Bosphore® Novel Coronavirus (2019-nCoV) Detection Kit v4 detects and characterizes 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.
Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.
An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
(Human RNase P gene)
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