SARS-CoV-2 & Variant

Bosphore SARS-CoV-2 / Flu / RSV Panel Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. 

Bosphore SARS-CoV-2/Flu/RSV Panel Kit detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A&B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. 

Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by two regions of the virus (Orf1ab and N gene nCoV specific) from a single channel FAM. RSV A&B is detected by HEX channel. Influenza A&B is detected by Cy5 channel. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. Internal control is detected by Texas Red channel. 

Bosphore SARS-CoV-2/Flu/RSV Panel Kit v2 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A, influenza B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and oral fluids. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.

SARS-CoV-2 is detected by three regions of the virus in a single reaction with Master Mix 1: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. RSV A&B, influenza A and influenza B are detected and discriminated with Master Mix 2.

Bosphore SARS-CoV-2 Variant Detection Kit v1 detects and characterizes A570D, P681H, and Y144del mutations of SARS-CoV-2, which are some of the common variants observed in the UK (Lineage B.1.1.7), in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM and HEX filters. Detection of A570D, P681H, and Y144del mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore SARS-CoV-2 Variant Detection Kit v2 detects and characterizes N501Y, P681H, and E484K mutations of SARS-CoV-2, which are some of the common mutations observed in the UK (Lineage B.1.1.7), South Africa (Lineage B.1.351) and Brazil (Lineage B.1.1.28), in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. Detection of N501Y, P681H, and E484K mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (GAPDH) present in human genome has been employed in order to check nucleic acid extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore SARS-CoV-2 Variant Detection Kit v3 detects and characterizes N501Y, P681H, and E484K mutations of SARS-CoV-2, which are some of the common mutations observed in the UK (Lineage B.1.1.7), South Africa (Lineage B.1.351) and Brazil (Lineage B.1.1.28), in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. Detection of N501Y, P681H, and E484K mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (GAPDH) present in human genome has been employed in order to check nucleic acid extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore SARS-CoV-2 Variant Detection Kit v4 detects and characterizes L452R and E484Q mutations of SARS-CoV-2, which are two of the defining mutations in Lineage B.1.617 in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM, HEX, and Cy5 filters. Detection of L452R and E484Q mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check nucleic acid extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore SARS-CoV-2 Variant Detection Kit v5 detects and characterizes D796Y and N856K mutations of SARS COV-2, which are two of the defining mutations in Omicron (Lineage B.1.1.529), in human respiratory samples including nasopharyngeal, oropharyngeal swabs. Fluorescence detection is accomplished using HEX and Cy5 filters.

Detection of D796Y and N856K mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check nucleic acid extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore SARS-CoV-2 Screening & Variant Kit v3 detects and characterizes N501Y and E484K mutations of SARS-CoV-2 which are some of the common mutations observed in the UK (Lineage B.1.1.7), South Africa (Lineage B.1.351) and Brazil (Lineage B.1.1.28), N gene region and Orf1ab which is used to discriminate SARS-CoV-2 specifically in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. Detection of N501Y, E484K mutations and N gene/Orf1ab is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check nucleic acid extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit detects and characterizes 2019-nCoV in human respiratory samples.

Fluorescence detection is accomplished using FAM and HEX filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the Orf1ab target region is used to discriminate 2019-nCoV specifically. In the first PCR tube with PCR Master Mix 1, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter. In the second PCR tube with PCR Master Mix 2, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the FAM filter.

An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter in each tube. The internal control can be added either during RNA extraction or PCR step.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v2 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters. 2019-nCoV is detected by two regions of the virus in two separate reactions: E gene is used for screening purpose, where 2019-nCoV and also the closely related coronaviruses are detected, and the Orf1ab target region is used to discriminate 2019-nCoV specifically. In PCR Master Mix, 2019-nCoV target region is amplified and fluorescence detection is accomplished using FAM filter, 2019-nCoV screening region (E gene) is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with HEX filter.

The internal control can be added either during RNA extraction or PCR step.

In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v3 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.

Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, and the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.

An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The internal control can be added either during RNA extraction or PCR step.

In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v4 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.

Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.

An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.

Bosphore Novel Coronavirus (2019-nCoV) Detection Kit v7 detects and characterizes 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.

Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.

An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.

Bosphore N501Y Detection Kit v1 detects and characterizes A570D, P681H, and Y144del mutations of SARS-CoV-2, which are some of the common variants observed in the UK (Lineage B.1.1.7), in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM and HEX filters. Detection of A570D, P681H, and Y144del mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.

Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.