Respiratory infections involve bacterial and viral pathogens affecting the upper or lower respiratory tract, and they are one of the major causes of mortality and morbidity, especially among children. Some respiratory infections may only cause mild symptoms such as common cold while some other may result in more serious cases; accounting for around 10 million deaths per year globally. lnvolvement of different viral and bacterial causative agents, complicates the diagnosis of the primary pathogen.
Respiratory Panel
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Typing | Respiratory samples such as nasopharyngeal swab specimens, sputum, nasopharyngeal aspirate, bronchoalveolar lavage and tissue samples |
| Shelf Life | 18 Months |
| Channels | FAM, HEX/VIC, Texas Red/ROX, Cy5 |
| Shipping / Storage | -80°C / -20°C |
Respiratory Panel Kits
Bosphore SARS-CoV-2 / Respiratory Pathogens Panel Kit v1 detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B , influenza A&B, enterovirus, metapneumovirus, adenovirus, human parainfluenza 1/2/3/4, rhinovirus, Mycoplasma pneumoniae and Legionella pneumophila, in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva.
Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters. SARS-CoV-2 is detected by two regions of the virus (Orf1ab and N gene nCoV specific) from a single channel FAM. RSV A&B is detected by HEX channel. Influenza A&B is detected by Cy5 channel. Enterovirus is detected by FAM channel. Metapneumovirus is detected by HEX channel. Adenovirus is detected by Cy5 channel. Human parainfluenza virus 1/2/3/4 is detected by FAM channel. Rhinovirus is detected by HEX channel. Legionella pneumophila is detected by Texas Red channel. Mycoplasma pneumoniae is detected by Cy5 channel.
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. Internal control is detected by Texas Red channel in Tube 1 and Tube 2.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | SARS-Cov 2 | RSV A&B | Influenza A&B | Internal Control |
| Tube 2 | Enterovirus | Metapneumovirus | Adenovirus | Internal Control |
| Tube 3 | Human Parainfluenza 1/2/3/4 | Rhinovirus | Mycoplasma pneumoniae | Legionella pneumophila |
Bosphore Respiratory Pathogens Panel Kit v1, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Respiratory Pathogens Panel Kit v1 detects and characterizes influenza B, Mycoplasma pneumoniae, Klebsiella pneumoniae, parainfluenza 2, parainfluenza 4, parainfluenza 1, metapneumovirus, enterovirus, influenza A, parainfluenza 3, RSV A/B, bocavirus, rhinovirus, coronavirus 229E, pandemic H1N1 influenza A, seasonal H1N1 influenza A and Salmonella in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, influenza B genome is amplified and fluorescence detection is accomplished using the HEX filter, Mycoplasma pneumoniae genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Klebsiella pneumoniae genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the second PCR tube with PCR Master Mix 2, parainfluenza 2 genome is amplified and fluorescence detection is accomplished using the HEX filter, parainfluenza 4 genome is amplified and fluorescence detection is accomplished using the Cy5 filter and parainfluenza 1 genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the third PCR tube with PCR Master Mix 3, metapneumovirus genome is amplified and fluorescence detection is accomplished using the HEX filter and enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the fourth PCR tube with PCR Master Mix 4, influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, parainfluenza 3 genome is amplified and fluorescence detection is accomplished using the Texas Red filter and RSV A/B genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the fifth PCR tube with PCR Master Mix 5, bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter, rhinovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the sixth PCR tube with PCR Master Mix 6, pandemic H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, seasonal H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas Red filter in PCR Master Mix 1, PCR Master Mix 2, PCR Master Mix 3 and PCR Master Mix 6, it is detected with the Cy5 in PCR Master Mix 4 and PCR Master Mix 5. The internal control can be added either during DNA extraction or PCR step.
Bosphore Respiratory Pathogens Panel Kit v4, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Respiratory Pathogens Panel Kit v4 detects and characterizes influenza B, Mycoplasma pneumoniae, Klebsiella pneumoniae, parainfluenza 2, parainfluenza 4, parainfluenza 1, metapneumovirus, enterovirus, influenza A, parainfluenza 3, RSV A/B, bocavirus, rhinovirus, coronavirus 229E, pandemic H1N1 influenza A, seasonal H1N1 influenza A, Salmonella enterica, adenovirus, Moraxella catarrhalis, Bordetella pertussis, Haemophilus influenzae type B, parechovirus, Staphylococcus aureus, Pneumocystis jiroveci, Streptococcus pneumoniae, Legionella pneumophila, coronavirus OC43 and coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, influenza B genome is amplified and fluorescence detection is accomplished using the HEX filter, Mycoplasma pneumoniae genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Klebsiella pneumoniae genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the second PCR tube with PCR Master Mix 2, parainfluenza 2 genome is amplified and fluorescence detection is accomplished using the HEX filter, parainfluenza 4 genome is amplified and fluorescence detection is accomplished using the Cy5 filter and parainfluenza 1 genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the third PCR tube with PCR Master Mix 3, metapneumovirus genome is amplified and fluorescence detection is accomplished using the HEX filter and enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the fourth PCR tube with PCR Master Mix 4, influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, parainfluenza 3 genome is amplified and fluorescence detection is accomplished using the Texas Red filter and RSV A/B genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the fifth PCR tube with PCR Master Mix 5, bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter, rhinovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the sixth PCR tube with PCR Master Mix 6, pandemic H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, seasonal H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the seventh PCR tube with Master Mix 7, adenovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, Moraxella catarrhalis genome is amplified and fluorescence detection is accomplished using the HEX filter and Bordetella pertussis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the eighth PCR tube with Master Mix 8, Haemophilus influenzae type B is amplified and fluorescence detection is accomplished using the FAM filter, parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the ninth PCR tube with Master Mix 9, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Pneumocystis jiroveci genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the tenth PCR tube with Master Mix 10, Streptococcus pneumoniae genome is amplified and fluorescence detection is accomplished using the HEX filter and Legionella pneumophila genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the eleventh PCR tube with Master Mix 11, coronavirus OC43 is amplified and fluorescence detection is accomplished using the FAM filter, coronavirus NL63 is amplified and fluorescence detection is accomplished using the HEX filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas Red filter in PCR Master Mix 1, PCR Master Mix 2, PCR Master Mix 3, PCR Master Mix 6, Master Mix 7, Master Mix 8 and PCR Master Mix 11, while it is detected with the Cy5 in PCR Master Mix 4, PCR Master Mix 5, PCR Master Mix 9 and PCR Master Mix 10. The internal control can be added either during DNA extraction or PCR step.
Bosphore Respiratory Pathogens Panel Kit v5, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Respiratory Pathogens Panel Kit v5 detects and characterizes influenza B, Mycoplasma pneumoniae, Klebsiella pneumoniae, parainfluenza 2, parainfluenza 4, parainfluenza 1, metapneumovirus, enterovirus, influenza A, parainfluenza 3, RSV A/B, bocavirus, rhinovirus, coronavirus 229E, pandemic H1N1 influenza A, seasonal H1N1 influenza A, influenza C, Salmonella enterica, adenovirus, Moraxella catarrhalis, Bordetella pertussis, Haemophilus influenzae type B, parechovirus, Staphylococcus aureus, Pneumocystis jiroveci, Streptococcus pneumoniae, Legionella pneumophila, coronavirus OC43, coronavirus NL63 and coronavirus HKU1 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
In the first PCR tube with PCR Master Mix 1, influenza B genome is amplified and fluorescence detection is accomplished using the HEX filter, Mycoplasma pneumoniae genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Klebsiella pneumoniae genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the second PCR tube with PCR Master Mix 2, parainfluenza 2 genome is amplified and fluorescence detection is accomplished using the HEX filter, parainfluenza 4 genome is amplified and fluorescence detection is accomplished using the Cy5 filter and parainfluenza 1 genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the third PCR tube with PCR Master Mix 3, metapneumovirus genome is amplified and fluorescence detection is accomplished using the HEX filter and enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter.
In the fourth PCR tube with PCR Master Mix 4, influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, parainfluenza 3 genome is amplified and fluorescence detection is accomplished using the Texas Red filter and RSV A/B genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the fifth PCR tube with PCR Master Mix 5, bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter, rhinovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the sixth PCR tube with PCR Master Mix 6, pandemic H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, seasonal H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the seventh PCR tube with Master Mix 7, adenovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, Moraxella catarrhalis genome is amplified and fluorescence detection is accomplished using the HEX filter and Bordetella pertussis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.
In the eighth PCR tube with Master Mix 8, Haemophilus influenzae type B is amplified and fluorescence detection is accomplished using the FAM filter, parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter.
In the ninth PCR tube with Master Mix 9, influenza C is amplified and fluorescence detection is accomplished using the FAM filter, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Pneumocystis jiroveci genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the tenth PCR tube with Master Mix 10, Streptococcus pneumoniae genome is amplified and fluorescence detection is accomplished using the HEX filter and Legionella pneumophila genome is amplified and fluorescence detection is accomplished using the Texas Red filter.
In the eleventh PCR tube with Master Mix 11, coronavirus OC43 is amplified and fluorescence detection is accomplished using the FAM filter, coronavirus NL63 is amplified and fluorescence detection is accomplished using the HEX filter and coronavirus HKU1 is amplified and fluorescence detection is accomplished using the Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas Red filter in PCR Master Mix 1, PCR Master Mix 2, PCR Master Mix 3, PCR Master Mix 6, Master Mix 7, Master Mix 8 and PCR Master Mix 11, while it is detected with the Cy5 in PCR Master Mix 4, PCR Master Mix 5, PCR Master Mix 9 and PCR Master Mix 10. The internal control can be added either during DNA extraction or PCR step.
Bosphore Respiratory Pathogens Panel Kit v6 detects and characterizes parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, enterovirus, metapneumovirus, Mycoplasma pneumoniae, H. influenza type B, influenza A, influenza B, RSV A/B, adenovirus, parechovirus, bocavirus, rhinovirus, coronavirus 229E, coronavirus OC43, coronavirus NL63, coronavirus HKU1 and pandemic H1N1 influenza A in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
| Tube 2 | Enterovirus | Metapneumovirus | – | Internal Control |
| Tube 3 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
| Tube 4 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
| Tube 5 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
| Tube 6 | H. influenza type B | Influenza B | M. pneumoniae | Internal Control |
| Tube 7 | Pandemic H1N1 | Parechovirus | Adenovirus | Internal Control |
Bosphore Respiratory Pathogens Maxi Panel detects and characterizes adenovirus, bocavirus, Bordetella pertussis, coronavirus 229E, coronavirus OC43, coronavirus NL63, coronavirus HKU1, Chlamydia pneumoniae, enterovirus, Haemophilus influenzae type B, influenza A, influenza B, influenza C, Klebsiella pneumoniae, Legionella pneumophila, Legionella pneumophila / longbeachae, Mycoplasma pneumoniae, Moraxella catarrhalis, metapneumovirus, RSV A/B, pandemic H1N1 influenza A, parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, parechovirus, Pneumocystis jirovecii, rhinovirus, seasonal H1N1 influenza A, Salmonella enterica, Staphylococcus aureus, and Streptococcus pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 – Box 1 | K. pneumoniae | Influenza B | M. pneumoniae | Internal Control |
| Tube 2 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
| Tube 3 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
| Tube 4 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
| Tube 5 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
| Tube 6 – Box 1 | Pandemic H1N1 | Seasonal H1N1 | S. enterica | Internal Control |
| Tube 7 – Box 1 | Adenovirus | M. catarrhalis | B. pertussis | Internal Control |
| Tube 8 – Box 1 | H. influenza type B | Parechovirus | – | Internal Control |
| Tube 9 -Box 1 | Influenza C | S. aureus | Internal Control | P. jirovecii |
| Tube 10 -Box 1 | – | S. pneumoniae | Internal Control | L. pneumophila |
| Tube 11 -Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
| Tube 1 – Box 2 | C. pneumoniae | Internal Control | M. pneumoniae | L. pneumophila / longbeachae |
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
Bosphore Respiratory Pathogens Mini Panel Kit detects and characterizes parainfluenza 1-2-3-4, enterovirus, metapneumovirus, influenza A-B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus 229E-HKU1-NL63-OC43, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae and Mycoplasma pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
| Tube 2 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
| Tube 3 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
| Tube 4 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
| Tube 5 – Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
| Tube 6 – Box 1 | Pandemic H1N1 | Influenza B | Adenovirus | Internal Control |
| Tube 7 – Box 1 | H. influenza type B | Parechovirus | Internal Control | |
| Tube 8 – Box 1 | – | S. aureus | Internal Control | S. pneumoniae |
Bosphore Respiratory Pathogens Mini Panel Bundle Kit detects and characterizes parainfluenza 1, parainfluenza 2, parainfluenza 3, parainfluenza 4, enterovirus, metapneumovirus, influenza A, influenza B, pandemic H1N1, RSV A/B, bocavirus, rhinovirus, coronavirus OC43, coronavirus NL63, coronavirus HKU1, coronavirus 229E, adenovirus, H. influenza type B, parechovirus, Staphylococcus aureus, Streptococcus pneumoniae, Mycoplasma pneumoniae, Legionella pneumophila / longbeachae, Chlamydophila pneumoniae in human respiratory samples including biopsy (tissue) samples, BAL, sputum, tracheal aspirate, nasopharyngeal aspirate, throat swab, nasopharyngeal swab: Flocking swabs and Liquid Amies, Universal Transport Medium, VCM, Sigma Transwab, Copan ESwab, Sigma VCM. Fluorescence detection is accomplished using FAM, HEX, Texas Red and Cy5 filters.
An internal control has been integrated into the kit in order to check nucleic acid extraction, PCR inhibition and application mistakes. Amplification data of the internal control is detected with HEX, Texas Red filter or Cy5 filter as table shown above. Internal control can be added either during nucleic acid extraction or PCR step.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 – Box 1 | Parainfluenza 1 | Parainfluenza 2 | Parainfluenza 4 | Internal Control |
| Tube 2 – Box 1 | Enterovirus | Metapneumovirus | – | Internal Control |
| Tube 3 – Box 1 | Influenza A | RSV A/B | Internal Control | Parainfluenza 3 |
| Tube 4 – Box 1 | Bocavirus | Rhinovirus | Internal Control | Coronavirus 229E |
| Tube 5 – Box 1 | Coronavirus OC43 | Coronavirus NL63 | Coronavirus HKU1 | Internal Control |
| Tube 6 – Box 1 | Pandemic H1N1 | Influenza B | Adenovirus | Internal Control |
| Tube 7 – Box 1 | H. influenza type B | Parechovirus | Internal Control | |
| Tube 8 – Box 1 | – | S. aureus | Internal Control | S. pneumoniae |
| Tube 1 – Box 2 | C. pneumoniae | Internal Control | M. pneumoniae | L. pneumophila / longbeachae |
Bosphore Respiratory Viral Basic Panel Kit detects and characterizes adenovirus, enterovirus, Legionella pneumophila, metapneumovirus, Mycoplasma pneumoniae, rhinovirus, and parainfluenza viruses (1, 2, 3, 4) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using the FAM, HEX, Texas Red and Cy5 filters.
| FAM | HEX | Cy5 | Texas Red | |
| Tube 1 | Enterovirus | Metapneumovirus | Adenovirus | Internal Control |
| Tube 2 | Human Parainfluenza 1/2/3/4 | Rhinovirus | Mycoplasma pneumoniae | Legionella pneumophila |
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
