Real-Time PCR Kits
Joint Infections Pathogens Panel
Joint infections arise from bacterial or fungal invasion of the joint space and may lead to serious complications such as functional loss and chronic pain if not diagnosed in a timely manner. These infections, including those associated with prosthetic joints, have complex and varied etiologies that require rapid and accurate identification to support appropriate treatment decisions. Bosphore Joint Infections Pathogens Kits enable the simultaneous Real-Time PCR–based detection of common bacterial and fungal pathogens in human synovial fluid samples; selected kits also allow concurrent detection of clinically relevant antibiotic resistance genes, providing comprehensive and reliable diagnostic support for clinical laboratories.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Human synovial fluid samples |
| Shelf Life | 18 Months |
| Channels |
FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Joint Infections Pathogens Panel Kits
Bosphore Joint Infection Panel Kit v2 is a Real-Time PCR kit has designed to detects and characterizes Streptococcus agalactiae, Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis, Klebsiella pneumoniae, Peptostreptococcus anaerobius, Serratia marcescens, Enterococcus faecium, Clostridium perfringens, Peptoniphilus spp., Streptococcus pyogenes, Citrobacter spp., Candida albicans, Morganella morganii, Enterobacter cloacae, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Parvimonas micra, Escherichia coli, Streptococcus spp., Haemophilus influenzae, Proteus spp., Klebsiella aerogenes (E. aerogenes), Salmonella spp., Bacteroides fragilis, Cutibacterium avidum, Staphylococcus lugdunensis, Anaerococcus prevotii/vaginalis, Kingella kingae, Cutibacterium granulosum, Finegoldia magna, and Candida spp. in human synovial fluid samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
| PCR Master Mix 1 | S. agalactiae (cfB) |
S. aureus (nucA) |
S. pneumoniae (lytA) |
E. faecalis (DDL) |
| PCR Master Mix 2 | K. pneumoniae (Hemolysin) |
P. anaerobius (16S rRNA) |
S. marcescens (16S rRNA/ ITS/23S rRNA) |
E. faecium (DDL) |
| PCR Master Mix 3 | C. perfringens (16S rRNA) |
Peptoniphilus spp. (16S rRNA) |
S. pyogenes (speB) |
Citrobacter spp. (phage resistance protein/lacZ) |
| PCR Master Mix 4 | C. albicans (ITS2 region) |
M. morganii (23S rRNA) |
E. cloacae (rpoB) |
P. aeruginosa (16S rRNA) |
| PCR Master Mix 5 | N. gonorrhoeae (Porin) |
P. micra (rpoB) |
E. coli (uidA) |
Streptococcus spp. (16S rRNA/ITS/23S rRNA) |
| PCR Master Mix 6 | H. influenzae (OMP P6) |
Proteus spp. (atpD) |
K. aerogenes (E. aerogenes) (gyrB) |
Salmonella spp. (ttrb) |
| PCR Master Mix 7 | B. fragilis (16S rRNA) |
C. avidum (23S rRNA) |
S. lugdunensis (fbl) |
A. prevotii/vaginalis (16S rRNA) |
| PCR Master Mix 8 | K. kingae (mdh) |
C. granulosum (16S rRNA) |
F. magna (16S rRNA) |
Internal Control |
| PCR Master Mix 9 | – | – | Candida spp. (ITS2 region) |
– |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Cy5 filter for PCR Master Mix 8. The internal control can be added either during DNA extraction or the PCR step.
