Real-Time PCR Kits
Respiratory Pathogens Kits
Respiratory tract infections are typically divided into upper and lower respiratory tract infections which occur mainly in the nose, throat affecting upper respiratory system, windpipe, airways and lungs affecting lower respiratory system. From clinical point of view; symptoms such as nasal obstruction, nasal discharge, sore throat, cough, irritability occur as upper respiratory tract infections, symptoms such as bronchitis and pneumonia occur as lower tract infections.
When there is no adequate treatment, bronchitis, pneumonia, emphysema, asthma and bronchiectasis may occur. Respiratory infections include a diverse group of bacterial, viral and fungal infections which makes the respiratory diseases are very contagious which can spread through different routes of transmission, including contact, droplet, and airborne. Viral causative agents of respiratory infections include rhinovirus, adenovirus, coxsackievirus, parainfluenza virus, influenza virus, respiratory syncytial virus and human metapneumovirus. Bacterial causative agents of respiratory infections include Streptococcus, Chlamydia, Mycoplasma and Gonococcus. Fungal causative agents of respiratory infections include opportunistic fungal pathogens such as Candida and Aspergillus.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Typing | Respiratory samples such as nasopharyngeal swab specimens, sputum, nasopharyngeal aspirate, bronchoalveolar lavage and tissue samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX/VIC, Texas Red/ROX, Cy5 |
| Shipping / Storage | -80°C / -20°C |
Respiratory Pathogens Kits
Bosphore RSV-Flu Panel Kit detects and characterizes influenza A, influenza B and RSV A/B in human respiratory samples in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters. Influenza A genome is amplified and fluorescence detection is accomplished using FAM filter, influenza B genome is amplified and fluorescence detection is accomplished using Cy5 filter, RSV A/B genome is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition, and application mistakes. Amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
| FAM | HEX | Texas Red | Cy5 |
| Influenza A | RSV A/B | IC | Influenza B |
Bosphore ADEP Panel Kit detects and characterizes enterovirus, parechovirus, and adenovirus in human biological samples including tissue/biopsy specimen, BAL, nasopharyngeal swab and aspirate, tracheal aspirate, sputum and CSF. The test is accomplished in a single tube qualitatively, encompassing all the subtypes of enterovirus, parechovirus, and adenovirus. A region in enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, a region in parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, and a region in adenovirus genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step. The amplification data of the internal control is detected with the Texas Red filter.
| FAM | HEX | Texas Red | Cy5 |
| Enterovirus | Parechovirus | IC | Adenovirus |
Bosphore Respiratory Viral Basic Panel Kit detects and characterizes adenovirus, enterovirus, Legionella pneumophila, metapneumovirus, Mycoplasma pneumoniae, rhinovirus, and parainfluenza viruses (1, 2, 3, 4) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | Enterovirus | Metapneumovirus | Adenovirus | Internal Control |
| Tube 2 | Human parainfluenza 1/2/3/4 | Rhinovirus | Mycoplasma pneumoniae | Legionella pneumophila |
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
Bosphore Bocavirus Detection Kit v1 detects bocavirus DNA in human biological samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, nose swab, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. The analytic sensitivity of the kit is 40 copies/reaction. A specific region of NP1 gene in bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Coronavirus Panel Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Coronavirus Panel Kit v1 detects and characterizes coronavirus 229E, coronavirus HKU1, coronavirus OC43 and coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter, coronavirus HKU1 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the second PCR tube with PCR Master Mix 2, coronavirus OC43 genome is amplified and fluorescence detection is accomplished using the FAM filter, coronavirus NL63 genome is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter in PCR Master Mix 1, Texas Red filter in PCR Master Mix 2. The internal control can be added either during DNA extraction or PCR step.
Bosphore H1N1 Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore H1N1 Detection Kit v1 detects pandemic H1N1 influenza A virus RNA (novel influenza A H1N1/2009 H1N1 virus) and seasonal H1N1 influenza A virus RNA in human serum or plasma, respiratory samples and nasopharyngeal specimens. Pandemic H1N1 influenza A virus RNA is amplified and detected with FAM filter and seasonal H1N1 influenza A virus RNA is amplified and detected with HEX filter.
Internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore H1N1 Detection Kit v3 detects pandemic H1N1 influenza A virus RNA (novel influenza A H1N1/2009 H1N1 virus), seasonal H1N1 influenza A virus RNA, Influenza A RNA and Influenza B RNA in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished in 2 PCR tubes.
| FAM | HEX | Cy5 | |
| Tube 1 | Pandemic H1N1 | Seasonal Influenza A | Internal Control |
| Tube 2 | Influenza A | Influenza B | Internal Control |
The internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Influenza A-B-C Detection Panel Kit v1 detects and characterizes influenza A, influenza B and influenza C in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters. Influenza A genome is amplified and fluorescence detection is accomplished using FAM filter, influenza B genome is amplified and fluorescence detection is accomplished using HEX filter, influenza C is amplified and fluorescence detection is accomplished using Cy5 filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Human Parainfluenza Viruses (HPIVs) Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Human Parainfluenza Viruses (HPIVs) Detection Kit v1 detects and parainfluenza virus RNA (genotypes 1, 2, 3 and 4 in human biological samples such as serum or plasma, urine, respiratory samples, BOS, tissue, swab, nasopharyngeal specimens. A region from parainfluenza genome is amplified and fluorescence detection is achieved in 2 different wells. Parainfluenza 1 is detected with FAM filter and parainfluenza 3 is detected with Texas Red filter in the 1st well. Parainfluenza 2 is detected with HEX filter and parainfluenza 4 is detected with Cy5 filter in the 2nd well.
An internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter in the 1st well and with FAM filter in the 2nd well. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Respiratory Syncytial Virus Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Respiratory Syncytial Virus Detection Kit v1 detects respiratory syncytial virus (RSV) RNA in human biological samples such as serum, plasma, urine, respiratory samples, BOS, tissue, swab, encompassing all RSV genotypes. A region from RSV genome is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Human Metapneumovirus (hMPV) Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Human Metapneumovirus (hMPV) Detection Kit v1 detects human metapneumovirus (hMPV) RNA including all subtypes in human serum or plasma, urine, BOS, stool samples, tissue, swap specimens, respiratory samples and nasopharyngeal specimens. A region of hMPV nucleoprotein gene is amplified and detected with HEX filter.
An internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added during nucleic acid extraction step or PCR step.
Bosphore Rhinovirus Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Rhinovirus Detection Kit v1 detects rhinovirus RNA including all subtypes in human respiratory samples such as nasopharyngeal swab specimens, sputum, nasopharyngeal aspirate, bronchoalveolar lavage and tissue samples. A region of rhinovirus genome is amplified and detected with HEX filter.
The internal control has been integrated into the kit in order to check nucleic acid extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during nucleic acid extraction step or PCR step.
Bosphore Parechovirus Detection Kit v1 detects parechovirus RNA in human biological samples. Parechovirus RNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the CY5 filter. The internal control is added during PCR step.
Bosphore Haemophilus influenzae Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Haemophilus influenzae Detection Kit v1 detects Haemophilus influenzae DNA in human biological samples. A specific region in Haemophilus influenzae genome is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore K. pneumoniae Detection Kit v1 detects Klebsiella pneumoniae DNA in human biological samples. DNA of pathogen is amplified and fluorescence detection is accomplished using FAM filter.
An internal control has been integrated into the kit to check PCR inhibition. The internal control is added during the PCR step and its amplification data is detected with the HEX filter.
Bosphore L. pneumophila Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore L. pneumophila Detection Kit v1 detects Legionella pneumophila DNA in human biological samples, encompassing all the genotypes. A specific region in Legionella pneumophila genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Moraxella catarrhalis Detection Kit v1 detects Moraxella catarrhalis DNA in human biological samples. Moraxella catarrhalis DNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the CY5 filter. The internal control is added during PCR step.
Bosphore Mycoplasma pneumoniae Detection Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore M. pneumoniae Detection Kit v1 detects M. pneumoniae DNA in human biological samples, encompassing all the genotypes. A region within the 16S ribosomal RNA gene is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the FAM filter. The internal control is added in PCR step.
Bosphore Streptococcus pneumoniae Detection Kit v1 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive.
Bosphore S.pneumoniae Detection Kit v1 detects Streptococcus pneumoniae DNA in human biological samples. Streptococcus pneumoniae DNA is amplified and fluorescence detection is accomplished using HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control is added during PCR step.
Bosphore Pneumocystis jirovecii Detection Kit v1 is, a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Pneumocystis jirovecii Detection Kit v1 detects Pneumocystis jirovecii DNA in human biological samples, encompassing all the genotypes. A specific region partially encoding large subunit ribosomal RNA in Pneumocystis jirovecii genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or PCR step.
Bosphore Chlamydia pneumoniae Detection Kit v1 detects Chlamydia pneumoniae DNA in human biological samples, naspharyngeal swab, nasopharyngeal aspirate, tracheal aspirate, bronchoalveolar lavage, sputum and culture. Chlamydia pneumoniae DNA is amplified and fluorescence detection is accomplished using FAM filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the HEX filter. The internal control is added during PCR step.
Bosphore Bordetella pertussis Detection Kit v1 detects Bordetella pertussis DNA including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nose swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. The analytic sensitivity of the kit is 1 copy/reaction. IS481 region in Bordetella pertussis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
The internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application errors. The amplification data of the internal control is detected with the Texas Red filter. The internal control can be added either during DNA extraction or PCR step.
