Sepsis Panel

IVD CE

Sepsis is a syndrome commonly caused by the inflammatory response of the immune system because of bacterial, viral, protozoan or fungal infections, which may result in organ failure and death if progressed. Primary infection-causing sepsis can be seen in the lungs, brain, urinary tract, skin or abdominal region. Sepsis can lead to severe sepsis, septic shock stages and death due to dysfunction of one or more organs if early diagnosis and treatment is not provided. It is known as the main cause of maternal and neonatal diseases and deaths, especially in low- and middle-income countries.

The organisms that cause sepsis can be exampled as E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. Risk factors for the development of sepsis include early and advanced age and weakened immune system due to cancer, diabetes, major trauma or burns.

Features

Single Tube Master Mix

Internal Control for Each Reaction

Package Size Options

Compatibility with Various Systems

Expert Support

Workflow

Technical Specifications

Thermal Protocol Single thermal protocol for all parameters
Sample Types Whole blood or blood culture
Shelf Life 18 Months
Channels FAM, HEX, Texas Red, Cy5
Shipping / Storage (-90°C)-(-20°C) / -20°C

Sepsis Panel Kits

Bosphore Sepsis Pathogens Panel Kit v1 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

In the first tube with PCR Master Mix 1; Escherichia coli is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecalis is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the second tube with PCR Master Mix 2; Klebsiella pneumoniae is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus spp. is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecium is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the third tube with PCR Master Mix 3; Acinetobacter baumannii virus is amplified and fluorescence detection is accomplished using the FAM filter, Stenotrophomonas maltophilia is amplified and fluorescence detection is accomplished using the HEX filter and Pseudomonas aeruginosa is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the fourth tube with PCR Master Mix 4; Candida albicans is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus epidermidis is amplified and fluorescence detection is accomplished using the HEX filter and Candida glabrata is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the fifth tube with PCR Master Mix 5; Aspergillus spp. is amplified and fluorescence detection is accomplished using the FAM filter, Candida tropicalis is amplified and fluorescence detection is accomplished using the HEX filter and Streptococcus spp. is amplified and fluorescence detection is accomplished using the Cy5 filter.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Sepsis Pathogens Panel Kit v2 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium Pseudomonas aeruginosa, Haemophilus influenzae (type B), Neisseria meningitidis, Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

FAM HEX Texas Red Cy5
Tube 1 E. coli S. aureus Internal Control E. faecalis
Tube 2 K. pneumoniae Staphylococcus spp. Internal Control E. faecium
Tube 3 H. influenzae (type B) S. pneumoniae Internal Control P. aeruginosa
Tube 4 X N. meningitidis Internal Control Streptococcus spp.
Tube 5 X L. monocytogenes Internal Control S. enterica

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Sepsis Pathogens Panel Kit v3 detects and characterizes E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp., Streptococcus spp., Haemophilus influenzae (type B), Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

FAM HEX Texas Red Cy5
Tube 1 E. coli S. aureus Internal Control E. faecalis
Tube 2 K. pneumoniae Staphylococcus spp. Internal Control E. faecium
Tube 3 A. baumannii S. maltophilia Internal Control P. aeruginosa
Tube 4 C. albicans S. epidermidis Internal Control C. glabrata
Tube 5 Aspergillus spp. C. tropicalis Internal Control Streptococcus spp.
Tube 6 H. influenzae (type B) S. pneumoniae Internal Control X
Tube 7 X L. monocytogenes Internal Control S. enterica

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

The Bosphore Sepsis Panel Bundle Kit consists of a combination of Bosphore Sepsis Screening Kit (Box 1), and Bosphore ABR Screening Kit (Box 2).

The Bosphore Sepsis Screening Kit is a Real-Time PCR kit designed to detect and characterize Streptococcus agalactiae, Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis, Klebsiella pneumoniae, Staphylococcus spp., Serratia marcescens, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Candida krusei, Pseudomonas aeruginosa, Candida albicans, Staphylococcus epidermidis, Enterobacter cloacae, Candida glabrata, Aspergillus spp., Candida tropicalis, Escherichia coli, Streptococcus spp., Haemophilus influenzae (type B), Listeria monocytogenes, Candida parapsilosis, Salmonella enterica, Klebsiella oxytoca, Proteus mirabilis, Enterobacter (klebsiella) aerogenes, Neisseria meningitidis, Fusarium spp., Campylobacter spp., Candida auris, Streptococcus pyogenes in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

The Bosphore ABR Screening Kit is a Real-Time PCR kit designed to detect and characterize resistance genes for New Delhi Metallo-beta-lactamase (NDM), Oxacillinase-48-like (OXA 48), K. pneumoniae carbapenemase (KPC), Imipenemase (IMP), Verona Integron-Enconded Metallo-beta-lactamase (VIM), Cefotaximase (CTX-M), Mobilized colistin resistance-1 (MCR-1), Vancomycin resistance A (Van A), Vancomycin resistance B (Van B), Methicillin resistance gene (mecA) in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Component FAM HEX Texas RED Cy5
Box 1 PCR Master Mix 1 S. agalactiae

(cfB gene)

 S. aureus

(nucA gene)

 S. pneumoniae

(lytA gene)

 E. faecalis

(DDL gene)
PCR Master Mix 2 K. pneumoniae

(Hemolysin gene)

 Staphylococcus  spp.

(tuf gene)

 S. marcescens

(16S rRNA/ITS/23S rRNA)

 E. faecium

(DDL gene)
PCR Master Mix 3 A. baumannii (T6SS gene) S. maltophilia

(ssrA gene)

 C. krusei

(ITS2 region)

 P. aeruginosa

(ecfX gene)
PCR Master Mix 4 C. albicans

(ITS2 region)

 S. epidermidis

(sodA gene)

 E. cloacae

(rpoB gene)

 C. glabrata

(ITS2 region)
PCR Master Mix 5 Aspergillus spp.

(18S rRNA gene)

 C. tropicalis

(ITS2 region)

 E. coli

(uidA gene)

 Streptococcus  spp.

(16S rRNA/

ITS/23S rRNA gene)
PCR Master Mix 6 H. influenzae (type B)

(bcs3 gene)

 L. monocytogenes

(hlyA gene)

 C. parapsilosis

(ITS2 region)

 S. enterica

(ttrA-ttrC gene)
PCR Master Mix 7 K. oxytoca

(pehX gene)

 P. mirabilis

(ureR gene)

 E. (klebsiella) aerogenes

(gyrB gene)

 N. meningitidis

(ctrA gene)
PCR Master Mix 8 Fusarium spp.

(TEF-1 gene)

 Campylobacter spp.

(16S rRNA gene)

 C. auris

(ITS1/5.8S rRNA/ITS2/ LSU rRNA gene)

 Internal Control
PCR Master Mix 9 – – S. pyogenes

(speB gene)

 Internal Control
Component FAM HEX Texas RED Cy5
Box 2 PCR Master Mix 1 NDM OXA 48 – KPC
PCR Master Mix 2 IMP VIM CTX-M MCR-1
PCR Master Mix 3 Van A Van B mecA Internal Control

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only Box 1 – PCR Master Mix 8, 9, and Box 2 – PCR Master Mix 3. The internal control can be added either during DNA extraction or the PCR step.

The Bosphore ABR Screening Kit is a Real-Time PCR kit designed to detect and characterize resistance genes for New Delhi metallo-beta-lactamase (NDM), oxacillinase-48-like (OXA 48), K. pneumoniae carbapenemase (KPC), imipenemase (IMP), verona integron-enconded metallo-beta-lactamase (VIM), cefotaximase (CTX-M), mobilized colistin resistance-1 (MCR-1), vancomycin resistance A (Van A), vancomycin resistance B (Van B), methicillin resistance gene (mecA) in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

  FAM HEX Texas RED Cy5
PCR Master Mix 1 NDM OXA 48 – KPC
PCR Master Mix 2 IMP VIM CTX-M MCR-1
PCR Master Mix 3 Van A Van B mecA Internal Control

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only PCR Master Mix 3. The internal control can be added either during DNA extraction or the PCR step.

Bosphore Sepsis Panel Bundle Kit is a Real-Time PCR kit designed to detect and characterize 32 different pathogens.

The target pathogens of the Bosphore Sepsis Screening Kit includes;

  • Gram(-) A. baumannii, Campylobacter spp, E. aerogenes, E. cloacae, E. coli, H. influenzae type b, K. oxytoca, K. pneumoniae, N. meningitidis, P. aeruginosa, P. mirabilis, S. enterica, S. maltophilia, S. marcescens,
  • Gram(+) E. faecalis, E. faecium, L. monocytogenes, Staphylococcus spp., S. aureus, S. epidermidis, Streptococcus spp., S. agalactiae, S. pneumoniae, S. pyogenes, and also
  • Fungi Aspergillus spp., C. albicans, C. glabrata, C. krusei, C. auris, C. tropicalis, C. parapsilosis, Fusarium spp.

Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Internal and positive controls were integrated into the kit.

Component FAM HEX Texas RED Cy5
PCR Master Mix 1 S. agalactiae S. aureus S. pneumoniae E. faecalis
PCR Master Mix 2 K. pneumoniae Staphylococcus spp. S. marcescens E. faecium
PCR Master Mix 3 A. baumannii S. maltophilia C. krusei P. aeruginosa
PCR Master Mix 4 C. albicans S. epidermidis E. cloacae C. glabrata
PCR Master Mix 5 Aspergillus spp. C. tropicalis E. coli Streptococcus spp.
PCR Master Mix 6 H. influenzae (type B) L. monocytogenes C. parapsilosis S. enterica
PCR Master Mix 7 K. oxytoca P. mirabilis E. (Klebsiella) aerogenes N. meningitidis
PCR Master Mix 8 Fusarium spp. Campylobacter spp. C. auris Internal Control
PCR Master Mix 9 X X S. pyogenes Internal Control

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only PCR Master Mix 8 and 9. The internal control can be added either during DNA extraction or the PCR step.

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