Bosphore® N501Y Detection Kit v1 detects and characterizes A570D, P681H, and Y144del mutations of SARS-CoV-2, which are some of the common variants observed in the UK (Lineage B.1.1.7), in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. Fluorescence detection is accomplished using FAM and HEX filters. Detection of A570D, P681H, and Y144del mutations is achieved in a single reaction. An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors.
Samples that will be tested with this kit must be already tested and found to be positive for SARS-CoV-2 prior to use.
A570D / P681H / Y144del
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