Real-Time PCR Kits
GMO Detection and Screening
GMO (genetically modified organism) is an organism whose genetic material has been modified through genetic engineering techniques known as ‘recombinant technology’ which consists of creating a kind of artificial DNA by joining different sequences and then the introduction of this related DNA into an existing organismal DNA for different purposes such as producing genetically modified (GM) foods. Even though animal products have been genetically altered, typically modified foods are mostly transgenic plant products, which are named GM plants (GMPs), such as soybean, maize, canola and cottonseed oil and etc.
There are several common pros and cons of GMOs considered in our life. The probable advantages of GMOs are; agricultural-increased yield, environmental-reduced use of pesticides, herbicides, and fuel, improved nutritional quality of foods and disease prevention foods that working like vaccines that are safe to eat. On the other hand, there are disadvantages of GMOs which may be; exposure to possible allergens and toxins, harm to environment, antibiotic resistance, and the spread of introduced genes to non-target plants by outcrossing and pollen drift.
Features
Workflow
Technical Specifications
The channels and sample types indicated in this table may vary depending on the kits (singleplex/multiplex). Detailed information on the associated kits can be found below.
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Types | Processed and unprocessed food, feed, seed samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX, Texas Red, Cy5 |
| Shipping / Storage | (-90°C)-(-20°C) / -20°C |
GMO Detection and Screening Kits
Bosphore GMO Detection Kit S35 v1, detects the 35S promoter from cauliflower mosaic virus (CAMV P35S). An internal control has been integrated into the kit in order to check PCR inhibition. 35S promoter from cauliflower mosaic virus is amplified and fluorescence detection is accomplished using the FAM filter, and internal control is amplified and fluorescence detection is accomplished using the Cy5 filter.
Bosphore GMO Detection Kit tNOS v1, detects the NOS terminator from Agrobacterium tumefaciens – (tNOS). An internal control has been integrated into the kit in order to check PCR inhibition. NOS terminator from Agrobacterium tumefaciens is amplified and fluorescence detection is accomplished using the HEX filter. The amplification data of the internal control is detected with the Cy5 filter.
Bosphore GMO Detection Kit FMV v1, detects the 35S promoter from figwort mosaic virus – (FMV). An internal control has been integrated into the kit in order to check PCR inhibition. 35S promoter from figwort mosaic virus (FMV) is amplified and fluorescence detection is accomplished using the Texas Red filter. The amplification data of the internal control is detected with the Cy5 filter.
Bosphore GMO Detection Kit BAR v1, detects the phosphinothricin N-acetyl transferase (BAR) gene from bacterium Streptomyces hygroscopicus. An internal control has been integrated into the kit in order to check PCR inhibition. Phosphinothricin N-acetyl transferase (BAR) gene is amplified and fluorescence detection is accomplished using the FAM filter. The amplification data of the internal control is detected with the Cy5 filter.
Bosphore GMO Screening Kit S35-TNOS-FMV-BAR v1 detects the most common genetic elements such as P35S promoter from Cauliflower Mosaic Virus- CAMV P35S, P35S promoter from Figwort Mozaic Virus- FMV P35S, NOS terminator from Agrobacterium tumefaciens – tNOS and phosphinothricin N-acetyltransferase from bacterium Streptomyces hygroscopicus – BAR in samples from different matrices (product groups) such as processed and unprocessed food, animal feed or seed samples. Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. In the first PCR tube with PCR Master Mix 1, P35S is amplified and fluorescence detection is performed using the FAM filter, tNOS is amplified and fluorescence detection is performed using the HEX filter and FMV is amplified and fluorescence detection is performed using the Texas RED filter. In the second PCR tube with PCR Master Mix 2, BAR is amplified and fluorescence detection is performed using the HEX filter.
| Component | FAM | HEX | Texas RED | Cy5 |
| PCR Master Mix 1 (S35-TNOS- FMV) | S35 | tNOS | FMV | Internal Control |
| PCR Master Mix 2 (BAR) | – | BAR | – | Internal Control |
Internal control has been integrated into the kit to check PCR inhibition or application problems. The amplification data of the internal control is detected with the Cy5 filter in both PCR tubes. The internal control can be added to the PCR step.
Bosphore GMO Screening Kit S35-tNOS-FMV v3, detects the most common genetic elements (Such as 35S promoter from cauliflower mosaic virus – CAMV P35S, 35S promoter from figwort mosaic virus – FMV P35S and NOS terminator from Agrobacterium tumefaciens – tNOS).
An internal control has been integrated into the kit in order to check PCR inhibition. 35S promoter from cauliflower mosaic virus is amplified and fluorescence detection is accomplished using the FAM filter. 35S promoter from figwort mosaic virus (FMV) is amplified and fluorescence detection is accomplished using the Texas Red filter. NOS terminator from Agrobacterium tumefaciens is amplified and fluorescence detection is accomplished using the HEX filter. The amplification data of the internal control is detected with the Cy5 filter.
