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Bosphore Coronavirus Panel Kit v1

Bosphore® Coronavirus Panel Kit v1 is, a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Coronavirus Panel Kit v1 detects and characterizes Coronavirus 229E, Coronavirus HKU1, Coronavirus OC43 and Coronavirus NL63 in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

In the first PCR tube with PCR Master Mix 1, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas Red filter, Coronavirus HKU1 genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

 

In the second PCR tube with PCR Master Mix 2, Coronavirus OC43 genome is amplified and fluorescence detection is accomplished using the FAM filter, Coronavirus NL63 genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

An internal control has been integrated into the kit in order to check DNA extraction and PCR inhibition. The amplification data of the internal control is detected with the HEX filter in PCR Master Mix 1, Texas RED filter in PCR Master Mix 2. The internal control can be added either during DNA extraction or PCR step.