Real-Time PCR Kits
Veterinary Products
The health of the stock must be maintained at the highest level in industrial livestock rearing not only for the welfare of the animals but also to reduce losses and expenses. All animals are susceptible to infectious illnesses, which limits output and necessitates protracted recovery times. A great strategy to stop the spread of infectious diseases is through routine scanning programs. As preventative measures, Real-Time PCR diagnosis technologies are quick, easy, economical, and produce highly accurate results for the disease of interest.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Animal body fluid samples (serum, plasma, whole blood) |
| Shelf Life | 18 Months |
| Channels | FAM, HEX |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Veterinary Products
Bosphore BTV Detection Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the NS gene of Bluetongue virus from blood, serum, and tissue samples. Fluorescence detection is performed using HEX and Cy5 filters. Bluetongue virus RNA is amplified, and fluorescence detection is performed using the HEX filter.
| Component | HEX (Gene) | Cy5 (Gene) |
| PCR Master Mix | Bluetongue virus NS Gene | Internal Control |
Internal control has been integrated into the kit to check PCR inhibition or application problems. The amplification data of internal control is detected with the Cy5 filter. The internal control can be added either during RNA extraction or the PCR step.
Bosphore BVDV Detection Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the 5’UTR region of Bovine viral diarrhea virus from blood, serum, and tissue samples. Fluorescence detection is performed using FAM and Cy5 filters. Bovine viral diarrhea virus RNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) | Cy5 (Gene) |
| PCR Master Mix | Bovine viral diarrhea virus 5’UTR region | Internal Control |
Internal Control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter. Internal Control can be added either during RNA extraction or the PCR step.
Bosphore LSDV Detection Kit v1 detects lumpy skin disease virus (LSDV) in bovine body fluids including serum, plasma, and whole blood. A region within the LSDV116 gene is amplified and fluorescence detection is accomplished using the FAM filter. The amplification data of the internal control is detected with the HEX filter.
Bosphore ASFV Detection Kit v2 is a Real-Time PCR kit for research use that detects and characterises the major capsid protein p72 gene of African Swine Fever Virus (ASFV) in swine samples including tissue, swab, serum, and whole blood with high analytical sensitivity, repeatability, and reproducibility. ASFV DNA is amplified, and fluorescence detection is performed using the FAM filter. Internal control, swine endogenous nucleic acid sequence (GAPDH) detected by the HEX channel, has been employed to check DNA extraction, PCR inhibition, and sampling or application errors.
Bosphore ASFV Detection Kit v2 successfully got the Validation Report from the Centro de Investigación en Sanidad Animal (CISA-INIA) as the European Union Reference Laboratory for African Swine Fever (EURL-ASF).
Bosphore ASFV Detection Kit v2.1 is a Real-Time PCR kit for research use that detects and characterizes the major capsid protein p72 gene of African Swine Fever Virus (ASFV) in samples including tissue (including tonsils, spleen, lymph nodes, kidneys, liver, lungs, bone marrow), oropharyngeal swabs, serum, and whole blood (with EDTA) from pigs, domestic swine and wild boars.
The kit is suitable for use with both individual and pooled samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. ASFV DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) | HEX (Gene) | Cy5 (Gene) |
| PCR Master Mix | ASFV (major capsid protein p72 gene) | Endogenous Internal Control (GAPDH) | Exogenous Internal Control (Synthetic DNA) |
An endogenous internal control based on the detection of the GAPDH gene in the genomes of pigs and wild boars has been employed to check DNA extraction, PCR inhibition, sampling, or application errors. The amplification data of the endogenous internal control is detected with the HEX filter.
An exogenous internal control has been integrated into the kit to detect DNA extraction issues, PCR inhibition, or application problems. The amplification data of the exogenous internal control is detected with the Cy5 filter of the PCR Master Mix. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Avian Influenza Virus Typing Kit v1 is a Real-Time PCR kit for research use that detects and characterizes M1 gene of Avian Influenza virus (AIV) and HA gene of AIV strains H3, H5, H7, and H9 from poultry cloacal swab and tracheal swab samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM (Gene) | HEX (Gene) | Texas RED (Gene) | Cy5 (Gene) |
| PCR Master Mix 1 | AIV H9 (HA gene) | AIV H3 (HA gene) | AIV H5 (HA gene) | Internal Control |
| PCR Master Mix 2 | AIV (M1 gene) | Internal Control | – | AIV H7 (HA gene) |
An exogenous internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 Filter for PCR Master Mix 1 and HEX filter for PCR Master Mix 2. The internal control can be added either during RNA extraction or the PCR step.
Bosphore PRRSV Detection Kit v1 is a research use Real-Time PCR kit that detects and characterizes the Open Reading frame (ORF) 6 and 7 of European Porcine Reproductive and Respiratory Syndrome Virus (EU PRRSV), 3’UTR of North American Porcine Reproductive and Respiratory Syndrome Virus (NA PRRSV) and NSP2 gene of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus (HP PRRSV) in porcine whole blood, serum, tissue and swab samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM (Gene) | HEX (Gene) | Texas RED (Gene) | Cy5 |
| PCR Master Mix 1 | EU PRRSV (ORF 6-7) | NA PRRSV
(3’UTR) |
HP PRRSV*
(NSP2) |
Internal Control |
*In the Bosphore PRRSV Detection Kit v1, HP PRRSV within the NA PRRSV lineage is targeted.
An exogenous internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Cy5 Filter. The internal control can be added either during RNA extraction or the PCR step.
