mtDNA from meat and meat product samples. Fluorescence detection is performed using FAM and Cy5 filters. Turkey mtDNA is amplified and fluorescence detection is performed using the FAM filter.
The kit includes an internal control (IC) to control PCR inhibition and application errors. The internal control can be added to the PCR reaction mixture. Fluorescence detection for the internal control is performed using a Cy5 filter.
The LOD (Limit of Detection) of Bosphore Species Identification Kit Turkey v3 is defined as 1%.
For Research Use Only
Bosphore Species Identification Kit Chicken v1 detects chicken DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the chicken mitochondrial genome is amplified and fluorescence detection is accomplished using the CY5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Chicken v3 is a research use only Real-Time PCR kit that detects and differentiates Chicken mtDNA from heat-treated and non-heat-treated meat and meat products. Fluorescence detection is performed using HEX and Cy5 filters. Chicken mtDNA is amplified and fluorescence detection is performed using the Cy5 filter.
The kit includes an internal control (IC) for PCR inhibition and application errors. The internal control can be added to the PCR reaction mixture. Fluorescence detection for the internal control is performed using the HEX filter.
The LOD (Limit of Detection) of Bosphore Species Identification Kit Chicken v3 is defined as 1%.
For Research Use Only
Bosphore Species Identification Kit Donkey v1 detects donkey DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the donkey mitochondrial genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Dog v1 is a Real-Time PCR kit for research use that detects and differentiates dog-specific mitochondrial DNA from samples such as heat-treated and non-heat-treated meat and meat products, food containing animal products, food additives, feed, and feed additives. Fluorescence detection is performed using HEX and Cy5 filters. Dog mitochondrial DNA is amplified, and fluorescence detection is performed using Cy5 filter.
| Component | HEX | Cy5 |
| PCR Master Mix | Internal Control | Dog Mitochondrial DNA |
The kit includes Internal Control (IC) to check PCR inhibition and application errors. Internal Control can be added to the PCR reaction mix. Fluorescence detection for Internal Control is performed using a HEX filter.
Bosphore Species Identification Kit Horse v1 detects horse DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the horse mitochondrial genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Sheep v1 detects sheep DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the sheep mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Goat v1 detects goat DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the goat mitochondrial genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Primate v1 detects Homininae subfamily including human (H. sapiens), gorilla (Gorilla gorilla), chimpanzee (P. troglodytes), bonobo (P. paniscus), in various biological samples such as cosmetics, food or forensic samples. A region within the major histocompatibility complex (MHC) gene is amplified and fluorescence detection is accomplished using the FAM filter. An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added in the PCR step.
Bosphore Species Identification Kit Water Buffalo (B. bubalis) v1 With Real detects the presence of buffalo DNA in samples containing heat-treated and untreated meat and meat products, milk and dairy products, food containing animal products, food additives, feed, feed additives.
Internal Control DNA is detected using the HEX filter, and Buffalo DNA is detected through the Cy5 filter.
Bosphore Species Identification Kit Bovine v1 detects bovine DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the bovine mitochondrial genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Bovine Gender Quantification Kit v1 detects and quantifies the gender of bovine (Bos taurus) from unprocessed meat by using Real-Time PCR method. X chromosome detection is accomplished using the HEX filter, and the Y chromosome is detected using the FAM filter.
Bosphore Species Identification Kit Turkey-Chicken v1 detects turkey and chicken DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the turkey genome is amplified and fluorescence detection is accomplished using FAM filter. A region within the chicken genome is amplified and fluorescence detection is accomplished using Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Turkey-Chicken v3 is a Real-Time PCR kit that detects and differentiates turkey and chicken mitochondrial DNA (mtDNA) in heat-treated and non-heat-treated meat and meat product samples. The sensitivity/detection limit of the Bosphore Species Identification Kit Turkey-Chicken v3 kit is 1%. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. Turkey mtDNA is amplified, and fluorescence detection is performed using FAM filter. Chicken mtDNA is amplified, and fluorescence detection is performed using Cy5 filter.
| Component | FAM | HEX | Cy5 |
| PCR Master Mix | Turkey mtDNA | Internal Control | Chicken mtDNA |
The kit contains Internal Control (IC) to check PCR inhibition and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using HEX filter.
Bosphore Species Identification Bovine-Pork Kit v1 (Halal Kit) detects bovine and pork DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the bovine mitochondrial genome is amplified and fluorescence detection is accomplished using the HEX filter. A region within the pork mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter. An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Goat-Sheep v1 detects goat and sheep DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the goat mitochondrial genome is amplified and fluorescence detection is accomplished using the HEX filter. A region within the sheep mitochondrial genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Species Identification Kit Horse-Donkey v1 detects horse and donkey DNA in meat, skin, tendon, bone, fat, gelatine, candy and dairy samples. A region within the horse genome is amplified and fluorescence detection is accomplished using HEX filter. A region within the donkey genome is amplified and fluorescence detection is accomplished using Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the FAM filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Halal (Horse-Pork-Donkey) Kit v2 is a Real-Time PCR kit for research use that detects and differentiates Pig, Horse, and Donkey mitochondrial DNA (mtDNA) in heat-treated and non-heat-treated meat and meat product samples. Internal Control DNA is detected using Texas Red filter, while pig, horse, and donkey DNA is detected using the FAM, HEX, and Cy5 filters, respectively. Fluorescence detection is performed using FAM, HEX, Texas Red, and Cy5 filters. Pig mtDNA is amplified, and fluorescence detection is performed using FAM filter. Horse mtDNA is amplified and fluorescence detection is performed using the HEX filter. Donkey mtDNA is amplified, and fluorescence detection is performed using the Cy5 filter.
| Component | FAM | HEX | Texas Red | Cy5 |
| PCR Master Mix | Pig mtDNA | Horse mtDNA | Internal Control | Donkey mtDNA |
The kit contains Internal Control (IC) to check PCR inhibition and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using Texas Red filter.
