Real-Time PCR Kits
Plant Species Detection
Today, one of the biggest problems in the food industry is adulteration and the deterioration of the originality of food products. It is very difficult to detect the additives used in processed products without using advanced analysis methods.
Today, species identification is performed using anatomical separation of hair tissues, physical separation of adipose tissue, DNA hybridization, ELISA and PCR methods. However, many difficulties were encountered in the identification of species by immunological, morphological and electrophoretic methods, the species could not be determined or cases were encountered incorrectly. The fact that it can detect mixtures in processed and unprocessed products with an accuracy of up to 0.1% has made the Real-Time PCR method the most reliable method in this field. The PCR method, which is more sensitive, specific and fast, is accepted as the detection method of today and the future.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Processed and unprocessed food, feed, seed samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Plant Species Detection Kits
Bosphore CaMV Detection Kit v1 is a Real-Time PCR confirmation kit designed to detect the Cauliflower Mosaic Virus (CaMV) gene region in processed and unprocessed food and seed samples.
| Component | FAM (Gene) | HEX |
| PCR Master Mix | Cauliflower Mosaic Virus (CaMV) | Internal Control |
The 35S Promoter of Cauliflower Mosaic Virus (CaMV) is detected using the FAM filter. The kit also includes an internal control to monitor PCR inhibition.
Bosphore Plant Detection Kit v2 detects plant actin (ACT) gene with Cy5 filter in different samples (soy, corn/maize, cotton, canola, potato etc.) obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. A region within actin gene is amplified and fluorescence detection is accomplished using the Cy5 filter. The absolute LOD value of the kit is 5 copies.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Plant Detection Kit v3 detects plant actin (ACT) gene with Cy5 filter in different samples (soy, corn/maize, cotton, canola, potato etc.) obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. A region within actin gene is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Soy Species Detection Kit v1 detects soy specific lectin gene in different samples obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. A region within soy specific lectin gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Corn/Maize Species Detection Kit v1 detects corn/maize species alcohol dehydrogenase 1 (adh1) gene in different samples obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. A region within soy specific lectin gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Cotton Species Detection Kit v1 detects cotton species Sinapis Arabidopsis Homolog 7 (SAH7) gene in different samples obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. A region within soy specific Sinapis Arabidopsis Homolog 7 (SAH7) gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added in PCR step.
Bosphore Maize – Soy – Cotton Species Detection Kit v1 detects maize specific alcoholdeydrogenase 1 gene (adh1), cotton specific Sinapis Arabidopsis Homolog 7 (SAH7) gene, and soy specific lectin gene in different samples obtained from various matrices (product groups) such as all type of processed and non-processed food, feed, seed samples. The ADH1, SAH7, and lectin genes are amplified and fluorescence detection is accomplished using the FAM, HEX and Texas Red filters.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during PCR step.
Bosphore Maize-Soy-Cotton Species Detection Kit v1 detects genes specific to corn, cotton, and soybean through FAM, HEX, and Texas RED filters in various product groups including processed and unprocessed food, feed, and seed samples, respectively.
Bosphore Sugar Beet Species Detection Kit v1 detects sugar beet plant-specific gene by the FAM filter and internal control amplification data through the HEX filter in different samples such as processed and unprocessed food, feed, and seed samples.
Bosphore Oilseed Rape Species Detection Kit v1 detects oilseed rape plant-specific gene by the HEX filter in different samples such as processed and unprocessed food, feed, and seed samples. The amplification of the internal control is displayed with the CY5 filter.
Bosphore Rice Species Detection Kit v1 detects rice-specific phospholipase D (PLD) in different samples obtained from various matrices (product groups) such as all types of processed and non-processed food, feed, and seed samples. The PLD gene is amplified, and fluorescence detection is accomplished using the FAM filter.
| Component | FAM | HEX |
| PCR Master Mix 1 | Rice Phospholipase D (PLD) gene | Internal Control |
An internal control has been integrated into the kit to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during the PCR step.
