Real-Time PCR Kits
Respiratory Pathogens
Respiratory tract infections are typically divided into upper and lower respiratory tract infections which occur mainly in the nose, throat affecting upper respiratory system, windpipe, airways and lungs affecting lower respiratory system. From clinical point of view; symptoms such as nasal obstruction, nasal discharge, sore throat, cough, irritability occur as upper respiratory tract infections, symptoms such as bronchitis and pneumonia occur as lower tract infections.
When there is no adequate treatment, bronchitis, pneumonia, emphysema, asthma and bronchiectasis may occur. Respiratory infections include a diverse group of bacterial, viral and fungal infections which makes the respiratory diseases are very contagious which can spread through different routes of transmission, including contact, droplet, and airborne. Viral causative agents of respiratory infections include rhinovirus, adenovirus, coxsackievirus, parainfluenza virus, influenza virus, respiratory syncytial virus and human metapneumovirus. Bacterial causative agents of respiratory infections include Streptococcus, Chlamydia, Mycoplasma and Gonococcus. Fungal causative agents of respiratory infections include opportunistic fungal pathogens such as Candida and Aspergillus.
Features
Workflow

Technical Specifications
Thermal Protocol | Single thermal protocol for all parameters |
Sample Types | Respiratory samples such as nasopharyngeal swab specimens, sputum, nasopharyngeal aspirate, bronchoalveolar lavage and tissue samples |
Shelf Life | 18 Months |
Channels | FAM, HEX, Texas Red, Cy5, Quasar 705, ROX |
Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Respiratory Pathogens Kits
Bosphore Bordetella pertussis Detection Kit v1 detects Bordetella pertussis DNA including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. The analytic sensitivity of the kit is 1 copy/reaction. IS481 region in Bordetella pertussis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
The internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with the Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Component | Texas RED | Cy5 |
PCR Master Mix | Internal Control | Bordetella pertussis |
Bosphore B. pertussis/parapertussis Genotyping Kit detects and characterizes Bordetella pertussis and Bordetella parapertussis from biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, nasopharyngeal swab samples. Bordetella pertussis DNA is amplified and fluorescence detection is performed using the Cy5 filter, and Bordetella parapertussis DNA is amplified and fluorescence detection is performed using the FAM filter. An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition, and application errors. Kit also contains positive controls.
Component | FAM | HEX | Texas RED | Cy5 |
PCR Master Mix | Bordetella parapertussis | Internal Control | – | Bordetella pertussis |
Bosphore MERS-CoV Detection Kit v1 is a Real-Time PCR kit designed to detect and characterize Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in human respiratory samples including nasopharyngeal swabs, oropharyngeal swabs, and saliva samples. Orf1ab and UpE gene of MERS-CoV are detected in a single reaction. Fluorescence detection is performed using FAM, HEX, and Cy5 filters.
An Internal Control has been integrated into the kit to check RNA extraction, PCR inhibition, sampling, or application problems. The amplification data of the Internal Control is detected with the Cy5 filter.
Component | FAM (Gene) | HEX (Gene) | Cy5 (Gene) |
PCR Master Mix | MERS-CoV (Orf1ab gene) |
MERS-CoV (UpE gene) |
Internal Control (RNase P) |
Bosphore Bocavirus Detection Kit v1 detects bocavirus DNA in human biological samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, nose swab, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. The analytic sensitivity of the kit is 40 copies/reaction. A specific region of NP1 gene in bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Component | FAM | Cy5 |
PCR Master Mix | Bocavirus | Internal Control |
Bosphore H1N1 Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore H1N1 Detection Kit v1 detects pandemic H1N1 influenza A virus RNA (novel influenza A H1N1/2009 H1N1 virus) and seasonal H1N1 influenza A virus RNA in human serum or plasma, respiratory samples and nasopharyngeal specimens. Pandemic H1N1 influenza A virus RNA is amplified and detected with FAM filter and seasonal H1N1 influenza A virus RNA is amplified and detected with HEX filter.
Internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore H1N1 Detection Kit v3 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the influenza A, influenza B, pandemic H1N1 (novel influenza A H1N1/2009 H1N1 virus), and seasonal influenza A from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, and Cy5 filters.
FAM | HEX | Cy5 | |
PCR Master Mix 1 | Pandemic H1N1 | Seasonal Influenza A | Internal Control |
PCR Master Mix 2 | Influenza A | Influenza B | Internal Control |
The internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Human Metapneumovirus Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Nucleoprotein gene of Human Metapneumovirus from biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, and nasopharyngeal swab samples. Fluorescence detection is performed using HEX and Cy5 filters. Human Metapneumovirus RNA is amplified and fluorescence detection is performed using the HEX filter.
Component | HEX (Gene) | Cy5 |
PCR Master Mix | Human Metapneumovirus (Nucleoprotein) | Internal Control |
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or the PCR step.
Bosphore Human Parainfluenza Viruses (HPIVs) Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Human Parainfluenza Viruses (HPIVs) Detection Kit v1 detects and parainfluenza virus RNA (genotypes 1, 2, 3 and 4 in human biological samples such as serum or plasma, urine, respiratory samples, BOS, tissue, swab, nasopharyngeal specimens. A region from parainfluenza genome is amplified and fluorescence detection is achieved in 2 different wells. Parainfluenza 1 is detected with FAM filter and parainfluenza 3 is detected with Texas Red filter in the 1st well. Parainfluenza 2 is detected with HEX filter and parainfluenza 4 is detected with Cy5 filter in the 2nd well.
An internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter in the 1st well and with FAM filter in the 2nd well. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Parechovirus Detection Kit v1 detects parechovirus RNA in human biological samples. Parechovirus RNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the CY5 filter. The internal control is added during PCR step.
Bosphore Respiratory Syncytial Virus (RSV) Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Respiratory Syncytial Virus (RSV) Detection Kit v1 detects Respiratory Syncytial Virus (RSV) RNA in human biological samples such as serum, plasma, urine, respiratory samples, BOS, tissue, swab, encompassing all RSV genotypes. A region from RSV genome is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Streptococcus pyogenes (Group A) Detection Kit is a Real-Time PCR kit and IVD CE marked according to 98/79/EC Directive. It detects and characterizes the Streptococcus pyogenes (Group A) from oropharyngeal and nasopharyngeal swab samples. Streptococcus pyogenes (Group A) DNA is amplified, and fluorescence detection is performed using the Texas RED filter.
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter.
Bosphore Rhinovirus Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Rhinovirus Detection Kit v1 detects rhinovirus RNA including all subtypes in human respiratory samples such as nasopharyngeal swab specimens, sputum, nasopharyngeal aspirate, bronchoalveolar lavage and tissue samples. A region of rhinovirus genome is amplified and detected with HEX filter.
The internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Chlamydia pneumoniae Detection Kit v1 detects Chlamydia pneumoniae DNA in human biological samples, naspharyngeal swab, nasopharyngeal aspirate, tracheal aspirate, bronchoalveolar lavage, sputum and culture. Chlamydia pneumoniae DNA is amplified and fluorescence detection is accomplished using FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during PCR step.
Bosphore Haemophilus influenzae Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Haemophilus influenzae Detection Kit v1 detects Haemophilus influenzae DNA in human biological samples. A specific region in Haemophilus influenzae genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore K. pneumoniae Detection Kit v1 detects Klebsiella pneumoniae DNA in human biological samples. DNA of pathogen is amplified and fluorescence detection is accomplished using FAM filter.
An internal control has been integrated into the kit to check PCR inhibition. The internal control is added during the PCR step and its amplification data is detected with the HEX filter.
Bosphore L. pneumophila Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore L. pneumophila Detection Kit v1 detects Legionella pneumophila DNA in human biological samples, encompassing all the genotypes. A specific region in Legionella pneumophila genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Moraxella catarrhalis Detection Kit v1 detects Moraxella catarrhalis DNA in human biological samples. Moraxella catarrhalis DNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the CY5 filter. The internal control is added during PCR step.
Bosphore Mycoplasma pneumoniae Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore M. pneumoniae Detection Kit v1 detects M. pneumoniae DNA in human biological samples, encompassing all the genotypes. A region within the 16S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the FAM filter. The internal control is added in PCR step.
Bosphore Pneumocystis jirovecii Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Pneumocystis jirovecii Detection Kit v1 detects Pneumocystis jirovecii DNA in human biological samples, encompassing all the genotypes. A specific region partially encoding large subunit ribosomal RNA in Pneumocystis jirovecii genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Streptococcus pneumoniae Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore S.pneumoniae Detection Kit v1 detects Streptococcus pneumoniae DNA in human biological samples. Streptococcus pneumoniae DNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during PCR step.
Bosphore Staphylococcus aureus Detection Kit v1 detects and characterizes Staphylococcus aureus in human respiratory samples. Fluorescence detection is accomplished by using the HEX and Cy5 filters.
An internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Respiratory Pathogens Panel Kits
Bosphore RSV-Flu Panel Kit detects and characterizes influenza A, influenza B and RSV A/B in human respiratory samples in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters. Influenza A genome is amplified and fluorescence detection is accomplished using FAM filter, influenza B genome is amplified and fluorescence detection is accomplished using Cy5 filter, RSV A/B genome is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition, and application mistakes. Amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
FAM | HEX | Texas Red | Cy5 |
Influenza A | RSV A/B | IC | Influenza B |
Bosphore ADEP Panel Kit detects and characterizes enterovirus, parechovirus, and adenovirus in human biological samples including tissue/biopsy specimen, BAL, nasopharyngeal swab and aspirate, tracheal aspirate, sputum and CSF. The test is accomplished in a single tube qualitatively, encompassing all the subtypes of enterovirus, parechovirus, and adenovirus. A region in enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, a region in parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, and a region in adenovirus genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step. The amplification data of the internal control is detected with the Texas Red filter.
FAM | HEX | Texas Red | Cy5 |
Enterovirus | Parechovirus | IC | Adenovirus |
Bosphore Influenza A-B Detection Kit v1 detects Influenza A RNA and Influenza B RNA in human serum, plasma, respiratory samples, and nasopharyngeal specimens. The analytic sensitivity for Influenza A is 15 copies/reaction and for Influenza B is 2 copies/reaction. Influenza A virus RNA is amplified and detected with FAM filter and Influenza B virus RNA is amplified and detected with HEX filter.
The endogenous internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during the nucleic acid extraction step or PCR step.
Bosphore Influenza A-B-C Detection Panel Kit v1 detects and characterizes Influenza A, Influenza B, and Influenza C in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters. Influenza A genome is amplified, and fluorescence detection is accomplished using FAM filter. Influenza B genome is amplified, and fluorescence detection is accomplished using HEX filter. Influenza C is amplified, and fluorescence detection is accomplished using Cy5 filter. An internal control has been integrated into the kit to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Influenza Typing Kit v1 detects and discriminates against Influenza A virus, Influenza B virus, pandemic H1N1 influenza A virus (novel influenza A H1N1/2009 H1N1 virus), and Influenza A H3 virus in human respiratory samples including nasopharyngeal and oropharyngeal swabs. Fluorescence detection is accomplished using FAM, HEX and Cy5 filters.
An internal control for master mix 1, has been integrated into the kit to check RNA extraction, PCR inhibition and application errors. The internal control is added during the nucleic acid extraction step or PCR step.
Internal control for master mix 2, based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition and sampling or application errors. For both master mix 1 and 2, internal control is detected by the Cy5 channel.
FAM | HEX | CY5 | |
Master Mix 1 | Influenza A | Influenza B | Internal Control |
Master Mix 2 | Pandemic H1N1 | Influenza A H3 | Internal Control (RNase P) |
Bosphore Influenza Typing Kit v2 detects and discriminates pandemic H1N1 influenza A virus (novel influenza A H1N1/2009 H1N1 virus), Influenza A H3 virus, Influenza A H5 virus, Influenza A H7 virus, in human respiratory samples including nasopharyngeal and oropharyngeal swabs. Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters.
An internal control for Master Mix 1, based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition and sampling or application errors. For PCR Master Mix 1, internal control is detected by Cy5 channel.
An internal control for Master Mix 2, has been integrated into the kit to check RNA extraction, PCR inhibition and application errors. The internal control is added during the nucleic acid extraction step or PCR step. For PCR Master Mix 2, internal control is detected by HEX channel.
FAM | HEX | Texas RED | Cy5 | |
Master Mix 1 | Pandemic H1N1 virus | Influenza A H3 virus | X | Internal Control (RNase P) |
Master Mix 2 | X | Internal Control | Influenza A H5 virus | Influenza A H7
virus |
Bosphore Bacterial Pneumonia CAP Bundle Kit detects and discriminates Chlamydophila pneumoniae, Legionella pneumophila/longbeachae, Mycoplasma pneumoniae, H. influenzae, M. catarrhalis, S. pneumoniae, and S. aureus bacteria in human biological samples including throat swab, nasopharyngeal swab, nasopharyngeal aspirate, tracheal aspirate, bronchoalveolar lavage, sputum, biopsy (tissue) samples and culture in three PCR tubes. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.
FAM | HEX | Texas RED | Cy5 | |
Tube 1
Box-1 |
Chlamydophila pneumoniae | Internal Control | Legionella pneumophila/
longbeachae |
Mycoplasma pneumoniae |
Tube 1
Box-2 |
H. influenzae | M. catarrhalis | S. pneumoniae | Internal Control |
Tube 2
Box-2 |
– | S. aureus | – | Internal control |
An internal control has been integrated into the kit to check DNA extraction, PCR inhibition, and application mistakes. The internal control can be added either during the DNA extraction or PCR step.
Bosphore Bacterial Pneumonia CAP Bundle Kit is composed of Bosphore Atypical CAP Panel Kit and Bosphore Bacterial Pneumonia Mini Panel Kit.
Bosphore Respiratory Pathogens Panel Kit v5 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Internal Control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the Internal Control is detected with the Texas RED and Cy5 filters. The Internal Control can be added either during DNA/RNA extraction or the PCR step.
Component | FAM (Gene) |
HEX (Gene) |
Texas RED (Gene) |
Cy5 (Gene) |
PCR Master Mix 1 | Klebsiella pneumoniae (Hemolysin gene) |
Influenza B
(Segment 8/NS1) |
Internal Control | Mycoplasma pneumoniae (Adhesin P1) |
PCR Master Mix 2 | Parainfluenza 1 (HN gene) |
Parainfluenza 2 (N gene) |
Internal Control | Parainfluenza 4 (F glycoprotein) |
PCR Master Mix 3 | Enterovirus (5 ‘UTR) |
Human metapneumovirus A/B (Nucleoprotein) | Internal Control | – |
PCR Master Mix 4 | Influenza A (MP2 gene) |
RSV A/B (N gene) |
Parainfluenza 3 (HN gene) |
Internal Control |
PCR Master Mix 5 | Bocavirus (NP1 gene) |
Rhinovirus (5 ‘UTR) |
Coronavirus 229E (N gene) |
Internal Control |
PCR Master Mix 6 | Pandemic H1N1 influenza A (HA site) |
Seasonal H1N1 influenza A (HA site) |
Internal Control | Salmonella enterica (TTR Site) |
PCR Master Mix 7 | Adenovirus (Hexon gene) |
Moraxella catarrhalis (COP B) |
Internal Control | Bordetella pertussis (IS481) |
PCR Master Mix 8 | Haemophilus influenzae type B (BCS3 gene) |
Parechovirus (5 ‘UTR) |
Internal Control | – |
PCR Master Mix 9 | Influenza C (M Gene) |
Staphylococcus aureus (Nuc A) |
Pneumocystis jirovecii (mt-LSU RNA) |
Internal Control |
PCR Master Mix 10 | – | Streptococcus pneumoniae (Lyt A) |
Legionella pneumophila (MIP) |
Internal Control |
PCR Master Mix 11 | Coronavirus OC43 (N gene) |
Coronavirus
NL63 |
Internal Control | Coronavirus HKU1 (1ab polyprotein) |
Bosphore Respiratory Pathogens Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Internal Control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the Internal Control is detected by the filters specified in the table. The Internal Control can be added either during DNA/RNA extraction or the PCR step.
Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
PCR Master Mix 1 | Parainfluenza 1
(HN gene) |
Parainfluenza 2
(N gene) |
Internal Control | Parainfluenza 4
(F glycoprotein) |
PCR Master Mix 2 | Enterovirus
(5 ‘UTR) |
Human metapneumovirus A/B
(Nucleoprotein) |
Internal Control | – |
PCR Master Mix 3 | Influenza A
(MP2 gene) |
RSV A/B
(N gene) |
Parainfluenza 3
(HN gene) |
Internal Control |
PCR Master Mix 4 | Bocavirus
(NP1 gene) |
Rhinovirus
(5 ‘UTR) |
Coronavirus 229E
(N gene) |
Internal Control |
PCR Master Mix 5 | Coronavirus OC43
(N gene) |
Coronavirus NL63
(N gene) |
Internal Control | Coronavirus HKU1
(1ab polyprotein) |
PCR Master Mix 6 | H. influenza type B
(BCS3 gene) |
Influenza B
(Segment 8/NS1) |
Internal Control | M. pneumoniae
(P1 Adhesin) |
PCR Master Mix 7 | Pandemic H1N1
(HA site) |
Parechovirus
(5 ‘UTR) |
Internal Control | Adenovirus
(Hexon gene) |
Bosphore Respiratory Pathogens Panel Kit v7 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
FAM | HEX | Texas RED | Cy5 | |
PCR Master Mix 1 | Pandemic H1N1 | RSV A/B | Parainfluenza 3 | Internal Control |
PCR Master Mix 2 | Enterovirus | Human metapneumovirus | Internal Control | Adenovirus |
PCR Master Mix 3 | Bocavirus | Rhinovirus | Coronavirus 229E | Internal Control |
PCR Master Mix 4 | Parainfluenza 1 | Parainfluenza 2 | Internal Control | Parainfluenza 4 |
PCR Master Mix 5 | Influenza A | Parechovirus | Internal Control | Influenza B |
PCR Master Mix 6 | Coronavirus OC43 | Coronavirus NL63 | Internal Control | Coronavirus HKU1 |
PCR Master Mix 7 | SARS-CoV-2 | Internal Control | – | – |
An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 2, 4, 5, and 6 and; the Cy5 filter in PCR Master Mix 1 and 3. The internal control can be added either during DNA extraction or the PCR step.
An endogenous internal control based on the detection with the HEX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check extraction, PCR inhibition, and sampling or application errors. The endogenous internal control amplification data is detected with the HEX filter in PCR Master Mix 7.
Bosphore Respiratory Pathogens Panel Kit v8 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 1, 2, 5, 6, and 7; the Cy5 filter in PCR Master Mix 3 and 4; and the FAM filter in PCR Master Mix 8. The internal control can be added either during DNA/RNA extraction or the PCR step.
An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check extraction, PCR inhibition, and sampling or application errors. The endogenous internal control amplification data is detected with the Cy5 filter in PCR Master Mix 9.
Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
PCR Master Mix 1 | Parainfluenza 1
(HN gene) |
Parainfluenza 2
(N gene) |
Internal Control | Parainfluenza 4
(F glycoprotein) |
PCR Master Mix 2 | Enterovirus
(5 ‘UTR) |
Human metapneumovirus A/B
(Nucleoprotein) |
Internal Control | – |
PCR Master Mix 3 | Influenza A
(MP2 gene) |
RSV A/B
(N gene) |
Parainfluenza 3
(HN gene) |
Internal Control |
PCR Master Mix 4 | Bocavirus
(NP1 gene) |
Rhinovirus
(5 ‘UTR) |
Coronavirus 229E
(N gene) |
Internal Control |
PCR Master Mix 5 | Coronavirus OC43
(N gene) |
Coronavirus NL63
(N gene) |
Internal Control | Coronavirus HKU1
(1ab polyprotein) |
PCR Master Mix 6 | Pandemic H1N1
(HA site) |
Parechovirus
(5 ‘UTR) |
Internal Control | Adenovirus
(Hexon gene) |
PCR Master Mix 7 | Haemophilus influenzae type B (BCS3 gene) | Influenza B
(Segment 8/NS1) |
Internal Control | Mycoplasma pneumoniae (Adhesin P1) |
PCR Master Mix 8 | Internal Control | Streptococcus pneumoniae
(Lyt A) |
Legionella pneumophila
(MIP) |
Bordetella pertussis
(IS481) |
PCR Master Mix 9 | Influenza A-H3
(HA gene) |
Influenza A-H1
(MP2) |
– | Internal Control (RNase P) |
Bosphore Respiratory Pathogens Panel Kit v9 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Component/Strip Set | FAM | HEX | Texas Red | Cy5 |
PCR Master Mix 1 | Influenza A | RSV A/B | Endogenous
Internal Control (RNase P) |
Adenovirus |
PCR Master Mix 2 | SARS-CoV-2 | Rhinovirus | S. pyogenes | Influenza B |
An endogenous internal control based on the detection of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, DNA/RNA extraction, PCR inhibition, sampling, or application problems. The amplification data of the internal control is detected with the Texas RED filter of PCR Master Mix 1.
*Available in Strip format
Bosphore Respiratory Pathogens Panel Kit v10 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swabs and nasopharyngeal swabs. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Component/Strip Set | FAM | HEX | Texas Red | Cy5 |
PCR Master Mix 1 | Parainfluenza 1 | Parainfluenza 2 | Exogenous
Internal Control (Synthetic DNA) |
Parainfluenza 4 |
PCR Master Mix 2 | Enterovirus
|
Human metapneumovirus A/B
|
Streptococcus pneumoniae | – |
PCR Master Mix 3 | Influenza A
|
RSV A/B | – | Parainfluenza 3 |
PCR Master Mix 4 | Bocavirus | Rhinovirus | Coronavirus 229E | Bordetella pertussis |
PCR Master Mix 5 | Coronavirus OC43
|
Coronavirus NL63 | – | Coronavirus HKU1 |
PCR Master Mix 6 | Pandemic H1N1 | Parechovirus | Legionella pneumophila | Adenovirus |
PCR Master Mix 7 | Haemophilus influenzae type B | Influenza B | SARS-CoV-2 | Mycoplasma pneumoniae |
PCR Master Mix 8 | Influenza A-H3 | Influenza A-H1 | – | Endogenous
Internal Control |
An exogenous internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the exogenous internal control is detected with the Texas RED filter of PCR Master Mix 1. Internal control can be added during the DNA/RNA extraction step.
An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check RNA extraction, PCR inhibition, sampling, or application errors. The amplification data of the endogenous internal control is detected with the Cy5 filter in PCR Master Mix 8.
*Available in Strip format
Bosphore Respiratory Pathogens Panel Kit v2s is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Component/Strip Set | FAM | HEX | Texas Red | Cy5 |
PCR Master Mix 1 | Parainfluenza 1 | Parainfluenza 2 | Exogenous
Internal Control (Synthetic DNA) |
Parainfluenza 4 |
PCR Master Mix 2 | Enterovirus
|
Human metapneumovirus A/B
|
Streptococcus pneumoniae | – |
PCR Master Mix 3 | Influenza A
|
RSV A/B | – | Parainfluenza 3 |
PCR Master Mix 4 | Bocavirus | Rhinovirus | Coronavirus 229E | Bordetella pertussis |
PCR Master Mix 5 | Coronavirus OC43
|
Coronavirus NL63 | – | Coronavirus HKU1 |
PCR Master Mix 6 | Pandemic H1N1 | Parechovirus | Legionella pneumophila | Adenovirus |
PCR Master Mix 7 | Haemophilus influenzae type B | Influenza B | SARS-CoV-2 | Mycoplasma pneumoniae |
PCR Master Mix 8 | Influenza A-H3 | Influenza A-H1 | Streptococcus pyogenes | Endogenous
Internal Control |
An exogenous internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the exogenous internal control is detected with the Texas RED filter of PCR Master Mix 1. The internal control can be added during the DNA/RNA extraction step.
An endogenous internal control based on the detection with the Cy5 filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed in order to check DNA/RNA extraction, PCR inhibition, sampling, or application errors. The amplification data of the endogenous internal control is detected with the Cy5 filter in PCR Master Mix 8.
Bosphore Respiratory Pathogens Maxi Panel detects and characterizes adenovirus, bocavirus, Bordetella pertussis, coronavirus 229E, coronavirus OC43, coronavirus NL63, coronavirus HKU1, Chlamydia pneumoniae, enterovirus, Haemophilus influenzae type B, influenza A, influenza B, influenza C, Klebsiella pneumoniae, Legionella pneumophila, Legionella pneumophila / longbeachae, Mycoplasma pneumoniae, Moraxella catarrhalis, metapneumovirus, RSV A/B, pandemic H1N1 influenza A, parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, parechovirus, Pneumocystis jirovecii, rhinovirus, seasonal H1N1 influenza A, Salmonella enterica, Staphylococcus aureus, and Streptococcus pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
FAM | HEX | Cy5 | Texas Red | |
Tube 1 – Box 1 | K. pneumoniae | Influenza B | M. pneumoniae | Internal Control |
Tube 2 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
Tube 3 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
Tube 4 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
Tube 5 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
Tube 6 – Box 1 | Pandemic H1N1 | Seasonal H1N1 | S. enterica | Internal Control |
Tube 7 – Box 1 | Adenovirus | M. catarrhalis | B. pertussis | Internal Control |
Tube 8 – Box 1 | H. influenza type B | Parechovirus | – | Internal Control |
Tube 9 -Box 1 | Influenza C | S. aureus | Internal Control | P. jirovecii |
Tube 10 -Box 1 | – | S. pneumoniae | Internal Control | L. pneumophila |
Tube 11 -Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
Tube 1 – Box 2 | C. pneumoniae | Internal Control | M. pneumoniae | L. pneumophila / longbeachae |
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
Bosphore Respiratory Pathogens Mini Panel Kit detects and characterizes parainfluenza 1-2-3-4, enterovirus, metapneumovirus, influenza A-B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus 229E-HKU1-NL63-OC43, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae and Mycoplasma pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
FAM | HEX | Cy5 | Texas Red | |
Tube 1 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
Tube 2 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
Tube 3 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
Tube 4 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
Tube 5 – Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
Tube 6 – Box 1 | Pandemic H1N1 | Influenza B | Adenovirus | Internal Control |
Tube 7 – Box 1 | H. influenza type B | Parechovirus | Internal Control | |
Tube 8 – Box 1 | – | S. aureus | Internal Control | S. pneumoniae |
Bosphore Respiratory Pathogens Mini Panel Bundle Kit detects and characterizes parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, enterovirus, metapneumovirus, influenza A, influenza B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus OC43, coronavirus NL63, coronavirus HKU1, coronavirus 229E, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila / longbeachae, Chlamydophila pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
FAM | HEX | Cy5 | Texas Red | |
Tube 1 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
Tube 2 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
Tube 3 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
Tube 4 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
Tube 5 – Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
Tube 6 – Box 1 | Pandemic H1N1 | Influenza B | Adenovirus | Internal Control |
Tube 7 – Box 1 | H. influenza type B | Parechovirus | Internal Control | |
Tube 8 – Box 1 | – | S. aureus | Internal Control | S. pneumoniae |
Tube 1 – Box 2 | C. pneumoniae | Internal Control | M. pneumoniae | L. pneumophila / longbeachae |
Bosphore Respiratory Viral Basic Panel Kit detects and characterizes adenovirus, enterovirus, Legionella pneumophila, metapneumovirus, Mycoplasma pneumoniae, rhinovirus, and parainfluenza viruses (1, 2, 3, 4) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
FAM | HEX | Cy5 | Texas Red | |
Tube 1 | Enterovirus | Metapneumovirus | Adenovirus | Internal Control |
Tube 2 | Human parainfluenza 1/2/3/4 | Rhinovirus | Mycoplasma pneumoniae | Legionella pneumophila |
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
Bosphore Coronavirus Panel Kit v1 detects and characterizes Coronavirus 229E, Coronavirus HKU1, Coronavirus OC43 and Coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter, Coronavirus HKU1 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the second PCR tube with PCR Master Mix 2, Coronavirus OC43 genome is amplified and fluorescence detection is accomplished using the FAM filter, Coronavirus NL63 genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the Internal Control is detected with the HEX filter in PCR Master Mix 1, Texas Red filter in PCR Master Mix 2. The Internal Control can be added either during DNA extraction or PCR step.
Bosphore SARS-CoV-2/Flu/RSV Panel Kit is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which was later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B in a single tube from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva. Fluorescence detection uses FAM, HEX, Texas RED, and Cy5 filters. SARS-Cov-2 RNA is amplified, and fluorescence detection is performed using the FAM filter, RSV A&B RNA is amplified, and fluorescence detection is performed using the HEX filter, and Influenza A&B RNA is amplified, and fluorescence detection is performed using the Cy5 filter.
Component | FAM | HEX | Texas RED | Cy5 |
PCR Master Mix | SARS-CoV-2 | RSV A&B | Internal Control | Influenza A&B |
An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check RNA extraction, PCR inhibition, sampling, or application errors. Internal control is detected by the Texas RED filter.
Bosphore SARS-CoV-2/Flu/RSV Panel Kit v2 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A, influenza B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and oral fluids. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
SARS-CoV-2 is detected by three regions of the virus in a single reaction with Master Mix 1: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the Orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. RSV A&B, influenza A and influenza B are detected and discriminated with Master Mix 2.
FAM | HEX | Texas Red | Cy5 | |
PCR Master Mix 1 | Orf1ab
(SARS-CoV-2) |
Internal Control (RNase P) | N gene
(SARS-CoV-2) |
E gene
(SARS-CoV-2) |
PCFR Master Mix 2 | Influenza A | RSV A&B | – | Influenza B |
Bosphore SARS-CoV-2/Flu/RSV Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A&B from human respiratory samples including nasopharyngeal, oropharyngeal swabs, sputum and saliva.
Fluorescence detection is accomplished using FAM, HEX, Texas RED, Cy5, and Quasar 705 filters.
Component | FAM | HEX | Texas RED | Cy5
|
Quasar 705 |
PCR Master Mix | SARS-CoV-2 | RSV A&B | Internal Control | Influenza B | Influenza A |
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed in order to check RNA extraction, PCR inhibition, and sampling or application errors. Internal control is detected by the Texas RED channel.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the SARS-CoV-2, RSV A/B, influenza A/B, Enterovirus, Human Metapneumovirus A/B, Adenovirus, Human Parainfluenza viruses 1/2/3/4, Rhinovirus, Mycoplasma pneumoniae, and Legionella pneumophila from human respiratory samples including nasopharyngeal, oropharyngeal swabs, and saliva. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
FAM | HEX | Texas RED | Cy5 | |
PCR Master Mix 1 | SARS-CoV-2 | RSV A/B | Internal Control | Influenza A/B |
PCR Master Mix 2 | Enterovirus | Human Metapneumovirus A/B | Internal Control | Adenovirus |
PCR Master Mix 3 | Human Parainfluenza Viruses 1/2/3/4 | Rhinovirus | Legionella pneumophila | Mycoplasma pneumoniae |
An internal control based on the detection of human endogenous nucleic acid sequence present in the human genome has been employed to check DNA/RNA extraction, PCR inhibition, or application problems. The kit also contains positive controls.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v3 Real-Time PCR kit for in vitro diagnostics that detects and characterizes the specified gene regions of the following pathogens from human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Component | FAM | HEX | Texas RED | Cy5 |
PCR Master Mix 1 | SARS-CoV-2 | RSV A/B | Influenza A | Influenza B |
PCR Master Mix 2 | Enterovirus | Human metapneumovirus A/B | Internal Control | Adenovirus |
PCR Master Mix 3 | Human parainfluenza virus type 1/2/3/4 | Rhinovirus | Legionella pneumophila | Mycoplasma pneumoniae |
An internal control based on detecting human endogenous nucleic acid sequence (RNase P) present in the human genome has been employed to check nucleic acid extraction, PCR inhibition, and sampling or application errors. Amplification data of the internal control is detected with the Texas RED filter for PCR Master Mix 2.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v4 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
Component | FAM | HEX
|
Texas RED
|
Cy5
|
PCR Master Mix
1 |
Pandemic H1N1
|
Parechovirus
|
– | – |
PCR Master Mix
2 |
Parainfluenza 1
|
Rhinovirus
|
Exogenous
Internal Control
|
Mycoplasma pneumoniae |
PCR Master Mix
3 |
Enterovirus
|
Coronavirus NL63
|
Coronavirus 229E
|
Coronavirus HKU1
|
PCR Master Mix
4 |
Parainfluenza 4 | Endogenous
Internal Control |
Parainfluenza 2
|
Parainfluenza 3
|
PCR Master Mix
5 |
Coronavirus OC43
|
Human metapneumovirus A/B | Bocavirus
|
Adenovirus |
PCR Master Mix
6 |
SARS-CoV-2
|
RSV A/B
|
Influenza A
|
Influenza B |
An exogenous internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition, and application mistakes. The amplification data of the exogenous internal control is detected with the Texas RED filter in PCR Master Mix 2. The internal control can be added either during DNA/RNA extraction or the PCR step.
An endogenous internal control based on the detection with the HEX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, and application errors. The endogenous internal control amplification data is detected with the HEX filter in PCR Master Mix 4.
Bosphore SARS-CoV-2/Respiratory Pathogens Panel Kit v5 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human respiratory samples including oropharyngeal swab and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, ROX, and Cy5 filters.
Component | FAM
|
HEX
|
ROX
|
Cy5
|
PCR Master Mix 1 | Adenovirus
|
RSV A/B
|
Influenza A
|
Influenza B
|
PCR Master Mix 2 | Enterovirus
|
Human metapneumovirus A/B
|
Bocavirus
|
SARS-CoV-2
|
PCR Master Mix 3 | Pandemic H1N1
|
Rhinovirus
|
Mycoplasma pneumoniae | Coronavirus OC43/HKU1
|
PCR Master Mix 4 | Human parainfluenza virus type 1/2/3/4 | Parechovirus
|
Endogenous Internal Control | Coronavirus NL63/229E |
An endogenous internal control based on the detection with the ROX filter of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, extraction, PCR inhibition, and application errors. The endogenous internal control amplification data is detected with the ROX filter in PCR Master Mix 4.