Automated NA Extraction

IVD CE

The initial procedure for all downstream molecular biology applications is nucleic acid extraction. After a targeted organism’s DNA or RNA has been extracted, a variety of standard molecular genetic assays and studies are carried out. DNA/RNA isolation often entails the breakdown of the cell membrane and cell wall, the deactivation of nuclease activity, the removal of proteins, the concentration of nucleic acids, and the proper preservation of the extracted DNA/RNA. 

Extraction is composed of 4 main steps named as; lysis (release of DNA by degrading the cells and the structures bound to membrane, separation, and denaturation of the proteins from DNA/RNA), binding (binding of DNA/RNA on a surface), washing (removal of unwanted contaminants such as salt, protein, etc.) and elution (recovery of DNA/RNA from the surface-bound). 

Anatolia offers several automated nucleic acid extraction systems operating on the magnetic-bead extraction method. Magnetic extraction technology is based on the separation of magnetic particle-bound DNA/RNA from cell components with the help of a magnet. The separation procedure, in which the magnetic particles are used as solid support, is an effective method adaptable to automated systems. It is faster and simpler compared to other methods. This method enables the ease of DNA/RNA extraction on a large scale. After the sample is treated with lysis solutions and proteinase enzyme and the DNA/RNA move out of the cell, free DNA/RNA is bound to the suitable carriers-magnetic particles and forms a magnetic complex. The removal of the undesired, bound contaminants is performed by washing the magnetic complex. In the last step, the DNA/RNA is eluted from the magnetic complex and stored for other downstream applications. 

Features

Automated extraction

Flexible solutions for diagnostics

Prefilled cartridges for 1-24 samples

Workflow

Technical Specifications

Thermal Protocol Single thermal protocol for all parameters
Sample Typing Body fluids including blood, serum, urine, sputum, swab, plasma, bronchoalveolar lavage (BAL) fluid, cerebrospinal fluid (CSF), cell culture
Shelf Life 18 months
Shipping / Storage -80°C / -20°C

Automated NA Extraction Kits

Magnesia 2448 Viral DNA/RNA Extraction Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Magnesia 2448 Viral DNA/RNA Extraction Kit has been designed for DNA/RNA extraction from body fluids and biological samples such as blood, serum, plasma, sputum, buffy coat, CSF, urine, swab on Magnesia 2448 Nucleic Acid Extraction and PCR Setup Robot.

Kit consists of Magnesia 2448 Nucleic Acid Extraction and PCR Setup Robot. The kit contains necessary reagents for extraction and plastic consumables and magnetic beads required for magnetic based insulation. Magnesia 2448 Viral DNA/RNA Extraction Kit is compatible with Magnesia 2448 Nucleic Acid Extraction and PCR Setup Robot and Bosphore Real-Time PCR kits.

The kit extracts and purifies both DNA and RNA from the samples at the same time and prepares it for use in any genomic application in a solution suitable for long-term storage. Starting volume for the sample is 500 µl and the DNA/RNA elution volume is 80 µl.

Magnesia 2448 Whole Blood Genomic DNA Extraction Kit is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.

Magnesia 2448 Whole Blood Genomic DNA Extraction Kit has been designed for automated extraction of whole blood DNA from biological sample materials.

Unio Bacterial DNA Extraction Kit has been designed for automated extraction of bacterial DNA from body fluids including blood, serum, urine, sputum, swab, plasma, broncho alveolar lavage (BAL) fluid, cerebrospinal fluid (CSF) and as well as cell culture supernatants by using Unio Extraction Systems (Anatolia Geneworks). The kit contains the required solutions for extraction as well as the plastic consumables for magnetic bead-based extraction. Starting sample volume is 100-200-400-600 µl and DNA recovery (elution) volume is 60-100-150-200 µL.

Operation Parameters
Operation Capacity 1-24 samples
Operation Duration 65 minutes
Sample Volume 100-200-400-600 µL
Elution Volume 60-100-150-200 µL

Unio Nucleic Acid Extraction Versatile Kit has been designed for automated extraction of DNA/RNA from body fluids including various biological sample materials (serum, blood, plasma, nasopharyngeal swabs, oropharyngeal swabs, tissues) by using Unio Extraction Systems (Anatolia Geneworks). The kit contains the required solutions for extraction as well as the plastic consumables for magnetic bead-based extraction. Depending on the type of protocol, starting sample volume ranges between 100 to 600 µl and DNA/RNA recovery (elution) volume ranges between 60 to 200 µL. Required time ranges between 16 to 75 minutes depending on the required protocol.

Operation Parameters
Operation Capacity 1-24 Samples
Operation Duration 16-75 minutes
Sample Volume 100-200-400-600 µL
Elution Volume 60-100-150-200 µL

Unio Viral DNA/RNA Extraction Kit 600 µl has been designed for automated extraction of viral DNA/RNA from all body fluids from various biological sample materials (serum, plasma, nasopharyngeal swabs, oropharyngeal swabs) by using Unio Extraction Systems (Anatolia Geneworks). The kit contains the required solutions for extraction as well as the plastic consumables for magnetic bead based extraction.  Starting sample volume is 600 µl and DNA/RNA recovery (elution) volume is 60-100-150-200 µL.

Operation Parameters
Operation Capacity 1-24 Samples
Operation Duration 65 minutes
Sample Volume 600 µL
Elution Volume 60-100-150-200 µL

Unio Whole Blood Genomic DNA Extraction Kit has been designed for automated extraction of genomic DNA from blood samples by using Unio Extraction Systems (Anatolia Geneworks). The kit contains the required solutions for extraction as well as the plastic consumables for magnetic bead based extraction. Starting sample volume is 100-200-400 µl and DNA recovery (elution) volume is 60-100-150-200 µL. Yield of total genomic DNA from 400 µl fresh whole blood is 5-15 µg in 60-200 µl of elution buffer.

Operation Parameters
Operation Capacity 1-24 samples
Operation Duration 65 minutes
Sample Volume 100-200-400 µL
Elution Volume 60-100-150-200 µL
Yield 5-15 µg
A260/280 Ratio 1.7-1.9

Unio 96 Nucleic Acid Extraction Versatile Kit has been designed and developed for automated extraction of DNA/RNA from different sources including body fluids originating from different biological sample materials (serum, blood, plasma, nasopharyngeal swabs, oropharyngeal swabs) by using Unio 96 Extraction & PCR Setup System (Anatolia Geneworks). The kit contains the solutions and magnetic beads required for the extraction. Starting sample volume is 200/400 µl and DNA/RNA recovery (elution) volume ranges from 60-200 µL depending on the protocol type.

Operation Parameters
Operation Capacity 1-96 Samples
Operation Duration 65 minutes-120 minutes
Sample Volume 200/400 µL
Elution Volume 60-200 µL

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