Bosphore® Novel Coronavirus (2019-nCoV) Detection Kit v3 detects and characterizes 2019-nCoV (which is later renamed officially as Severe acute respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by WHO) in human respiratory samples including nasopharyngeal, oropharyngeal swabs and sputum.
Fluorescence detection is accomplished using FAM, HEX, Texas RED and Cy5 filters. SARS-CoV-2 is detected by three regions of the virus in a single reaction: E gene is used for screening purpose, where SARS-CoV-2 and also the closely related coronaviruses are detected, and the orf1ab target region and N gene region are used to discriminate SARS-CoV-2 specifically.
An internal control has been integrated into the kit in order to check RNA extraction, PCR inhibition and application errors. The internal control can be added either during RNA extraction or PCR step.
In order to tackle the existing global shortage on extraction kits under these extra-ordinary circumstances, we have developed an alternative, fast-extraction, which does not require a separate extraction but only a pre-treatment that takes less than 10 minutes. This method helps the institutions and medical workers save precious time by eliminating the need for extraction for diagnosis. Bosphore EX-Tract Dry Swab RNA Solution has been optimized to perform only with Bosphore Novel Coronavirus (2019-nCoV) Detection Kits.
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