Real-Time PCR Kits
Fungi
Fungi are a diverse group of microorganisms that play a vital role in ecosystems but can also cause a range of infections in humans, particularly in immunocompromised individuals.
The genus Candida is one of the most common groups of fungal organisms responsible for hospital-acquired infections. It comprises more than 20 species. Infections caused by Candida are collectively referred to as candidiasis, which can be categorized into oropharyngeal candidiasis, vaginal candidiasis, and invasive candidiasis. Invasive candidiasis occurs when Candida species enter the bloodstream, leading to severe systemic infections.
Aspergillus, a genus of airborne fungi, is a natural ecosystem component. This genus includes more than 100 species, although only a few are pathogenic. These pathogenic species can cause hypersensitivity reactions, infectious diseases, and toxic effects when their asexual spores are inhaled. Aspergillus infections, collectively termed aspergillosis, include allergic bronchopulmonary aspergillosis (ABPA), chronic pulmonary aspergillosis, invasive aspergillosis, and skin aspergillosis. In immunocompromised or immunosuppressed individuals, including cancer patients and those with lung disease, Aspergillus infections can be life-threatening.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Blood, blood culture, epithelial cells, semen, urine, prostate secretions |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Fungi Kits
Bosphore Aspergillus Detection Kit v1 detects Aspergillus DNA in human biological samples, encompassing the most important genotypes of Aspergillus (Aspergillus fumigatus, Aspergillus ustus, Aspergillus flavus, Aspergillus versicolor, Aspergillus glaucus, Aspergillus niger, Aspergillus terreus, Aspergillus sclerotiorum, Aspergillus ochraceus, Aspergillus candidus, Aspergillus alliaceus, Aspergillus wentii, Aspergillus carbonarius, Aspergillus sydowii, Aspergillus oryzae, Aspergillus ficuum, Aspergillus silvaticus, Aspergillus nidulans, Aspergillus tamarii, Aspergillus proliferans, Aspergillus cervinus, Aspergillus restrictus, Aspergillus pseudoglaucus, Aspergillus sparsus, Aspergillus flavipes). The analytic sensitivity is 10 copies/µl. A region within the 18S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore C. albicans Detection Kit v1 detects C. albicans DNA in human biological samples such as blood, blood culture, epithelial cells, semen, urine, prostatic secretions. The analytic sensitivity is 100 copies/ml. A region within the Internal Transcribed Spacer 2 (ITS2) gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Candida Basic Panel Kit v2 detects and characterizes Candida albicans, Candida glabrata, Candida krusei and Candida parapsilosis in human biological samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, Candida albicans genome is amplified and fluorescence detection is accomplished using the FAM filter. Candida glabrata genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the second PCR tube with PCR Master Mix 2, Candida krusei genome is amplified and fluorescence detection is accomplished using the HEX filter. Candida parapsilosis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas Red filter in PCR Master Mix 1 and PCR Master Mix 2.
Bosphore C. auris Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the ITS1/5.8S rRNA and ITS2/LSU rRNA genes of Candida auris from whole blood, serum, plasma, nasal swab, armpit, and groin swab samples. Fluorescence detection is performed using Texas RED and Cy5 filters. C. auris DNA is amplified, and fluorescence detection is performed using the Texas RED filter.
| Component | Texas RED
(Gene) |
Cy5 |
| PCR Master Mix | Candida auris
(ITS1/5.8S rRNA and ITS2/LSU rRNA genes) |
Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Fungal Respiratory Detection Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Aspergillus spp., Mucor spp., and Pneumocystis pneumonia (PCP) (caused by Pneumocystis jirovecii) from human respiratory samples including BAL, sputum, tracheal aspirate, tissue/biopsy, throat swab, and nasopharyngeal swab. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
In PCR Master Mix, Aspergillus spp. DNA is amplified, and fluorescence detection is performed using the FAM filter; Mucor spp. DNA is amplified, and fluorescence detection is performed using HEX filter, and Pneumocystis jirovecii DNA is amplified, and fluorescence detection is performed using Texas RED filter.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
| PCR Master Mix | Aspergillus spp.
(18S rRNA) |
Mucor spp.
(rDNA operon) |
Pneumocystis jirovecii (PCP)
(mt-LSU RNA) |
Endogenous Internal Control
(RNase P) |
An endogenous internal control based on the detection of human endogenous nucleic acid sequence (RNase P gene) present in the human genome has been employed to check sampling, DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control are detected with the Cy5 filter of PCR Master Mix.
Bosphore Candida Screening & Typing Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Candida spp., Candida albicans, Candida tropicalis, Candida glabrata, and Candida parapsilosis from human whole blood, serum, and plasma samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
| PCR Master Mix 1 | – | Internal Control | Candida spp. (ITS region) | – |
| PCR Master Mix 2 | Candida albicans
(ITS region) |
Candida tropicalis
(ITS region) |
Candida glabrata
(ITS region) |
Candida parapsilosis
(ITS region) |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the HEX filter of PCR Master Mix 1. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Candida Screening & Typing Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Candida spp., Candida albicans, Candida tropicalis, Candida glabrata, Candida parapsilosis, Candida dubliniensis, Candida auris, and Candida krusei from human whole blood, serum, and plasma samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM (Gene) | HEX (Gene) | Texas RED (Gene) | Cy5 (Gene) |
| PCR Master Mix 1 | – | Internal Control | Candida spp. (ITS region) | – |
| PCR Master Mix 2 | Candida albicans
(ITS region) |
Candida tropicalis
(ITS region) |
Candida glabrata
(ITS region) |
Candida parapsilosis (ITS region) |
| PCR Master Mix 3 | Candida dubliniensis (ITS region) | Candida auris
(ITS region) |
Candida krusei (ITS region) | – |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the HEX filter of PCR Master Mix 1. The internal control can be added either during DNA extraction or the PCR step.
