Bosphore® Species Identification Kit Horse-Donkey v1 detects horse and donkey DNA in meat, skin, tendon, bone, fat, gelatin, candy and dairy samples. A region within the horse genome is amplified and fluorescence detection is accomplished using HEX filter. A region within the donkey genome is amplified and fluorescence detection is accomplished using Cy5 filter.
An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the FAM filter. The internal control can be added either during DNA extraction or PCR step.
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