Food Safety Testing

Bosphore Norovirus Detection Kit v2 detects norovirus GI and GII RNA from fruit and vegetable samples, sea food, meat, poultry and dairy products. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters. Norovirus genogroup GI genome is amplified and fluorescence detection is accomplished using FAM filter and norovirus genogroup GII genome is amplified and fluorescence detection is accomplished using HEX filter. Analytical detection limit for Bosphore Norovirus Detection Kit v2 was found to be 13 copies/reaction for norovirus GI and 20 copies/reaction for norovirus GII.

A process control has been integrated into the kit to control extraction efficiency. Amplification data of the process control is detected with the Cy5 filter. Also, positive control (external control-EC) has been integrated to the kit to control RT-PCR inhibition and to act as an RT-PCR positive control.

Bosphore S. aureus Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates Staphylococcus aureus mecA gene from food samples. Fluorescence detection is performed using HEX and Cy5 filters. Staphylococcus aureus DNA is amplified, and fluorescence detection is performed using HEX filter.

The kit includes Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore Salmonella Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the ttrA-ttrC gene of Salmonella spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Salmonella spp. DNA is amplified, and fluorescence detection is performed using Cy5 filter.

The kit includes Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore E. coli O157 Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the Z3276 gene of the E. coli O157 pathogen from food samples. Fluorescence detection is performed using HEX and Cy5 filters. E. coli O157 DNA is amplified, and fluorescence detection is performed using the HEX filter.

The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore Campylobacter Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the 16S rRNA gene of Campylobacter spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Campylobacter spp. DNA is amplified, and fluorescence detection is performed using HEX filter. The sensitivity/detection limit of Bosphore Campylobacter Food Testing Kit v1 is 8 DNA copies/reaction.

The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control (IC) is performed using a Cy5 filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore Salmonella typhimurium Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the stm4200 gene of Salmonella typhimurium from food and feed samples. Fluorescence detection is performed using FAM and HEX filters. Salmonella typhimurium DNA is amplified, and fluorescence detection is performed using the FAM filter. The detection limit for Bosphore S. typhimurium Food Testing Kit v1 was found to be 18.75 copies/reaction for Salmonella typhimurium.

The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore L. monocytogenes Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the hly gene of the L. monocytogenes pathogen from food samples. Fluorescence detection is performed using HEX and Cy5 filters. L. monocytogenes DNA is amplified, and fluorescence detection is performed using Cy5 filter. The sensitivity/detection limit of the Bosphore L. monocytogenes Food Testing Kit v1 kit is 12.5 DNA copies/reaction.

The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a HEX filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore Shigella Food Testing Kit v1 is a research-use Real-Time PCR kit that detects and differentiates the ipaH gene of Shigella spp. from food and feed samples. Fluorescence detection is performed using HEX and Cy5 filters. Shigella spp. DNA is amplified, and fluorescence detection is performed using the HEX filter.

The kit contains Internal Control (IC) to check DNA extraction, PCR inhibition, and application errors. Internal Control is added to the PCR reaction mixture. Fluorescence detection for Internal Control is performed using a Cy5 filter.

The kit contains EX-Tract DNA solution and neutralization buffer.

Bosphore Staphylococcal Enterotoxins Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the SE genes that cause the production of Staphylococcal enterotoxin from food samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter.

The method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, including milk and dairy products, and ready-to-eat desserts. For product groups that may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.

Bosphore C. botulinum Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the BoNT gene of Clostridium botulinum from food samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. Clostridium botulinum DNA is amplified, and fluorescence detection is performed using the FAM filter.

Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter.

The pre-enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. The EX-Tract DNA solution and neutralization buffer included in the kit allow for rapid sample preparation from various food matrices. For product groups such as cocoa products, spices, tea and herbal teas, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.

Bosphore B. cereus Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the groEL gene of B. cereus from food samples. Fluorescence detection is performed using FAM and HEX filters. B. cereus DNA is amplified, and fluorescence detection is performed using the FAM filter.

Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the HEX filter. Internal Control can be added either during DNA extraction or the PCR step.

The enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, such as processed meat products, dough-based filled products, bread varieties, spices, ready-to-eat meals, ready-to-eat desserts, ready-to-eat salads, ready-to-eat appetizers, and infant formula. For product groups such as cocoa products, spices, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.

Bosphore C. perfringens Food Testing Kit v1 is a Real-Time PCR kit for research use only that detects and characterizes the 16s rRNA gene of C. perfringens from food samples. Fluorescence detection is performed using FAM and HEX filters. C. perfringens DNA is amplified, and fluorescence detection is performed using the FAM filter.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the HEX filter. Internal Control can be added either during DNA extraction or the PCR step.

The enrichment stage of the method operates in compatibility with equipment that should be available in the microbiology section of any food analysis laboratory. Using the EX-Tract DNA solution and neutralization buffer included in the kit allows for rapid sample preparation from various food matrices, such as processed meat products, dough-based filled products, bread varieties, spices, ready-to-eat meals, ready-to-eat desserts, ready-to-eat salads, ready-to-eat appetizers, and infant formula. For product groups such as cocoa products, spices, food colorings, and colored flavorings, which may cause PCR inhibition during the DNA extraction stage, it is recommended to use the UNIO B24 and UNIO M32 extraction systems.