Bosphore Manual Spin Column Nucleic Acid Extraction Kits are based on the silica membrane column separation method. The kit involves extraction of nucleic acids from biological samples of patients by removing and purifying the nucleic acids from cell components. In this manner, RNA isolation steps include disruption/lysis of the samples, inactivation of nuclease activity, binding of the RNA to the silica membrane, removal of the contaminants and the genomic DNA, and recovery of the nucleic acid respectively. So far, methods generally employed have been considered in two categories; the conventional ‘liquid-phase isolation’ and common ‘solid-phase isolation’ which is highly appreciated in molecular biology field as a result of its practical use.
Manual Spin Column Extraction
Features
Workflow
Technical Specifications
| Thermal Protocol | Single thermal protocol for all parameters |
| Sample Typing | Whole blood, blood, serum, plasma, sputum, cerebrospinal fluid (CSF), urine, sputum, swab, bronchoalveolar lavage fluid (BAL) |
| Shelf Life | 18 months |
| Shipping / Storage | -80°C / -20°C |
Manual Spin Column Kits
Bosphore Nucleic Acid Extraction Versatile Spin Kit has been designed for manual extraction of nucleic acids from various samples. The kit contains the required solutions for extraction as well as the plastic consumables for spin column. Bosphore Nucleic Acid Extraction Versatile Spin Kit is compatible with Bosphore Real-Time PCR kits.
Bosphore Viral DNA Extraction Spin Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Viral DNA Extraction Spin Kit has been designed for manual extraction of viral DNA from body fluids including blood, serum, plasma, sputum, CSF as well as cell culture supernatants. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Viral DNA Extraction Spin Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample volume is 200 µl and DNA recovery (elution) volume is 60 µl.
Bosphore Viral RNA Extraction Spin Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Viral RNA Extraction Spin Kit has been designed for manual extraction of viral RNA from body fluids including blood, serum, plasma, sputum, CSF as well as cell culture supernatants. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Viral RNA Extraction Spin Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample volume is 400 µl and RNA recovery (elution) volume is 60 µl.
Bosphore Viral DNA/RNA Extraction Spin Kit has been designed for manual extraction of viral DNA/RNA from body fluids including blood, serum, plasma, sputum and cerebrospinal fluid. The kit contains the required solutions for extraction as well as the plastic consumables for spin column. Bosphore Viral DNA/RNA Extraction Spin Kit is compatible with Bosphore Real-Time PCR kits. Starting sample volume is 400 µl and RNA recovery (elution) volume is 60 µl.
Bosphore Genomic DNA Extraction Spin Kit v2, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Genomic DNA Extraction Spin Kit v2 has been designed for manual extraction of genomic DNA from blood samples. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Genomic DNA Extraction Spin Kit v2 is highly compatible with Bosphore Real-Time PCR kits. Starting sample volume is 200 µl and DNA recovery (elution) volume is 60 µl. The final genomic DNA concentration is 80 µg/ml and the purity value A260/A280 is between 1.8 and 2.0.
Bosphore Tissue Genomic DNA Extraction Kit has been designed for manual extraction of genomic DNA from human and animal tissue samples, and complex food matrixes such as gelatin or processed food. The kit contains the required solutions for extraction as well as the plastic consumables for magnetic bead based extraction. Bosphore Tissue Genomic DNA Extraction Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample amount is 20-150 mg and DNA recovery (elution) volume is 60-100 µl.
Bosphore Tissue Genomic DNA Extraction Spin Kit v2, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Tissue Genomic DNA Extraction Spin Kit v2 has been designed for manual extraction of genomic DNA from animal tissue samples. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Tissue Genomic DNA Extraction Spin Kit v2 is highly compatible with Bosphore Real-Time PCR kits. Starting sample amount is 25-75 mg and DNA recovery (elution) volume is 50 µl.
Bosphore Bacterial DNA Extraction Spin Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Bacterial DNA Extraction Spin Kit has been designed for manual extraction bacterial DNA from body fluids including blood, serum, urine, sputum, swab, plasma, bronchoalveolar lavage (BAL) fluid, cerebrospinal fluid and as well as cell culture supernatants.
The kit contains the required reagents for extraction as well as plastic consumables for spin column. Bosphore Bacterial DNA Extraction Spin Kit is highly compatible with Bosphore Real-Time PCR kits.
Bosphore Cultured Cell DNA Extraction Spin Kit, is a Real-Time PCR based in-vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore Cultured Cell DNA Extraction Spin Kit has been designed for manual extraction of genomic DNA from mammalian cell cultures. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Cultured Cell DNA Extraction Spin Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample volume is 5×106 cells and DNA recovery (elution) volume is 50 µl.
Bosphore Total RNA Extraction Spin Kit has been designed for manual extraction of total RNA from whole blood or various tissue samples. Genomic DNA are removed with the help On-Column DNase digestion. The kit contains the required solutions for extraction as well as the plastic consumables for spin column. The DNase enzyme is not supplied within the kit.
*For research use only.
Bosphore PCR Product Purification Spin Kit has been designed for purification of double or single stranded DNA products from amplification reactions and DNA cleanup from other enzymatic reactions. The kit contains the required solutions for purification as well as the plastic consumables for spin column.
Bosphore PCR Product Purification Spin Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample amount is 25-75 μl and DNA recovery (elution) volume is 50 μl.
Bosphore Plant Genomic DNA Extraction Spin Kit has been designed for manual extraction of genomic DNA from plant tissue samples. The kit contains the required reagents for extraction as well as plastic consumables for spin column.
Bosphore Plant Genomic DNA Extraction Spin Kit is highly compatible with Bosphore Real-Time PCR kits. Starting sample amount is 20-150 mg and DNA recovery (elution) volume is 60 µl.
*For research use only.