Adulteration is done by adding foreign or cheaper substances to foods or by replacing valuable ingredients. One of the most common examples of adulteration is the adulteration of milk from cheaper species such as cows with high economic value, such as water buffalo milk.
With the Bosphore Milk Species Identification Real-Time PCR Kits produced by Anatolia, it is possible to detect the presence of bovine, water buffalo, goat and sheep DNA in milk and dairy product samples.
Dairy Species Identification
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Processed and unprocessed food |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Dairy Species Identification Kits
Bosphore A1-A2 Genotyping Kit v1 detects A1 and A2 caseins from dairy and dairy product samples. Fluorescence detection is performed using FAM and HEX filters. A1 casein amplification is screened using FAM filter, and A2 is screened using HEX filter.
|
Component |
FAM (Gene) |
HEX (Gene) |
Cy5 |
|
PCR Master Mix |
A1 Casein (CSN2) |
A2 Casein (CSN2) |
Internal Control |
Internal Control has been integrated into the kit to check PCR inhibition or application problems. The amplification data of Internal Control is detected with the Cy5 filter. Internal Control can be added during the PCR step.
Bosphore Species Identification Kit Bovine v2 detects the presence of bovine species’ DNA in milk and dairy product samples by Real-Time PCR method. Internal Control DNA is detected using the HEX filter, and bovine DNA is detected through the Cy5 filter. The sensitivity/detection limit of the kit is 1%.
Bosphore Species Identification Kit Water Buffalo v2 detects the presence of water buffalo DNA in milk and dairy product samples by Real-Time PCR method. Internal Control DNA is detected using the HEX filter, and water buffalo DNA is detected through the Cy5 filter. The sensitivity/detection limit of the kit is 1%.
Bosphore Species Identification Kit Goat v2 detects the presence of goat DNA in milk and dairy product samples by Real-Time PCR method. Internal Control DNA is detected using the HEX filter, and goat DNA is detected through the Cy5 filter. The sensitivity/detection limit of the kit is 1%.
Bosphore Species Identification Kit Sheep v2 detects the presence of sheep DNA in milk and dairy product samples by Real-Time PCR method. Internal Control DNA is detected using the HEX filter, and sheep DNA is detected through the Cy5 filter. The sensitivity/detection limit of the kit is 1%.
