Real-Time PCR Kits
Sepsis Panel
Sepsis is a syndrome commonly caused by the inflammatory response of the immune system because of bacterial, viral, protozoan or fungal infections, which may result in organ failure and death if progressed. Primary infection-causing sepsis can be seen in the lungs, brain, urinary tract, skin or abdominal region. Sepsis can lead to severe sepsis, septic shock stages and death due to dysfunction of one or more organs if early diagnosis and treatment is not provided. It is known as the main cause of maternal and neonatal diseases and deaths, especially in low- and middle-income countries.
The organisms that cause sepsis can be exampled as E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. Risk factors for the development of sepsis include early and advanced age and weakened immune system due to cancer, diabetes, major trauma or burns.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Whole blood or blood culture |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Sepsis Panel Kits
Bosphore Sepsis Pathogens Panel Kit v1 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.
In the first tube with PCR Master Mix 1; Escherichia coli is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecalis is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the second tube with PCR Master Mix 2; Klebsiella pneumoniae is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus spp. is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecium is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the third tube with PCR Master Mix 3; Acinetobacter baumannii virus is amplified and fluorescence detection is accomplished using the FAM filter, Stenotrophomonas maltophilia is amplified and fluorescence detection is accomplished using the HEX filter and Pseudomonas aeruginosa is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the fourth tube with PCR Master Mix 4; Candida albicans is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus epidermidis is amplified and fluorescence detection is accomplished using the HEX filter and Candida glabrata is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the fifth tube with PCR Master Mix 5; Aspergillus spp. is amplified and fluorescence detection is accomplished using the FAM filter, Candida tropicalis is amplified and fluorescence detection is accomplished using the HEX filter and Streptococcus spp. is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Sepsis Pathogens Panel Kit v2 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium Pseudomonas aeruginosa, Haemophilus influenzae (type B), Neisseria meningitidis, Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Texas Red | Cy5 | |
| Tube 1 | E. coli | S. aureus | Internal Control | E. faecalis |
| Tube 2 | K. pneumoniae | Staphylococcus spp. | Internal Control | E. faecium |
| Tube 3 | H. influenzae (type B) | S. pneumoniae | Internal Control | P. aeruginosa |
| Tube 4 | X | N. meningitidis | Internal Control | Streptococcus spp. |
| Tube 5 | X | L. monocytogenes | Internal Control | S. enterica |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Sepsis Pathogens Panel Kit v3 detects and characterizes E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp., Streptococcus spp., Haemophilus influenzae (type B), Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Texas Red | Cy5 | |
| Tube 1 | E. coli | S. aureus | Internal Control | E. faecalis |
| Tube 2 | K. pneumoniae | Staphylococcus spp. | Internal Control | E. faecium |
| Tube 3 | A. baumannii | S. maltophilia | Internal Control | P. aeruginosa |
| Tube 4 | C. albicans | S. epidermidis | Internal Control | C. glabrata |
| Tube 5 | Aspergillus spp. | C. tropicalis | Internal Control | Streptococcus spp. |
| Tube 6 | H. influenzae (type B) | S. pneumoniae | Internal Control | X |
| Tube 7 | X | L. monocytogenes | Internal Control | S. enterica |
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
The Bosphore Sepsis Panel Bundle Kit consists of a combination of Bosphore Sepsis Screening Kit (Box 1), and Bosphore ABR Screening Kit (Box 2).
The Bosphore Sepsis Screening Kit is a Real-Time PCR kit designed to detect and characterize Streptococcus agalactiae, Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis, Klebsiella pneumoniae, Staphylococcus spp., Serratia marcescens, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Candida krusei, Pseudomonas aeruginosa, Candida albicans, Staphylococcus epidermidis, Enterobacter cloacae, Candida glabrata, Aspergillus spp., Candida tropicalis, Escherichia coli, Streptococcus spp., Haemophilus influenzae (type B), Listeria monocytogenes, Candida parapsilosis, Salmonella enterica, Klebsiella oxytoca, Proteus mirabilis, Enterobacter (klebsiella) aerogenes, Neisseria meningitidis, Fusarium spp., Campylobacter spp., Candida auris, Streptococcus pyogenes in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
The Bosphore ABR Screening Kit is a Real-Time PCR kit designed to detect and characterize resistance genes for New Delhi Metallo-beta-lactamase (NDM), Oxacillinase-48-like (OXA 48), K. pneumoniae carbapenemase (KPC), Imipenemase (IMP), Verona Integron-Enconded Metallo-beta-lactamase (VIM), Cefotaximase (CTX-M), Mobilized colistin resistance-1 (MCR-1), Vancomycin resistance A (Van A), Vancomycin resistance B (Van B), Methicillin resistance gene (mecA) in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 | |
| Box 1 | PCR Master Mix 1 | S. agalactiae
(cfB gene) |
S. aureus
(nucA gene) |
S. pneumoniae
(lytA gene) |
E. faecalis
(DDL gene) |
| PCR Master Mix 2 | K. pneumoniae
(Hemolysin gene) |
Staphylococcus spp.
(tuf gene) |
S. marcescens
(16S rRNA/ITS/23S rRNA) |
E. faecium
(DDL gene) |
|
| PCR Master Mix 3 | A. baumannii (T6SS gene) | S. maltophilia
(ssrA gene) |
C. krusei
(ITS2 region) |
P. aeruginosa
(ecfX gene) |
|
| PCR Master Mix 4 | C. albicans
(ITS2 region) |
S. epidermidis
(sodA gene) |
E. cloacae
(rpoB gene) |
C. glabrata
(ITS2 region) |
|
| PCR Master Mix 5 | Aspergillus spp.
(18S rRNA gene) |
C. tropicalis
(ITS2 region) |
E. coli
(uidA gene) |
Streptococcus spp.
(16S rRNA/ ITS/23S rRNA gene) |
|
| PCR Master Mix 6 | H. influenzae (type B)
(bcs3 gene) |
L. monocytogenes
(hlyA gene) |
C. parapsilosis
(ITS2 region) |
S. enterica
(ttrA-ttrC gene) |
|
| PCR Master Mix 7 | K. oxytoca
(pehX gene) |
P. mirabilis
(ureR gene) |
E. (klebsiella) aerogenes
(gyrB gene) |
N. meningitidis
(ctrA gene) |
|
| PCR Master Mix 8 | Fusarium spp.
(TEF-1 gene) |
Campylobacter spp.
(16S rRNA gene) |
C. auris
(ITS1/5.8S rRNA/ITS2/ LSU rRNA gene) |
Internal Control | |
| PCR Master Mix 9 | – | – | S. pyogenes
(speB gene) |
Internal Control | |
| Component | FAM | HEX | Texas RED | Cy5 | |
| Box 2 | PCR Master Mix 1 | NDM | OXA 48 | – | KPC |
| PCR Master Mix 2 | IMP | VIM | CTX-M | MCR-1 | |
| PCR Master Mix 3 | Van A | Van B | mecA | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only Box 1 – PCR Master Mix 8, 9, and Box 2 – PCR Master Mix 3. The internal control can be added either during DNA extraction or the PCR step.
The Bosphore ABR Screening Kit is a Real-Time PCR kit designed to detect and characterize resistance genes for New Delhi metallo-beta-lactamase (NDM), oxacillinase-48-like (OXA 48), K. pneumoniae carbapenemase (KPC), imipenemase (IMP), verona integron-enconded metallo-beta-lactamase (VIM), cefotaximase (CTX-M), mobilized colistin resistance-1 (MCR-1), vancomycin resistance A (Van A), vancomycin resistance B (Van B), methicillin resistance gene (mecA) in human whole blood samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| FAM | HEX | Texas RED | Cy5 | |
| PCR Master Mix 1 | NDM | OXA 48 | – | KPC |
| PCR Master Mix 2 | IMP | VIM | CTX-M | MCR-1 |
| PCR Master Mix 3 | Van A | Van B | mecA | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only PCR Master Mix 3. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Sepsis Panel Bundle Kit is a Real-Time PCR kit designed to detect and characterize 32 different pathogens.
The target pathogens of the Bosphore Sepsis Screening Kit includes;
- Gram(-) A. baumannii, Campylobacter spp, E. aerogenes, E. cloacae, E. coli, H. influenzae type b, K. oxytoca, K. pneumoniae, N. meningitidis, P. aeruginosa, P. mirabilis, S. enterica, S. maltophilia, S. marcescens,
- Gram(+) E. faecalis, E. faecium, L. monocytogenes, Staphylococcus spp., S. aureus, S. epidermidis, Streptococcus spp., S. agalactiae, S. pneumoniae, S. pyogenes, and also
- Fungi Aspergillus spp., C. albicans, C. glabrata, C. krusei, C. auris, C. tropicalis, C. parapsilosis, Fusarium spp.
Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Internal and positive controls were integrated into the kit.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 | S. agalactiae | S. aureus | S. pneumoniae | E. faecalis |
| PCR Master Mix 2 | K. pneumoniae | Staphylococcus spp. | S. marcescens | E. faecium |
| PCR Master Mix 3 | A. baumannii | S. maltophilia | C. krusei | P. aeruginosa |
| PCR Master Mix 4 | C. albicans | S. epidermidis | E. cloacae | C. glabrata |
| PCR Master Mix 5 | Aspergillus spp. | C. tropicalis | E. coli | Streptococcus spp. |
| PCR Master Mix 6 | H. influenzae (type B) | L. monocytogenes | C. parapsilosis | S. enterica |
| PCR Master Mix 7 | K. oxytoca | P. mirabilis | E. (Klebsiella) aerogenes | N. meningitidis |
| PCR Master Mix 8 | Fusarium spp. | Campylobacter spp. | C. auris | Internal Control |
| PCR Master Mix 9 | X | X | S. pyogenes | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter for only PCR Master Mix 8 and 9. The internal control can be added either during DNA extraction or the PCR step.
