SARS-CoV-2/Respiratory Panel v1

Bosphore® SARS-CoV-2/Respiratory Pathogens Panel Kit v1 detects and discriminates 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B , Influenza A&B, Enterovirus, Metapneumovirus, Adenovirus, Human Parainfluenza 1/2/3/4, Rhinovirus, Mycoplasma pneumoniae and Legionella Pneumophila, in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva.

 

Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters. SARS-CoV-2 is detected by two regions of the virus (orf1ab and N gene nCoV specific) from a single channel FAM.  RSV A&B is detected by HEX channel. Influenza A&B is detected by Cy5 channel. Enterovirus is detected by FAM channel.  Metapneumovirus is detected by HEX channel. Adenovirus is detected by Cy5 channel. Human parainfluenza Virus 1/2/3/4 is detected by FAM channel. Rhinovirus is detected by HEX channel. Legionella Pneumophila is detected by Texas Red channel. Mycoplasma pneumoniae is detected by Cy5 channel. 

 

An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. Internal control is detected by Texas Red channel in Tube 1 and Tube 2.

 

 

FAM

HEX

Cy5

Texas RED

Tube 1

SARS-Cov 2

RSV A&B

Influenza A&B

Internal Control

Tube 2

Enterovirus

Metapneumovirus

Adenovirus

Internal Control

Tube 3

Human Parainfluenza 1/2/3/4

Rhinovirus

Mycoplasma pneumoniae

Legionella Pneumophila