Real-Time PCR Kits
Helicobacter pylori Kits
Helicobacter pylori is an important human pathogen that infects up to 50% of the human population.
Helicobacter pylori is caused chronic inflammation in their localization area and it is the leading cause of peptic ulcers (commonly known as stomach ulcer, duodenum ulcer), gastritis and gastric cancer. More than 50% of world population has Helicobacter pylori in their superior gastrointestinal zone. Approximately 80 % of Helicobacter pylori infections are asymptomatic. Prevalent complains are burning pain in the superior abdomen, satiation feeling after slight nutrition, lack of appetite, sicchasia, vomit, black and bloody stool, tiredness and low blood count (hemogram).
H. pylori treatment is mostly performed by using clarithromycin which is a potent antibiotic. However, clarithromycin resistance of H. pylori is one of the main problems in treatment of infections. Various mutations are involved in the resistance mechanism. The prevalence of clarithromycin resistance has been increasing worldwide.
|Single thermal protocol for all parameters
|Serum and plasma
|FAM, HEX/VIC, Cy5
|Shipping / Storage
|-90°C / -20°C
Helicobacter pylori Kits
Bosphore Helicobacter pylori Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Helicobacter pylori Detection Kit v1 detects Helicobacter pylori DNA in human biological samples. A region within the 23S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during PCR step.
Bosphore Helicobacter pylori Genotyping Kit v1 is an in vitro diagnostic Real-Time PCR kit that detects and characterizes clarithromycin resistance point mutations A2142G, A2142C, and A2143G in the 23S RNA of Helicobacter pylori from stool, gastric tissue biopsy, biopsy specimens embedded in paraffin specimens. Fluorescence detection is performed using FAM, HEX, and Cy5 filters.
|PCR Master Mix 1
|Clarithromycin Resistance Mutation (A2142G)
|PCR Master Mix 2
|Clarithromycin Resistance Mutation (A2143G)
|Double Clarithromycin Resistance Mutation (A2142G and A2143G)
|PCR Master Mix 3
|Double Clarithromycin Resistance Mutation (A2142C and A2143G)
|Clarithromycin Resistance Mutation (A2142C)
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or the PCR step.