Real-Time PCR Kits
Polyomaviruses
BK and JC viruses (BKV and JCV) are double-stranded DNA viruses belonging to the Polyomaviridae family. While primary infections typically occur during childhood and remain asymptomatic in healthy individuals, serological studies indicate that over 70% of adults carry antibodies against these viruses. However, BKV and JCV can reactivate in immunocompromised patients and during pregnancy, leading to severe clinical complications. Early and accurate diagnosis of viral reactivation is paramount to preventing severe disease and ensuring long-term organ survival, particularly in transplant recipients.
Anatolia offers high-sensitivity Bosphore Real-Time PCR kits for the precise detection and quantification of BKV and JCV. These kits are designed to work across a wide range of biological samples, including serum, plasma, urine, and CSF, providing reliable results for effective clinical monitoring.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Human bronchoalveolar lavage, whole blood, urine, plasma, cerebrospinal fluid, and amniotic fluid |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Polyomavirus Kits
Bosphore BKV Quantification Kit v1 detects and quantitates all genotypes of BK virus DNA in human serum, plasma and urine samples. The analytic sensitivity is 75 copies/ml. A region within the large T-antigen encoding gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control is added either in extraction or PCR step.
| FAM | HEX |
| BKV | Internal Control |
Bosphore JCV Quantification Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore JCV Quantification Kit v1 detects and quantitates JC virus DNA in human serum or plasma sample. A region within the large T-antigene encoding gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added either in extraction or PCR step.
Bosphore JCV-BKV Quantification Kit is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the VP2 genes of John Cunningham virus (JCV) and BK virus (BKV) from human bronchoalveolar lavage, whole blood, urine, plasma, cerebrospinal fluid, and amniotic fluid. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. JCV DNA is amplified, and fluorescence detection is performed using the FAM filter, BKV DNA is amplified, and fluorescence detection is performed using the HEX filter.
| Component | FAM (Gene) |
HEX (Gene) |
Cy5 |
| PCR Master Mix | JCV (VP2 gene) |
BKV (VP2 gene) |
Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step. When UNIO 448 System is used, all processing – including internal control handling – is managed automatically by UNIO 448 System.
This version of the kit is fully compatible with UNIO 448 System, which performs automated magnetic bead–based nucleic acid extraction, PCR setup, amplification, and fluorescence detection in a closed and contamination-controlled environment.
UNIO 448 software automatically recognizes barcoded reagents and applies the validated assay protocol for Bosphore JCV-BKV Quantification Kit. Before starting the run, the QR code located on the PCR kit must be scanned by the barcode reader of UNIO 448 System.
Bosphore JCV-BKV Detection Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the VP2 genes of John Cunningham virus (JCV) and BK virus (BKV) from serum, plasma, urine, tissue/biopsy, BAL, amniotic fluid and CSF samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. JCV DNA is amplified and fluorescence detection is performed using the FAM filter and BKV DNA is amplified and fluorescence detection is performed using the HEX filter.
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step.
