Real-Time PCR Kits
Viral Infections in the Immunosuppressed Panel
Immunosuppression is a decrease in the immune system’s activity or effectiveness. Immunosuppression can happen as a negative reaction to the treatment of other illnesses or because of the immunosuppressive effects of some immune system components on other immune system components. Some people who have a weakened immune system and who are immunocompromised are more likely to get sick for a longer period.
Adenovirus (AdV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) are major causes of morbidity and mortality in immunosuppressed individuals. After primary infection, these viruses may remain latent and then occur through immunosuppression. Infection is common with seroprevalence rates increasing steadily from 65% among 40- to 49-year-olds to 91% in those aged 80 years or older.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Whole blood, serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-( -20°C) / -20°C |
Viral Infections in the Immunosuppressed Panel Kits
Bosphore Viral Infections in the Immunosuppressed Panel Kit is an in vitro diagnostic Real-Time Polymerase Chain Reaction (qPCR) kit intended for qualitative detection of Adenovirus, EBV (Epstein-Barr Virus), and CMV (Cytomegalovirus) viral DNA extracted from human serum, plasma, bone marrow, amniotic fluid, urine, cerebrospinal fluid, bronchoalveolar lavage, whole blood and eye swab in individuals suspected of being infected with related pathogens by health authorities.
Bosphore Viral Infections in the Immunosuppressed Panel Kit detects three pathogens; Adenovirus, EBV (Epstein-Barr Virus), and CMV (Cytomegalovirus) in a single tube: the conserved regions of DNA polymerase gene of CMV, Hexon gene of Adenovirus, and IR1 region of Epstein-Barr virus are used as target sites to discriminate pathogens specifically. Fluorescence detection is accomplished using FAM, HEX, Texas RED, and Cy5 filters.
An internal control is included in the kit to control DNA extraction, PCR inhibition, and application mistakes. The internal control is a synthetic DNA molecule.
|
FAM |
HEX |
Texas RED |
Cy5 |
|
Cytomegalovirus |
Epstein-Barr Virus |
Internal Control |
Adenovirus |
Bosphore Viral Infections in the Immunosuppressed Maxi Panel Bundle Kit detects and characterizes JCV DNA, BKV DNA, CMV DNA, EBV DNA and adenovirus DNA human biological samples; serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Texas Red | Cy5 | |
| PCR Master Mix 1
Box-1 |
JCV | BKV | – | Internal Control |
| PCR Master Mix 1
Box-2 |
CMV | EBV | Internal Control | Adenovirus |
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application errors. Amplification data of the internal control is detected with Cy5 or Texas RED filter as indicated in the table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
Bosphore VIP Quantification Bundle Kit has been designed to detect, discriminate and quantitate cytomegalovirus (CMV), Epstein-Barr virus (EBV/human herpes virus-4/HHV-4) and adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage.
Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in two PCR tubes.
| FAM | HEX | Cy5 | |
| PCR Master Mix 1
Box-1 |
CMV | Internal Control | – |
| PCR Master Mix 1
Box-2 |
Adenovirus (ADV) | Epstein-Barr virus (EBV) | Internal Control |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.
Bosphore EBV-Adenovirus Quantification Kit has been designed to detect, discriminate and quantitate Epstein-Barr virus (EBV/human herpes virus-4/HHV-4) and Adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage.
Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in one PCR tube.
| FAM | HEX | Cy5 |
| Adenovirus (ADV) | Epstein-Barr virus (EBV) | Internal Control |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors. The internal control can be added either during DNA extraction or PCR step.
Bosphore JCV-BKV Detection Kit v2 detects and characterizes JC Virus and BK virus in human biological samples. The analytic sensitivity is 175 copies/ml for JCV and 75 copies/ml for BKV. A region within JCV genome is amplified and fluorescence detection is accomplished using the FAM filter. A region within BKV genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added either in extraction or PCR step.
