Gastroenteritis Pathogens

Bosphore Helicobacter pylori Genotyping Kit v1 is an in vitro diagnostic Real-Time PCR kit that detects and characterizes clarithromycin resistance point mutations A2142G, A2142C, and A2143G in the 23S RNA of Helicobacter pylori from stool, gastric tissue biopsy, biopsy specimens embedded in paraffin specimens. Fluorescence detection is performed using FAM, HEX, and Cy5 filters.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or the PCR step.

Bosphore C. difficile Detection Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the toxin A (tcdA) and toxin B (tcdB) genes of Clostridium difficile (C. difficile) from human stool samples. Fluorescence detection is performed using FAM, HEX, and Cy5 filters. 

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Cy5 filter. The internal control can be added either during DNA extraction or the PCR step. 

Bosphore Gastroenteritis Panel Kit v2 has been designed to detect astrovirus, rotavirus, norovirus Genotype I, norovirus Genotype II, adenovirus, Clostridium difficile, Campylobacter spp., Salmonella spp., enteroinvasive E.coli (EIEC) and Shigella spp., verotoxigenic E.coli (VTEC) and Yersinia enterocolitica extracted from the following human biological samples including stool samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.

An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. The internal control can be added either during DNA extraction or PCR step.

Bosphore Gastroenteritis Panel Kit v3 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, Adenovirus, Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli VTEC (E. coli STEC (STX1/STX2)), Escherichia coli (EIEC)/Shigella spp., Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples.

Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems.

The internal control can be added either during DNA/RNA extraction or PCR step. The fluorescence detection of the internal control is accomplished using the Texas RED filters.

Bosphore Gastroenteritis Panel Kit v4 detects Sapovirus (I, II, IV, V), Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, Adenovirus, Clostridium difficile toxin A/B, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli VTEC (E. coli STEC (STX1/STX2)), Escherichia coli EIEC/Shigella, Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., Giardia lamblia, Escherichia coli EPEC, Escherichia coli ETEC lt/st, Cyclospora cayetanensis, Escherichia coli EAEC, Escherichia coli STEC O157:H7, Plesiomonas shigelloides, Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus from stool samples.

Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, and application mistakes. The internal control can be added either during DNA extraction or PCR step. The kit also includes positive controls.

Bosphore Bacterial GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli (EIEC)/Shigella spp., Escherichia coli (VTEC) (E. coli STEC (STX1/STX2)), and Yersinia enterocolitica from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Bacterial DNA is amplified, and fluorescence detection is performed using the FAM, HEX, and Cy5 filters.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.

Bosphore Parasitic GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Ascaris spp., Taenia spp., Enterobius vermicularis (Oxyuris), Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. 

 Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.  

Bosphore Parasitic GI Panel Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

 Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA extraction or the PCR step.  

Bosphore Parasitic GI Panel Kit v3 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Ascaris spp., Taenia spp., Enterobius vermicularis (Oxyuris), Entamoeba histolytica, Cryptosporidium spp., Strongyloides stercoralis, and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Texas RED filter of PCR Master Mix 1. The internal control can be added either during DNA extraction or the PCR step.

This version of the kit is fully compatible with UNIO 448 System, which performs automated magnetic bead–based nucleic acid extraction, PCR setup, amplification, and fluorescence detection in a closed and contamination-controlled environment.

Bosphore Parasitic GI Panel Kit v4 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Blastocystis hominis, Dientamoeba fragilis, Cyclospora cayetanensis, Entamoeba histolytica, Cryptosporidium spp., and Giardia lamblia from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Texas RED filter of PCR Master Mix 1 and PCR Master Mix 2. The internal control can be added either during DNA extraction or the PCR step.

Bosphore Parasitic GI Panel Kit v6 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes Ascaris spp., Taenia spp., Enterobius vermicularis (Oxyuris), Microsporidia (Enterocytozoon bieneusi, Encephalitozoon intestinalis, Encephalitozoon hellem, Encephalitozoon cuniculi), Entamoeba histolytica, Cryptosporidium spp., Strongyloides stercoralis, and Giardia duodenalis from stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, Cy5 and Quasar 705 filters.

Internal control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of internal control is detected with the Texas RED filter of PCR Master Mix 1 and Quasar 705 filter of PCR Master Mix 2. The internal control can be added either during DNA extraction or the PCR step. 

Bosphore Bacterial and Parasitic GI Panel Kit v1 is a Real-Time PCR kit for in vitro diagnostics that detects the genomic materials of Clostridium difficile, Campylobacter (C. jejuni, C. upsaliensis, C. coli, C. lari), Salmonella spp., Escherichia coli (VTEC) (E. coli STEC (STX1/STX2)), Escherichia coli (EIEC)/Shigella spp., Yersinia enterocolitica, Entamoeba histolytica, Cryptosporidium spp., and Giardia duodenalis from stool samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED, and Cy5 filters.   

Internal Control has been integrated into the kit to check DNA extraction, PCR inhibition, or application problems. The amplification data of Internal Control is detected with the Texas RED filters. Internal Control can be added either during DNA extraction or the PCR step.  

Bosphore Viral GI Panel Kit v1 is an in vitro diagnostic Real-Time PCR kit that detects and characterizes the NSP3 gene of Rotavirus and the ORF1-ORF2 junction region genes of Norovirus GI and Norovirus GII in human stool samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters.  

In PCR Master Mix 1, Rotavirus RNA is amplified, and fluorescence detection is performed using the Cy5 filter.  

In PCR Master Mix 2, Norovirus GI RNA is amplified, and fluorescence detection is performed using the FAM filter, Norovirus GII RNA is amplified, and fluorescence detection is performed using the HEX filter.   

 Internal control has been integrated into the kit to check RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with Texas RED filters. The internal control can be added either during RNA extraction or the PCR step. 

Bosphore Viral GI Panel Kit v2 is a Real-Time PCR kit for in vitro diagnostics that detects and characterizes the genomic materials of Sapovirus (I, II, IV, V), Astrovirus, Rotavirus A, Norovirus GI, Norovirus GII, and Adenovirus from stool samples. Viral DNA/RNA is amplified, and fluorescence detection is performed using the FAM, HEX, and Cy5 filters.   

 Internal control has been integrated into the kit to check DNA/RNA extraction, PCR inhibition, or application problems. The amplification data of the internal control is detected with the Texas RED filters. The internal control can be added either during DNA/RNA extraction or the PCR step.