Bosphore Monkeypox Detection Kit v1 has been designed to detect Monkeypox virus in human biological samples including lesion swab, whole blood, serum. Recommended biological specimens for laboratory diagnosis of monkeypox are skin lesion material, including swabs of lesion surface and/or exudate, roofs from multiple lesions, or lesion crusts . Fluorescence detection is accomplished using the FAM and HEX filters. A region within the F3L gene is amplified and fluorescence detection is accomplished using the FAM filter.
An internal control has been integrated into the kit to check nucleic acid extraction, PCR inhibition and application mistakes. The amplification data of the internal control is detected with the HEX filter. The internal control can be added either during DNA extraction or PCR step.
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