Real-Time PCR Kits
Tropical Pathogens Kits
Tropical diseases are affecting a large number of people in regions such as Sub-Saharan Africa, South-East Asia, Eastern Mediterranean, Western Pacic and the America.
The infections that cause death such as West Nile virus transmitted by mosquitoes and Crimean Congo hemorrhagic fever transmitted by ticks, which is brought to the agenda with the deaths it causes at various times every year are among tropical pathogens.
Features
Workflow

Technical Specifications
Thermal Protocol | Single thermal protocol for all parameters |
Sample Typing | Whole blood and blood culture |
Shelf Life | 18 Months |
Channels | FAM, HEX/VIC, Cy5 |
Shipping / Storage | -80°C / -20°C |
Tropical Pathogens Kits
Bosphore CCHFV Quantification Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore CCHFV Quantification Kit v1 detects and quantitates all the four genotypes of CCHF virus RNA in human serum and plasma samples. A region within the S segment of CCHFV genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5/HEX/JOE filter. The internal control is added in PCR step.
Bosphore Chikungunya Virus Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Chikungunya Virus Detection Kit v1 detects all the genotypes of the chikungunya virus RNA in various types of human samples such as serum, plasma, cerebrospinal fluid, tissue, swab, feces, etc. A region within the envelope protein gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added either during PCR step or in the RNA extraction step.
Bosphore Dengue Virus Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Dengue Virus Detection Kit v1 detects dengue virus serotypes 1-2-3 and 4 RNA in human serum or plasma. A region within the 3’ UTR gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the CY5/HEX/JOE filter. The internal control is added during PCR step.
Bosphore Dengue Virus Genotyping Kit v1 detects and discriminates dengue virus serotypes 1-2-3 and 4 RNA in human biological samples including serum, plasma, saliva and urine. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters in two PCR tubes.
FAM | HEX | Cy5 | |
Tube 1
|
Dengue virus serotype 1 | Dengue virus serotype 4 | Internal Control |
Tube 2
|
Dengue virus serotype 3 | Dengue virus serotype 2 | Internal Control |
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes with PCR Master Mix 1 and 2. The internal control is added during PCR step.
Bosphore Dengue – Chikungunya Detection Kit detects and characterizes dengue virus and chikungunya virus RNA in human biological samples such as serum, plasma, saliva and urine. Fluorescence detection is accomplished by using HEX, Texas Red and Cy5 filters. The analytical detection (LOD) was found to be 8 copies/reaction for the Dengue virus and 7 copies/reaction for the Chikungunya virus.
An internal control has been integrated into the kit in order to check extraction, PCR inhibition, and application mistakes. The internal control can be added either during extraction or PCR step. Positive controls were included in the kit for qualitative comparison.
HEX | Cy5 | Texas Red |
Dengue virus | Chikungunya virus | Internal Control |
Bosphore Lyme Disease Detection Kit has been designed to detect Borrelia burgdorferi, Borrelia afzelii and Borrelia garinii DNA extracted from human biological samples including whole blood, CSF and serum. Fluorescence detection is accomplished by using the FAM and HEX filters. A region within the “RecA” gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
FAM | HEX |
Lyme disease
(Borrelia burgdorferi / Borrelia afzelii / |
Internal Control |
Bosphore Malaria Detection Kit v1 detects, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Malaria Detection Kit v1 detects Plasmodium species DNA in human biological samples, encompassing all the P. malariae, P. vivax, P. falciparum and P. ovale genotypes. Fluorescence detection is accomplished using the FAM filter. Detection Mix contains the specific primers required to detect malaria DNA. The amplification data of the malaria is detected qualitatively with the SYBR Green filter.
Bosphore Malaria Genotyping Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Malaria Genotyping Detection Kit v1 detects and discriminates Plasmodium species in human body fluids such as serum, plasma, saliva, urine etc. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters in two PCR tubes.
In the first PCR tube PCR Master Mix 1, Plasmodium falciparum genome is amplified and fluorescence detection is accomplished using the FAM filter and Plasmodium malariae genome is amplified and fluorescence detection is accomplished using the HEX filter.
Bosphore Plasmodium falciparum Quantification Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Plasmodium falciparum Quantification Kit v1 detects and quantitates Plasmodium falciparum DNA in human serum or plasma, encompassing all the P. falciparum sub-genotypes. A region within the 18S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter.
Bosphore Monkeypox Detection Kit v1 has been designed to detect monkeypox virus in human biological samples including lesion swab, whole blood, serum. Recommended biological specimens for laboratory diagnosis of monkeypox are skin lesion material, including swabs of lesion surface and/or exudate, roofs from multiple lesions, or lesion crusts [1]. Fluorescence detection is accomplished using the FAM and HEX filters. A region within the F3L gene is amplified and fluorescence detection is accomplished using the FAM filter.
FAM | HEX |
Monkeypox virus | Internal Control |
An internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
REFERENCES
[1] https://www.who.int/publications/i/item/WHO-MPX-laboratory-2022.1
Bosphore West Nile Virus Quantification Kit v2, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore West Nile Virus Quantification Kit v2 detects and quantitates West Nile virus RNA in human serum or plasma A region within the 3’ UTR region is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter.
In the second PCR tube PCR Master Mix 2, Plasmodium vivax genome is amplified and fluorescence detection is accomplished using the FAM filter and Plasmodium ovale genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes with PCR Master Mix 1 and 2. The internal control is added during PCR step.
Bosphore JEV Detection Kit v1 detects and characterizes the Japanese encephalitis virus in human biological samples. Fluorescence detection is accomplished using the FAM and HEX filters. The analytical detection (LOD) was found to be 26 copies/reaction.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition, and application errors. Positive controls were added to the kit for qualitative comparison.
Bosphore Zika Virus Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Zika Virus Detection Kit v1 detects Zika virus RNA in human biological samples such as blood, serum or plasma, body fluids, urine, tissue, swap specimens. A region within the polyprotein gene of Zika virus genome is amplified and detected with FAM filter.
Internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during nucleic acid extraction step or PCR step.