Real-Time PCR Kits
Herpes Kits
Herpesviridae is a large family of viruses causeing infections and certain diseases in humans and animals. Members of this family are also known as herpesviruses. Herpesvirus was established as a a genus with 23 viruses among four groups by International Committee on the Taxonomy of Viruses (ICTV). Nine herpesvirus types are known to infect humans:
Herpes simplex viruses 1 and 2 (HSV-1 and HSV-2, also known as HHV-1 and HHV-2), varicella-zoster virus (VZV, also called HHV-3), Epstein–Barr virus (EBV or HHV-4), human cytomegalovirus (HCMV or HHV-5), human herpesvirus 6A and 6B (HHV-6A and HHV-6B), human herpesvirus 7 (HHV-7), and HHV-8.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Typing | Serum and plasma |
| Shelf Life | 18 Months |
| Channels | FAM, HEX/VIC, Cy5 |
| Shipping / Storage | -80°C / -20°C |
Herpes Kits
Bosphore® CMV Quantification Kit, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® CMV Quantification Kit detects and quantitates Cytomegalovirus DNA in human biological samples such as serum, plasma, urine, encompassing all major CMV genotypes. A region within the DNA polymerase gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore CMV Quantification-BKV Detection Kit v1 detects and quantitates Cytomegalovirus (CMV) DNA encompassing all CMV genotypes and detects four main genotypes of BK Virus (BKV) DNA in human biological samples including serum, plasma, urine, biopsy specimen, amniotic fluid, CSF, tissue samples.. A specific region within CMV genome is amplified and the detection is accomplished using FAM filter. A specific region within BKV genome is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. Amplification data of internal control is detected with Cy5 filter. Internal control can be added either in extraction or PCR step.
Bosphore® EBV Quantification Kit, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® EBV Quantification Kit v1 detects and quantitates Epstein-Barr Virus (Human Herpes Virus-4/HHV-4) DNA in human biological samples such as serum, plasma, urine or tissue, encompassing all EBV subtypes. An amplicon within the IR1 region of EBV genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5/HEX/JOE filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore® HHV-6 Detection Kit, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® HHV-6 Detection Kit v1 detects Human Herpesvirus (HHV-6 DNA) in human serum or plasma, encompassing all the major HHV-6 subtypes. A region within the U57 gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX/JOE filter. The internal control can be added during PCR step.
Bosphore® HHV-6 Quantification Kit v1 is, a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® HHV-6 Quantification Kit v1 detects and quantitates Human Herpesvirus (HHV-6 DNA) in human serum, plasma, whole blood and CSF samples, encompassing all the major HHV-6 subtypes. A region within the U57 gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX/JOE filter. The internal control is added either in extraction or PCR step.
Bosphore® HHV-7 Detection Kit v1 detects Human Herpesvirus (HHV-7 DNA) in human serum or plasma, encompassing all the major HHV-7 subtypes. The analytic sensitivity is 185 copies/ml. A region gene is amplified and fluorescence detection is accomplished using the CY5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added during PCR step.
Bosphore® HHV-8 Quantification Kit v1 detects and quantitates Human Herpesvirus 8 (HHV-8) DNA in human biological samples such as serum and plasma, encompassing all the major HHV-8 subtypes. The analytic sensitivity is 0.85 copies/µl. A region within the HHV-8 is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added either in DNA extraction or PCR step.
Bosphore® HSV 1-2 Genotyping Kit v1, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® HSV 1-2 Genotyping Kit v1 detects and distinguishes Herpes Simplex Virus 1 and 2 in human biological samples. A region within the Glycoprotein D gene within the HSV1 genome is amplified and fluorescence detection is accomplished using the FAM filter. A region within the Glycoprotein D gene within the HSV2 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore® Vesicular Rash Panel Kit is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Vesicular Rash Panel Kit detects and characterizes Herpes simplex virus 1, Herpes simplex virus 2 and Varicella-Zoster virus DNA in human biological samples. Fluorescence detection is accomplished by using FAM, HEX, Texas RED and Cy5 filters. Herpes simplex virus 1 is amplified and fluorescence detection is accomplished using the FAM filter, Herpes Simplex Virus 2 is amplified and fluorescence detection is accomplished using the Cy5 filter and Varicella-Zoster Virus is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore VZV Detection Kit v1, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® VZV Detection Kit v1 detects Varicella zoster virus (also known as Human Herpes Virus 3; VZV DNA) in human serum or plasma, encompassing all the major VZV subtypes. A region within the ORF 38 gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX/JOE filter. The internal control can be added during PCR step.
