Sepsis Panel

IVD CE

Sepsis is a syndrome commonly caused by the inflammatory response of the immune system as a result of bacterial, viral, protozoan or fungal infections, which may result in organ failure and death if progressed. Primary infection causing sepsis can be seen in lungs, brain, urinary tract, skin or abdominal region. Sepsis can lead to severe sepsis, septic shock stages and death due to dysfunction of one or more organs if early diagnosis and treatment is not provided. It is known as the main cause of maternal and neonatal diseases and deaths, especially in low- and middle-income countries.

The organisms that cause sepsis can be exampled as E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. Risk factors for the development of sepsis include early and advanced age and weakened immune system due to cancer, diabetes, major trauma or burns.

Features

Single Tube Master Mix

Internal Control for Each Reaction

Flexible Packaging

Compatibility with Various Systems

Expert Support

Workflow

Technical Specifications

Thermal Protocol Single thermal protocol for all parameters
Sample Typing Eye swab, ocular fluid, and conjunctival specimen
Shelf Life 18 Months
Channels FAM, HEX/VIC/JOE, Texas Red/ROX, Cy5
Shipping / Storage -80°C / -20°C

Sepsis Panel Kits

Bosphore Sepsis Pathogens Panel Kit v1 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

In the first tube with PCR Master Mix 1; Escherichia coli is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecalis is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the second tube with PCR Master Mix 2; Klebsiella pneumoniae is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus spp. is amplified and fluorescence detection is accomplished using the HEX filter and Enterococcus faecium is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the third tube with PCR Master Mix 3; Acinetobacter baumannii virus is amplified and fluorescence detection is accomplished using the FAM filter, Stenotrophomonas maltophilia is amplified and fluorescence detection is accomplished using the HEX filter and Pseudomonas aeruginosa is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the fourth tube with PCR Master Mix 4; Candida albicans is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus epidermidis is amplified and fluorescence detection is accomplished using the HEX filter and Candida glabrata is amplified and fluorescence detection is accomplished using the Cy5 filter.

In the fifth tube with PCR Master Mix 5; Aspergillus spp. is amplified and fluorescence detection is accomplished using the FAM filter, Candida tropicalis is amplified and fluorescence detection is accomplished using the HEX filter and Streptococcus spp. is amplified and fluorescence detection is accomplished using the Cy5 filter.

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Sepsis Pathogens Panel Kit v2 detects and characterizes Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Enterococcus faecalis, Enterococcus faecium Pseudomonas aeruginosa, Haemophilus influenzae (type B), Neisseria meningitidis, Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, Staphylococcus spp. and Streptococcus spp. DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

FAM HEX Texas Red Cy5
Tube 1 E. coli S. aureus Internal Control E. faecalis
Tube 2 K. pneumoniae Staphylococcus spp. Internal Control E. faecium
Tube 3 H. influenzae (type B) S. pneumoniae Internal Control P. aeruginosa
Tube 4 X N. meningitidis Internal Control Streptococcus spp.
Tube 5 X L. monocytogenes Internal Control S. enterica

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Sepsis Pathogens Panel Kit v3 detects and characterizes E. coli, Klebsiella pneumoniae, S. aureus, Staphylococcus epidermidis, Enterococcus faecalis, Enterococcus faecium, Acinetobacter baumannii, Stenotrophomonas maltophilia, Pseudomonas aeruginosa, Candida albicans, Candida glabrata, Candida tropicalis, Aspergillus spp., coagulase-negative Staphylococcus (CoNS), Staphylococcus spp., Streptococcus spp., Haemophilus influenzae (type B), Listeria monocytogenes, Salmonella enterica, Streptococcus pneumoniae, DNA in whole blood and blood culture samples. Fluorescence detection is accomplished by using FAM, HEX, Texas Red and Cy5 filters.

FAM HEX Texas Red Cy5
Tube 1 E. coli S. aureus Internal Control E. faecalis
Tube 2 K. pneumoniae Staphylococcus spp. Internal Control E. faecium
Tube 3 A. baumannii S. maltophilia Internal Control P. aeruginosa
Tube 4 C. albicans S. epidermidis Internal Control C. glabrata
Tube 5 Aspergillus spp. C. tropicalis Internal Control Streptococcus spp.
Tube 6 H. influenzae (type B) S. pneumoniae Internal Control X
Tube 7 X L. monocytogenes Internal Control S. enterica

An internal control has been integrated into the kit in order to check DNA extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with Texas Red filter. The internal control can be added either during DNA extraction or PCR step.

Bosphore Sepsis Pathogens Panel Kit v4 is a Real-Time PCR kit designed to detect and characterize 32 different pathogens and the 10 most common antimicrobial resistance genes.

The target pathogens of the kit includes Gram(-) A. baumannii, Campylobacter spp, E. aerogenes, E. cloacae, E. coli, H. influenzae type b, K. oxytoca, K. pneumoniae, N. meningitidis, P. aeruginosa, P. mirabilis, S. enterica, S. maltophilia, S. marcescens, and Gram(+) E. faecalis, E. faecium, L. monocytogenes, Staphylococcus spp., S. aureus, S. epidermidis, Streptococcus spp., S. agalactiae, S. pneumoniae, S. pyogenes, and also fungi Aspergillus spp., C. albicans, C. glabrata, C. krusei, C. auris, C. tropicalis, C. parapsilosis, Fusarium spp.

The resistance genes targeted by the kit are OXA-48, KPC, NDM, VIM, IMP (carbapenem resistance genes), vanA, vanB (vancomycin resistance genes), MCR-1 (colistin resistance), mecA (+)(methicillin resistance) and lastly ESBL CTX-M (ß-lactam resistance).

Fluorescence detection is performed using FAM, HEX, Texas RED, and Cy5 filters. Internal and positive controls were integrated into the kit.

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