Real-Time PCR Kits
Adenovirus
Human adenoviruses (HAdVs) are associated with acute respiratory disease (ARD), conjunctivitis, genitourinary infections, and gastroenteritis. Adenovirus is a very common infection and responsible for between 2% and 5% of all respiratory infections. It usually occurs during childhood but all ages are susceptible. In winter, infection with type 4 or 7 causes recognizable illness in military recruits, with about 25% requiring hospitalization for fever and lower respiratory tract disease.
AdV are usually transmitted via the fecal-oral route and the respiratory route. Close contact with infected people such as touching or shaking hands, inhaling the air by coughing and sneezing, touching an object or surface with adenoviruses on it then touching your mouth, nose, or eyes before washing your hands can cause the viral transmission.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Serum, plasma, feces, cerebrospinal fluid |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Adenovirus Kits
Bosphore Adenovirus Quantification Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Hexon gene of Adenovirus from human serum, plasma, stool, and cerebrospinal fluid samples. Fluorescence detection is performed using FAM and HEX filters. Adenovirus DNA is amplified and fluorescence detection is performed using the FAM filter.
|
Component |
FAM |
HEX |
|
PCR Master Mix |
Adenovirus |
Internal Control |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR.
Bosphore EBV – Adenovirus Quantification Kit has been designed to detect, discriminate and quantitate Epstein – Barr virus (EBV / human herpes virus- 4 / HHV-4) and adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat / nasal / dermal / eye / genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage. Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in two PCR tubes.
An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.
| FAM | HEX | Cy5 | |
| PCR Master Mix 1 | Adenovirus (ADV) | Epstein – Barr virus (EBV) | Internal Control |
