Real-Time PCR Kits
Tropical Pathogens
Tropical Pathogens consist of bacteria, viruses, or parasite species infectious to humans and animals, causing Tropical Diseases prevalent in or unique to tropical or subtropical regions. These pathogens are usually spread by insects such as mosquitos and flies.
Hot climates in these regions and larger volumes of rain are supporting the formation of breeding grounds, causing a larger number and variety of natural reservoirs and animal diseases with the possibility to transmit to humans. Approximately 1.5 million die each year because of tropical infectious and parasitic diseases.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Lesion swabs, whole blood, serum, plasma, throat swabs, urine, CSF and saliva |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Tropical Pathogens Kits
Bosphore Monkeypox Detection Kit v2 has been designed to detect the Monkeypox virus in human biological samples including lesion swabs, whole blood, serum, throat swabs, and saliva. Recommended biological specimens for laboratory diagnosis of Monkeypox are skin lesion material, including swabs of lesion surface and/or exudate, roofs from multiple lesions, or lesion crusts. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters. A region within the F3L gene is amplified and fluorescence detection is accomplished using the FAM filter.
| FAM | HEX | Cy5 |
| Monkeypox virus | Exogenous Internal Control | Endogenous Internal Control |
An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the exogenous internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
An endogenous internal control based on the detection with the CY5 of human endogenous nucleic acid sequence (F5 gene) present in the human genome has been employed to check extraction, PCR inhibition and sampling or application errors.
Bosphore CCHFV Quantification Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the S segment of CCHFV from serum and plasma samples. Fluorescence detection is performed using FAM, and HEX filters. CCHFV RNA is amplified, and fluorescence detection is performed using the FAM filter.
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or the PCR step.
Bosphore Dengue Virus Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 3’ UTR gene of Dengue virus serotypes 1, 2, 3 and 4 from serum, plasma, saliva, and urine samples. Fluorescence detection is performed using FAM and HEX filters. Dengue virus RNA is amplified and fluorescence detection is performed using the FAM filter.
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or the PCR step.
Bosphore Dengue Virus Genotyping Kit v1 detects and discriminates dengue virus serotypes 1-2-3 and 4 RNA in human biological samples including serum, plasma, saliva and urine. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters in two PCR tubes.
| FAM | HEX | Cy5 | |
| Tube 1
|
Dengue virus serotype 1 | Dengue virus serotype 4 | Internal Control |
| Tube 2
|
Dengue virus serotype 3 | Dengue virus serotype 2 | Internal Control |
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes with PCR Master Mix 1 and 2. The internal control is added during PCR step.
Bosphore Chikungunya Virus Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Chikungunya Virus Detection Kit v1 detects all the genotypes of the chikungunya virus RNA in various types of human samples such as serum, plasma, cerebrospinal fluid, tissue, swab, feces, etc. A region within the envelope protein gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added either during PCR step or in the RNA extraction step.
Bosphore Malaria Detection Kit v1 detects, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Malaria Detection Kit v1 detects Plasmodium species DNA in human biological samples, encompassing all the P. malariae, P. vivax, P. falciparum and P. ovale genotypes. Fluorescence detection is accomplished using the FAM filter. Detection Mix contains the specific primers required to detect malaria DNA. The amplification data of the malaria is detected qualitatively with the SYBR Green filter.
Bosphore Malaria Detection Kit v2 detects and characterizes Plasmodium species DNA in human biological samples such as whole blood, serum, or plasma, encompassing P. malariae, P. vivax, P. falciparum and P. ovale genotypes. A region within 18S rRNA encoding gene is amplified and fluorescence detection is accomplished using FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with HEX filter. The Internal Control can be added either during DNA extraction or the PCR step.
Bosphore Malaria Genotyping Kit v1 detects and discriminates Plasmodium species in human body fluids such as serum, plasma, saliva, urine etc. Kit targets 18S rRNA encoding gene within the Plasmodium genome. Analytic sensitivity is 1084 IU/ml for Plasmodium falciparum, 2456 copies/ml for Plasmodium vivax, 4200 copies/ml for Plasmodium malariae, and 3261 IU/ml for Plasmodium ovale. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters in two PCR tubes.
In the first PCR tube with PCR Master Mix 1, the Plasmodium falciparum genome is amplified, and fluorescence detection is accomplished using the FAM filter The Plasmodium malariae genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the second PCR tube with PCR Master Mix 2, the Plasmodium vivax genome is amplified, and fluorescence detection is accomplished using the FAM filter and the Plasmodium ovale genome is amplified and fluorescence detection is accomplished using the HEX filter.
An Internal Control has been integrated into the kit to check PCR inhibition. The Internal Control amplification data is detected with the CY5 filter in both PCR tubes with PCR Master Mix 1 and 2. The Internal Control is added during the PCR step.
Bosphore Zika Virus Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Zika Virus Detection Kit v1 detects Zika virus RNA in human biological samples such as blood, serum or plasma, body fluids, urine, tissue, swap specimens. A region within the polyprotein gene of Zika virus genome is amplified and detected with FAM filter.
Internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Dengue – Chikungunya Detection Kit detects and characterizes Dengue virus and Chikungunya virus RNA in human biological samples such as serum, plasma, saliva and urine. Fluorescence detection is accomplished by using HEX, Texas Red and Cy5 filters. The analytical detection (LOD) was found to be 8 copies/reaction for the Dengue virus and 7 copies/reaction for the Chikungunya virus.
An internal control has been integrated into the kit in order to check extraction, PCR inhibition, and application mistakes. The internal control can be added either during extraction or PCR step.
| HEX | Cy5 | Texas Red |
| Dengue virus | Chikungunya virus | Internal Control |
Bosphore Dengue-Malaria Detection Kit v1 detects and characterizes Plasmodium falciparum, Plasmodium vivax DNA, and Dengue virus RNA in human biological samples including whole blood and plasma samples. Plasmodium falciparum and Plasmodium vivax are amplified, and fluorescence detection is accomplished using the FAM filter. Dengue virus is amplified, and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check DNA/RNA extraction, PCR inhibition, and application failures. The amplification data of the internal control is detected with Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
| FAM | HEX | CY5 |
| P. falciparum/
P. vivax |
Dengue virus | Internal Control |
Bosphore Bosphore Zika-Dengue-Chikungunya Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the polyprotein gene of Zika virus, Dengue virus and Chikungunya virus from whole blood, serum, plasma, urine, and swab samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Zika virus RNA is amplified, and fluorescence detection is performed using the FAM filter. Dengue virus RNA is amplified, and fluorescence detection is performed using the HEX filter. Chikungunya virus RNA is amplified, and fluorescence detection is performed using the Cy5 filter.
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filter. The internal control can be added either during RNA extraction or the PCR step.
| Component | FAM |
HEX |
Texas RED | Cy5 |
| PCR Master Mix | Zika virus (Polyprotein gene) | Dengue virus
(Polyprotein gene) |
Internal Control | Chikungunya virus
(Polyprotein gene) |
Bosphore Lyme Disease Detection Kit has been designed to detect Borrelia burgdorferi, Borrelia afzelii and Borrelia garinii DNA extracted from human biological samples including whole blood, CSF and serum. Fluorescence detection is accomplished by using the FAM and HEX filters. A region within the “RecA” gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
| FAM | HEX |
| Lyme disease
(Borrelia burgdorferi / Borrelia afzelii / |
Internal Control |
Bosphore Plasmodium falciparum Quantification Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Plasmodium falciparum Quantification Kit v1 detects and quantitates Plasmodium falciparum DNA in human serum or plasma, encompassing all the P. falciparum sub-genotypes. A region within the 18S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter.
Bosphore West Nile Virus Quantification Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 3’ UTR region of West Nile virus RNA from serum, plasma, urine, whole blood, and CSF samples. Fluorescence detection is performed using FAM, and HEX filters.
West Nile virus RNA is amplified and fluorescence detection is performed using the FAM filter. Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during RNA extraction or the PCR step.
Bosphore JEV Detection Kit v1 detects and characterizes the Japanese encephalitis virus in human biological samples. Fluorescence detection is accomplished using the FAM and HEX filters. The analytical detection (LOD) was found to be 26 copies/reaction.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition, and application errors. Positive controls were added to the kit for qualitative comparison.
Bosphore Naegleria fowleri Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 18S ribosomal RNA gene of Naegleria fowleri from human biological samples. Fluorescence detection is performed using FAM and HEX filters. Naegleria fowleri DNA is amplified and fluorescence detection is performed using the FAM filter.
| Component | FAM | HEX |
| PCR Master Mix | Naegleria fowleri | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or the PCR step.
Tropical Fever Panel Kits
Bosphore Tropical Fever Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Plasmodium falciparum, Plasmodium malariae, Salmonella enterica, Plasmodium vivax, Dengue virus, Chikungunya virus, West Nile virus, Leptospira spp., and Plasmodium ovale DNA and RNA in human biological samples including whole blood and plasma. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filter. The internal control can be added either during DNA/RNA extraction or the PCR step.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED (Gene) | Cy5
(Gene) |
| PCR Master Mix 1 | Plasmodium falciparum
(18S ribosomal RNA) |
Plasmodium malariae
(18S ribosomal RNA) |
Internal Control | Salmonella enterica
(TTR site) |
| PCR Master Mix 2 | Plasmodium vivax
(18S ribosomal RNA) |
Dengue virus
(3’UTR) |
Internal Control | Chikungunya virus
(Envelope protein gene) |
| PCR Master Mix 3 | West Nile virus
(3’UTR) |
Plasmodium ovale
(18S ribosomal RNA) |
Internal Control | Leptospira spp.
(lipl32 gene) |
Bosphore Tropical Fever Panel Kit v2 detects and characterizes Plasmodium falciparum, Plasmodium malariae, Salmonella enterica, Plasmodium vivax, Dengue virus, Chikungunya virus, West Nile virus and Plasmodium ovale DNA and RNA in human biological samples such as whole blood and plasma samples. Fluorescence detection is accomplished by using FAM, HEX, Texas RED, and Cy5 filters.
Plasmodium falciparum is amplified, and fluorescence detection is accomplished using the FAM filter, Plasmodium malariae is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica is amplified, and fluorescence detection is accomplished using the Cy5 filter in the first tube with PCR Master Mix 1.
In the Second tube, Plasmodium vivax is amplified, and fluorescence detection is accomplished using the FAM filter, Dengue virus is amplified, and fluorescence detection is accomplished using the HEX filter and Chikungunya virus is amplified and fluorescence detection is accomplished using the Cy5 filter with PCR Master Mix 2.
In the third tube, the West Nile virus is amplified, and fluorescence detection is accomplished using the FAM filter, Plasmodium ovale is amplified and fluorescence detection is accomplished using the HEX filter with PCR Master Mix 3.
An Internal Control has been integrated into the kit to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with the Texas Red filter. The Internal Control can be added either during DNA extraction or the PCR step.
