Bosphore SARS-CoV-2/Flu/RSV Panel Kit v2 detects and discriminates 2019-nCoV (which is later renamed officially as Severe acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and Influenza A, Influenza B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and oral fluids. Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters.
SARS-CoV-2 is detected by three regions of the virus in a single reaction with Master Mix 1: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. RSV A&B, Influenza A and Influenza B are detected and discriminated with Master Mix 2.
Master Mix 1
Internal control (RnaseP)
Master Mix 2
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