Meningitis & CNS Pathogens

Bosphore Bacterial Meningitis Panel Kit v2 detects and characterizes Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae, Escherichia coli K1, and Cryptococcus gattii/Cryptococcus neoformans in human biological samples including serum, plasma, tissue/biopsy samples and CSF (gold standard).

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.

Bosphore Bacterial Meningitis Panel Kit v3 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the omp6 gene of Haemophilus influenzae, ctrA gene of Neisseria meningitidis and lytA gene of Streptococcus pneumoniae from whole blood, serum, plasma, tissue/biopsy samples, and CSF* samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Haemophilus influenzae DNA is amplified and fluorescence detection is performed using the FAM filter, Neisseria meningitidis DNA is amplified and fluorescence detection is performed using the HEX filter, and Streptococcus pneumoniae DNA is amplified and fluorescence detection is performed using the Texas RED filter.

* CSF samples are the “gold standard” for the diagnosis of meningitis.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step.

Bosphore Neonatal meningitis Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the uidA gene of Escherichia coli, cAMP gene of Streptococcus agalactiae (Group B), and hly gene of Listeria monocytogenes from serum, plasma, and CSF samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Escherichia coli DNA is amplified and fluorescence detection is performed using the FAM filter, Streptococcus agalactiae (Group B) DNA is amplified and fluorescence detection is performed using the HEX filter and Listeria monocytogenes DNA is amplified and fluorescence detection is performed using the Cy5 filter.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filter. The internal control can be added either during DNA extraction or the PCR step.

Bosphore Neonatal Meningitis Panel Kit v2 detects and characterizes Escherichia coli, Streptococcus agalactiae (Group B), Listeria monocytogenes and Streptococcus pneumoniae in human biological samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters. In the first PCR tube with PCR Master Mix 1, Escherichia coli DNA is amplified and fluorescence detection is accomplished using the FAM filter, Streptococcus agalactiae (Group B) DNA is amplified and fluorescence detection is accomplished using the HEX filter and Listeria monocytogenes DNA is amplified and fluorescence detection is accomplished using the Cy5 filter. In the second PCR tube with PCR Master Mix 2, Streptococcus pneumoniae DNA is amplified and fluorescence detection is accomplished using the HEX filter.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition or application problems. The internal control can be added either during DNA extraction or PCR step.

Bosphore Viral Meningitis Panel Kit v2 detects and characterizes herpes simplex virus 1, herpes simplex virus 2, varicella-zoster virus, enterovirus (Coxsackie A and B, echovirus, poliovirus and enterovirus 68-71), parechovirus and mumps DNA and RNA in human biological samples including serum, plasma, tissue/biopsy samples and CSF (gold standard) in two PCR tubes. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors in the samples. The internal control can be added either during DNA/RNA extraction or PCR step.

Bosphore Viral Meningitis Panel Kit v3 detects and characterizes herpes simplex virus 1, herpes simplex virus 2, varicella-zoster virus, enterovirus (coxsackie A and B, echovirus, poliovirus and enterovirus 68 – 71) and parechovirus DNA and RNA in human biological samples including serum, plasma, tissue/biopsy samples and CSF (gold standard) in two PCR tubes. Fluorescence detection is accomplished by using FAM, HEX, Texas RED and Cy5 filters.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application errors in the samples. Internal control can be added either during DNA/RNA extraction or PCR step.

Bosphore CNS Screening Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the pathogens listed below from tissue/biopsy and CSF samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters for PCR Master Mix 1, 2, 3, and PCR Master Mix 4; Cy5 filters for PCR Master Mix 5 and PCR Master Mix 6. The internal control can be added during DNA/RNA extraction or PCR step.

Bosphore CNS Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects the gene regions of the pathogens listed below from human biological samples including serum, plasma, tissue, and CSF. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filter in all PCR Master Mixes. The internal control can be added either during DNA/RNA extraction or the PCR step.

Bosphore CNS Mini Panel Kit v2 is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore CNS Mini Panel Kit v2 detects and characterizes herpes simplex virus 1, herpes simplex virus 2, varicella-zoster virus, parvovirus B19, human herpes virus 6 (HHV-6), human herpes virus 7 (HHV-7), enterovirus and parechovirus DNA in human biological samples. Fluorescense detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

In the first PCR tube with PCR Master Mix 1, herpes simplex virus 1 genome is amplified and fluorescence detection is accomplished using the FAM filter, herpes simplex virus 2 genome is amplified and fluorescence detection is accomplished using the Cy5 filter, varicella-zoster virus genome is amplified and fluorescence detection is accomplished using HEX filter.

In the second PCR tube with PCR Master Mix 2, human herpes virus 6 (HHV-6) genome is amplified and fluorescence detection is accomplished using FAM filter, parvovirus B19 genome is amplified and fluorescence detection is accomplished using HEX filter, human herpes virus 7 (HHV-7) genome is amplified and fluorescence detection is accomplished using Cy5 filter.

In the third PCR tube with PCR Master Mix 3, enterovirus is amplified and fluorescence detection is accomplished using the FAM filter and parechovirus is amplified and fluorescence detection is accomplished using the HEX filter.

An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Rash-Fever Panel Kit v1 detects and characterizes parvovirus B19, human herpes virus 6 (HHV-6), human herpes virus 7 (HHV-7), enterovirus and parechovirus in human biological samples including tissue/biopsy specimen, throat swab, sputum, serum, plasma, and CSF. The test is accomplished in two PCR tubes. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control can be added either during nucleic acid extraction or PCR step.

Bosphore Vesicular Rash Panel Kit is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Vesicular Rash Panel Kit detects and characterizes herpes simplex virus 1, herpes simplex virus 2 and varicella-zoster virus DNA in human biological samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters. Herpes simplex virus 1 is amplified and fluorescence detection is accomplished using the FAM filter, herpes simplex virus 2 is amplified and fluorescence detection is accomplished using the Cy5 filter and varicella-zoster virus is amplified and fluorescence detection is accomplished using the HEX filter.

An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Viral Infections in the Immunosuppressed Panel Kit is an in vitro diagnostic Real-Time Polymerase Chain Reaction (qPCR) kit intended for qualitative detection of Adenovirus, EBV (Epstein-Barr Virus), and CMV (Cytomegalovirus) viral DNA extracted from human serum, plasma, bone marrow, amniotic fluid, urine, cerebrospinal fluid, bronchoalveolar lavage, whole blood and eye swab in individuals suspected of being infected with related pathogens by health authorities.

Bosphore Viral Infections in the Immunosuppressed Panel Kit detects three pathogens; Adenovirus, EBV (Epstein-Barr Virus), and CMV (Cytomegalovirus) in a single tube: the conserved regions of DNA polymerase gene of CMV, Hexon gene of Adenovirus, and IR1 region of Epstein-Barr virus are used as target sites to discriminate pathogens specifically. Fluorescence detection is accomplished using FAM, HEX, Texas RED, and Cy5 filters.

An internal control is included in the kit to control DNA extraction, PCR inhibition, and application mistakes. The internal control is a synthetic DNA molecule.

Bosphore Viral Infections in the Immunosuppressed Maxi Panel Bundle Kit detects and characterizes JCV DNA, BKV DNA, CMV DNA, EBV DNA and adenovirus DNA human biological samples; serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate, bronchoalveolar lavage. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application errors. Amplification data of the internal control is detected with Cy5 or Texas RED filter as indicated in the table shown above. Internal control can be added either during nucleic acid extraction or PCR step.

Bosphore VIP Quantification Bundle Kit has been designed to detect, discriminate and quantitate cytomegalovirus (CMV), Epstein-Barr virus (EBV/human herpes virus-4/HHV-4) and adenovirus (ADV) DNA in human biological samples including serum, plasma, bone marrow, amniotic fluid, urine, biopsy material, cerebrospinal fluid, throat/nasal/dermal/eye/genital swab, nasopharyngeal swab, nasopharyngeal aspirate and bronchoalveolar lavage.

Fluorescence detection is accomplished using the FAM, HEX and Cy5 filters in two PCR tubes.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.