Real-Time PCR Kits
Tropical Fever Panel
Infections that are common in or unique to tropical and subtropical regions are collectively identified as tropical fevers. Some of the common tropical fevers are Dengue, Malaria, Scrub Typhus, Leptospirosis, Typhoid fever, Chikungunya, West Nile, and S. enterica infections.
These infections often present as undifferentiated fevers with organ failures. Several viruses, bacteria, and protozoa cause tropical fever, and they are often transmitted by insect bites such as mosquitos. Delayed specific treatment may lead to increased morbidity and mortality rates including a significant number of patients with one or more organ failures requiring intensive care. However, clinical diagnosis might be challenging due to undifferentiated fever and overlapping symptoms. Similarly, laboratory confirmation by serological techniques may not be available or reliable in the first few days. This is why molecular diagnostic tools have become crucial in cases of Tropical Fever since they are faster and more reliable.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Whole blood and plasma |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Tropical Fever Panel Kits
Bosphore Tropical Fever Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Plasmodium falciparum, Plasmodium malariae, Salmonella enterica, Plasmodium vivax, Dengue virus, Chikungunya virus, West Nile virus, Leptospira spp., and Plasmodium ovale DNA and RNA in human biological samples including whole blood and plasma. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filter. The internal control can be added either during DNA/RNA extraction or the PCR step.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED (Gene) | Cy5
(Gene) |
| PCR Master Mix 1 | Plasmodium falciparum
(18S ribosomal RNA) |
Plasmodium malariae
(18S ribosomal RNA) |
Internal Control | Salmonella enterica
(TTR site) |
| PCR Master Mix 2 | Plasmodium vivax
(18S ribosomal RNA) |
Dengue virus
(3’UTR) |
Internal Control | Chikungunya virus
(Envelope protein gene) |
| PCR Master Mix 3 | West Nile virus
(3’UTR) |
Plasmodium ovale
(18S ribosomal RNA) |
Internal Control | Leptospira spp.
(lipl32 gene) |
Bosphore Tropical Fever Panel Kit v2 detects and characterizes Plasmodium falciparum, Plasmodium malariae, Salmonella enterica, Plasmodium vivax, Dengue virus, Chikungunya virus, West Nile virus and Plasmodium ovale DNA and RNA in human biological samples such as whole blood and plasma samples. Fluorescence detection is accomplished by using FAM, HEX, Texas RED, and Cy5 filters.
Plasmodium falciparum is amplified, and fluorescence detection is accomplished using the FAM filter, Plasmodium malariae is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica is amplified, and fluorescence detection is accomplished using the Cy5 filter in the first tube with PCR Master Mix 1.
In the Second tube, Plasmodium vivax is amplified, and fluorescence detection is accomplished using the FAM filter, Dengue virus is amplified, and fluorescence detection is accomplished using the HEX filter and Chikungunya virus is amplified and fluorescence detection is accomplished using the Cy5 filter with PCR Master Mix 2.
In the third tube, the West Nile virus is amplified, and fluorescence detection is accomplished using the FAM filter, Plasmodium ovale is amplified and fluorescence detection is accomplished using the HEX filter with PCR Master Mix 3.
An Internal Control has been integrated into the kit to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with the Texas Red filter. The Internal Control can be added either during DNA extraction or the PCR step.
