Real-Time PCR Kits
Gastroenteritis Panel
Gastrointestinal tract infections (gastroenteritis) include bacterial, viral and protozoan pathogens that cause inflammation of the mucous membranes of the gastrointestinal tract. They rank as the second highest rates of mortality and morbidity following pneumonia, especially in children under 5 years of age in developing countries. Apart from the serious consequences of gastroenteritis infections, the disease is a burden on the health services in developed countries. Therefore, rapid and accurate diagnosis is very important worldwide.
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Stool samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
Gastroenteritis Panel Kits
Bosphore Gastroenteritis Panel Kit v4 detects Sapovirus (I, II, IV, V), Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, Adenovirus, Clostridium difficile toxin A/B, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli VTEC (E. coli STEC (STX1/STX2)), Escherichia coli EIEC/Shigella, Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., Giardia lamblia, Escherichia coli EPEC, Escherichia coli ETEC lt/st, Cyclospora cayetanensis, Escherichia coli EAEC, Escherichia coli STEC O157:H7, Plesiomonas shigelloides, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus from stool samples.
Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.
| FAM | HEX | Cy5 | Texas RED | |
| Tube 1 | Sapovirus (I, II, IV, V) | Astrovirus | Rotavirus A | Internal Control |
| Tube 2 | Norovirus GI | Norovirus GII | Adenovirus | Internal Control |
| Tube 3 | Clostridium difficile toxin A/B | Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari) |
Salmonella spp. | Internal Control |
| Tube 4 | E. coli (VTEC) (E. coli STEC (STX1/STX2)) | E. coli (EIEC)/ Shigella spp. | Yersinia enterocolitica | Internal Control |
| Tube 5 | Entamoeba histolytica | Cryptosporidium spp. | Giardia lamblia (Giardia duodenalis) | Internal Control |
| Tube 6 | E. coli (EPEC) | E. coli (ETEC lt/st) | Cyclospora cayetanensis | Internal Control |
| Tube 7 | E. coli (EAEC) | E. coli (STEC O157:H7) | Plesiomonas shigelloides | Internal Control |
| Tube 8 | Vibrio cholerae | Vibrio parahaemolyticus | Vibrio vulnificus | Internal Control |
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, and application mistakes. The internal control can be added either during DNA extraction or PCR step. The kit also includes positive controls.
Bosphore Gastroenteritis Panel Kit v3 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, Adenovirus, Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli VTEC (E. coli STEC (STX1/STX2)), Escherichia coli (EIEC)/Shigella spp., Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples.
Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM
(Gene) |
HEX
(Gene) |
Texas RED
(Gene) |
Cy5
(Gene) |
| PCR Master Mix 1 | – | Astrovirus
(orf1ab gene) |
Internal Control | Rotavirus A
(nsp3 gene) |
| PCR Master Mix 2 | Norovirus GI
(nonstructural polyprotein gene) |
Norovirus GII
(nonstructural polyprotein gene) |
Internal Control | Adenovirus
(hexon gene) |
| PCR Master Mix 3 | Clostridium difficile (tcdA/tcdB genes) | Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari)(16S rRNA gene) |
Internal Control | Salmonella spp.
(ttrb gene) |
| PCR Master Mix 4 | E. coli (VTEC) (E. coli STEC (STX1/STX2))
(stx-1/stx-2 genes) |
E. coli (EIEC)/ Shigella spp.
(ipaH gene) |
Internal Control | Yersinia enterocolitica
(ail gene) |
| PCR Master Mix 5 | Entamoeba histolytica
(ITS2 gene) |
Cryptosporidium spp.
(COWP gene) |
Internal Control | Giardia duodenalis
(gdh gene) |
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems.
The internal control can be added either during DNA/RNA extraction or PCR step. The fluorescence detection of the internal control is accomplished using the Texas RED filters.
Bosphore Gastroenteritis Panel Kit v2 has been designed to detect astrovirus, rotavirus, norovirus Genotype I, norovirus Genotype II, adenovirus, Clostridium difficile, Campylobacter spp., Salmonella spp., enteroinvasive E.coli (EIEC) and Shigella spp., verotoxigenic E.coli (VTEC) and Yersinia enterocolitica extracted from the following human biological samples including stool samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | – | Astrovirus | Rotavirus | Internal Control |
| Tube 2 | Norovirus GI | Norovirus GII | Adenovirus | Internal Control |
| Tube 3 | C. difficile | Campylobacter spp. | Salmonella spp. | Internal Control |
| Tube 4 | E.coli (VTEC) | EIEC/Shigella spp. | Y. enterocolitica | Internal Control |
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.
Bosphore Gastroenteritis Panel Kit v1 has been designed to detect astrovirus, rotavirus, norovirus Genotype I, norovirus Genotype II, adenovirus, Clostridium difficile, Campylobacter spp. and Salmonella spp. extracted from the following human biological samples including stool samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | – | Astrovirus | Rotavirus | Internal Control |
| Tube 2 | Norovirus GI | Norovirus GII | Adenovirus | Internal Control |
| Tube 3 | Clostridium difficile | Campylobacter spp. | Salmonella spp. | Internal Control |
Bosphore Bacterial GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli (EIEC)/Shigella spp., Escherichia coli (VTEC) (E. coli STEC (STX1/STX2)), and Yersinia enterocolitica from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Bacterial DNA is amplified, and fluorescence detection is performed using the FAM, HEX, and Cy5 filters.
| FAM | HEX | Texas RED | Cy5 | |
| PCR Master Mix 1 | Clostridium difficile toxin A/B | Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari) |
Internal Control | Salmonella spp. |
| PCR Master Mix 2 | Escherichia coli (VTEC) (E. coli STEC (STX1/STX2)) |
Escherichia coli (EIEC) / Shigella spp. | Internal Control | Yersinia enterocolitica |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Parasitic GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Ascaris spp., Taenia spp., Enterobius vermicularis (Oxyuris), Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM (Gene) |
HEX (Gene) |
Texas RED (Gene) |
Cy5 (Gene) |
| PCR Master Mix 1 | Ascaris spp.
(ITS-1 gene) |
Taenia spp.
(ITS1 & 5.8S-rRNA gene) |
Internal Control | Enterobius vermicularis (Oxyuris)
(COX1 gene) |
| PCR Master Mix 2 | Entamoeba histolytica
(5.8S rRNA-ITS2) |
Cryptosporidium spp.
(18S-rRNA) |
Internal Control | Giardia duodenalis
(18S-rRNA) |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Parasitic GI Panel Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM (Gene) | HEX (Gene) | Texas RED (Gene) | Cy5 (Gene) |
| PCR Master Mix 1 | Entamoeba histolytica
(5.8S rRNA-ITS2) |
Cryptosporidium spp.
(18S-rRNA) |
Internal Control | Giardia duodenalis
(18S-rRNA) |
Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.
Bosphore Bacterial and Parasitic GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects the genomic materials of Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli (VTEC) (E. coli STEC (STX1/STX2)), Escherichia coli (EIEC)/Shigella spp., Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 | Clostridium difficile
|
Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari) | Internal Control | Salmonella spp. |
| PCR Master Mix 2 | E. coli (VTEC) (E. coli STEC (STX1/STX2)) | E. coli (EIEC)/ Shigella spp. | Internal Control | Yersinia enterocolitica |
| PCR Master Mix 3 | Entamoeba histolytica | Cryptosporidium spp. | Internal Control | Giardia duodenalis |
Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Texas RED filters. Internal Control can be added either during DNA extraction or the PCR step.
Bosphore Viral GI Panel Kit v1 is an in vitro diagnostic Real-Time PCR kit that detects and characterizes the NSP3 gene of Rotavirus and the ORF1-ORF2 junction region genes of Norovirus GI and Norovirus GII in human stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.
In PCR Master Mix 1, Rotavirus RNA is amplified, and fluorescence detection is performed using the Cy5 filter.
In PCR Master Mix 2, Norovirus GI RNA is amplified, and fluorescence detection is performed using the FAM filter, Norovirus GII RNA is amplified, and fluorescence detection is performed using the HEX filter.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 | – | – | Internal Control | Rotavirus |
| PCR Master Mix 2 | Norovirus GI | Norovirus GII | Internal Control | – |
Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with Texas RED filters. The internal control can be added either during RNA extraction or the PCR step.
Bosphore Viral GI Panel Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the genomic materials of Sapovirus (I, II, IV, V), Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, and Adenovirus from stool samples. Viral DNA/RNA is amplified, and fluorescence detection is performed using the FAM, HEX, and Cy5 filters.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 | Sapovirus
(I, II, IV, V) |
Astrovirus | Internal Control | Rotavirus A |
| PCR Master Mix 2 | Norovirus GI | Norovirus GII | Internal Control | Adenovirus |
Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA/RNA extraction or the PCR step.
