Real-Time PCR Kits
Food Safety Testing
Foodborne pathogens have always been a major concern for public health. Surveillance and identification of the source of the outbreak can be a challenge. The pathogens originating from farm animal or wildlife animal excrements first contaminate the groundwater. The contaminated source in turn contaminates every process and environment it is used. It is of utmost importance that the source must be identified as quickly as possible to control and stop the outbreak.
Bosphore Food Safety Testing Kits serve as sensitive, accurate and reliable tools for samples collected to help protect public health.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Processed and unprocessed food, feed, seed samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Food Safety Testing Kits
Bosphore Hepatitis A Detection Kit v2 detects hepatitis A RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Hepatitis A genome is amplified and fluorescence detection is accomplished using FAM filter. The analytical detection limit for Bosphore Hepatitis A Detection Kit v2 was found to be 21 copies/reaction.
A process control has been integrated into the kit to control extraction efficiency. The amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.
Bosphore Norovirus Detection Kit v2 detects norovirus GI and GII RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters. Norovirus genogroup GI genome is amplified and fluorescence detection is accomplished using FAM filter and norovirus genogroup GII genome is amplified and fluorescence detection is accomplished using HEX filter. Analytical detection limit for Bosphore Norovirus Detection Kit v2 was found to be 13 copies/reaction for norovirus GI and 20 copies/reaction for norovirus GII.
A process control has been integrated into the kit to control extraction efficiency. Amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.
Bosphore S. aureus Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates Staphylococcus aureus mecA gene from food samples. Fluorescence detection is performed using HEX and Cy5 filters. Staphylococcus aureus DNA is amplified, and fluorescence detection is performed using HEX filter.
| Component | HEX (Gene) | Cy5 |
| PCR Master Mix | Staphylococcus aureus (mecA gene) | Internal Control |
The kit includes Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore E. coli Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes uidA gene of Escherichia coli from food samples. Fluorescence detection is performed using FAM and HEX filters. Escherichia coli DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) | HEX |
| PCR Master Mix | Escherichia coli (uidA gene) | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. Internal control can be added to the PCR step.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore E. coli O157 Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the Z3276 gene of the E. coli O157 pathogen from food samples. Fluorescence detection is performed using HEX and Cy5 filters. E. coli O157 DNA is amplified, and fluorescence detection is performed using the HEX filter.
| Component | HEX (Gene) | Cy5 |
| PCR Master Mix | Escherichia coli O157 (Z3276 gene) | Internal Control |
The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Campylobacter Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the 16S rRNA gene of Campylobacter spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Campylobacter spp. DNA is amplified, and fluorescence detection is performed using HEX filter. The sensitivity/detection limit of Bosphore Campylobacter Food Testing Kit v1 is 8 DNA copies/reaction.
| Component | HEX (Gene) | Cy5 |
| PCR Master Mix | Campylobacter spp.
(16S rRNA) |
Internal Control |
The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control (IC) is performed using a Cy5 filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Salmonella Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the ttrA-ttrC gene of Salmonella spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Salmonella spp. DNA is amplified, and fluorescence detection is performed using Cy5 filter.
| Component | HEX | Cy5 (Gene) |
| PCR Master Mix | Internal Control | Salmonella spp. (ttrA-ttrC) |
The kit includes Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Salmonella typhimurium Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the stm4200 gene of Salmonella typhimurium from food and feed samples. Fluorescence detection is performed using FAM and HEX filters. Salmonella typhimurium DNA is amplified, and fluorescence detection is performed using the FAM filter. The detection limit for Bosphore S. typhimurium Food Testing Kit v1 was found to be 18.75 copies/reaction for Salmonella typhimurium.
| Component | FAM (Gene) | HEX |
| PCR Master Mix | Salmonella typhimurium (stm4200) | Internal Control |
The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Salmonella enteridits Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes sdfl gene of Salmonella enteridits from food and feed samples. Fluorescence detection is performed using HEX and Texas RED filters. Salmonella enteridits DNA is amplified, and fluorescence detection is performed using the HEX filter. Detection limit for Bosphore Salmonella enteridits Food Testing Kit v1 is 50 copies/ reaction.
| Component | HEX
(Gene) |
Texas RED |
| PCR Master Mix | Salmonella enteridits (sdfl) | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Texas RED filter. The internal control can be added either during DNA extraction or the PCR step.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore L. monocytogenes Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the hly gene of the L. monocytogenes pathogen from food samples. Fluorescence detection is performed using HEX and Cy5 filters. L. monocytogenes DNA is amplified, and fluorescence detection is performed using Cy5 filter. The sensitivity/detection limit of the Bosphore L. monocytogenes Food Testing Kit v1 kit is 12.5 DNA copies/reaction.
| Component | HEX | Cy5 (Gene) |
| PCR Master Mix | Internal Control | Listeria monocytogenes (hly) |
The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Shigella Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the ipaH gene of Shigella spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Shigella spp. DNA is amplified, and fluorescence detection is performed using the HEX filter.
| Component | HEX (Gene) | Cy5 |
| PCR Master Mix | Shigella spp. (ipaH gene) | Internal Control |
The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Staphylococcal Enterotoxins Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the SE genes that cause the production of Staphylococcal enterotoxin from food samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas Red | Cy5 |
| PCR Master Mix 1 | Staphylococcal Enterotoxin A (SEA) | Staphylococcal Enterotoxin B (SEB) | Staphylococcal Enterotoxin C (SEC) | Internal Control |
| PCR Master Mix 2 | Staphylococcal Enterotoxin D (SED) | Staphylococcal Enterotoxin E (SEE) | – | Internal Control |
Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter.
The method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, including milk and dairy products, and ready-to-eat desserts. For product groups that may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.
Bosphore C. botulinum Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the BoNT gene of Clostridium botulinum from food samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. Clostridium botulinum DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM | HEX | Cy5 |
| PCR Master Mix 1 | Botulinum neurotoxin A (BoNT/A) | Botulinum neurotoxin B (BoNT/B) | Internal Control |
| PCR Master Mix 2 | Botulinum neurotoxin E (BoNT/E) | Botulinum neurotoxin F (BoNT/F) | Internal Control |
Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter.
The pre-enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. The EX-Tract DNA solution and neutralization buffer included in the kit allow for rapid sample preparation from various food matrices. For product groups such as cocoa products, spices, tea and herbal teas, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.
Bosphore B. cereus Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the groEL gene of B. cereus from food samples. Fluorescence detection is performed using FAM and HEX filters. B. cereus DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) |
HEX |
| PCR Master Mix | Bacillus cereus (groEL gene) |
Internal Control |
Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the HEX filter. Internal Control can be added either during DNA extraction or the PCR step.
The enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, such as processed meat products, dough-based filled products, bread varieties, spices, ready-to-eat meals, ready-to-eat desserts, ready-to-eat salads, ready-to-eat appetizers, and infant formula. For product groups such as cocoa products, spices, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.
Bosphore C. perfringens Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the 16s rRNA gene of C. perfringens from food samples. Fluorescence detection is performed using FAM and HEX filters. C. perfringens DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) |
HEX |
| PCR Master Mix | Clostridium perfringens (16s rRNA) |
Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the HEX filter. Internal Control can be added either during DNA extraction or the PCR step.
The enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, such as processed meat products, dough-based filled products, bread varieties, spices, ready-to-eat meals, ready-to-eat desserts, ready-to-eat salads, ready-to-eat appetizers, and infant formula. For product groups such as cocoa products, spices, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.
Bosphore Vibrio Food Testing Kit v1 is a Real-Time PCR kit for research use only, which detects and characterizes OmpW gene from Vibrio cholerae, tlh gene from Vibrio parahaemolyticus, and vvhA gene from Vibrio vulnificus in food samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Vibrio cholerae DNA is amplified and fluorescence detection is performed using FAM filter, Vibrio parahaemolyticus DNA is amplified and fluorescence detection is performed using HEX filter, and Vibrio vulnificus DNA is amplified and fluorescence detection is performed using Cy5 filter.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix | Vibrio cholerae (OmpW gene) | Vibrio parahaemolyticus (tlh gene) | Internal Control | Vibrio vulnificus (vvhA gene) |
Internal control has been integrated into the kit to check PCR inhibition or application problems. The amplification data of the internal control is detected with the Texas RED filter.
The kit contains EX-Tract DNA solution and neutralization buffer.
Bosphore Coagulase Positive Staphylococci Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the CoA gene of Coagulase Positive Staphylococci from food samples. Fluorescence detection is performed using FAM and HEX filters. Coagulase Positive Staphylococci DNA is amplified, and fluorescence detection is performed using the FAM filter.
| Component | FAM (Gene) | HEX |
| PCR Master Mix | Coagulase Positive Staphylococci (CoA gene) | Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control is added during the PCR step.
The enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit, it allows for rapid sample preparation from various food matrices. For product groups such as cocoa products, spices, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the Unio B24 and Unio M32 extraction systems.
