Toxoplasmosis is difficult to diagnose as it can be confused with infectious and non-infectious infections. It can be detected by indirect serological methods based on antibody detection (Sabin-Feldman Dye Test, Lysis Test, Agglutination, Precipitation, Fluorescent Techniques, ELISA (IgG, IgM, IgA, IgE) etc.) or direct methods (PCR, Hybridization, isolation of the parasite, histology etc.).
Commonly used serologic tests can cause false positive results because they can detect antibody levels (IgM) that remain detectable in the body for a long time (1-2 years). In addition, in the process of specific antibodies reaching a detectable level after infection (1-3 weeks) (especially determination of the active period in congenital toxoplasmosis) and in people with immunodeficiency, evaluation in serological diagnostic methods becomes difficult. Tissue culture and isolation methods used as an alternative to these methods are time consuming and not easy and pose a risk to health.
With the Real-Time PCR method, which is based on the detection of T. gondii DNA from body fluids and tissues, all these handicaps are avoided, a fast and reliable solution is offered, and parasite quantification can be realized, which enables the follow-up of treatment.