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Bosphore Respiratory Pathogens Panel Kit v4

Bosphore® Respiratory Pathogens Panel Kit v4, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Respiratory Pathogens Panel Kit v4 detects and characterizes Influenza B, Mycoplasma pneumoniae, Klebsiella pneumoniae, Parainfluenza 2, Parainfluenza 4, Parainfluenza 1, Metapneumovirus, Enterovirus, Influenza A, Parainfluenza 3, RSV A/B, Bocavirus, Rhinovirus, Coronavirus 229E, Pandemic H1N1 influenza A, Seasonal H1N1 influenza A, Salmonella enterica, Adenovirus, Moraxella catarrhalis, Bordetella pertussis, Haemophilus influenzae type B, Parechovirus, Staphylococcus aureus, Pneumocystis jiroveci, Streptococcus pneumoniae, Legionella pneumophila, Coronavirus OC43 and Coronavirus NL63  in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

  • In the first PCR tube with PCR Master Mix 1, Influenza B genome is amplified and fluorescence detection is accomplished using the HEX filter, Mycoplasma pneumoniae genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Klebsiella pneumoniae genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

  • In the second PCR tube with PCR Master Mix 2, Parainfluenza 2 genome is amplified and fluorescence detection is accomplished using the HEX filter, Parainfluenza 4 genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Parainfluenza 1 genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

  • In the third PCR tube with PCR Master Mix 3, Metapneumovirus genome is amplified and fluorescence detection is accomplished using the HEX filter and Enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

  • In the fourth PCR tube with PCR Master Mix 4, Influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, Parainfluenza 3 genome is amplified and fluorescence detection is accomplished using the Texas RED filter and RSV A/B genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

  • In the fifth PCR tube with PCR Master Mix 5, Bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter, Rhinovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas RED filter. 

 

  • In the sixth PCR tube with PCR Master Mix 6, Pandemic H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, Seasonal H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella enterica genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

 

  • In the seventh PCR tube with Master Mix 7, Adenovirus genome is amplified and fluorescence detection is accomplished using the FAM filter, Moraxella catarrhalis genome is amplified and fluorescence detection is accomplished using the HEX filter and Bordetella pertussis genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

 

  • In the eighth PCR tube with Master Mix 8, Haemophilus influenzae type B is amplified and fluorescence detection is accomplished using the FAM filter, Parechovirus genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

  • In the ninth PCR tube with Master Mix 9, Staphylococcus aureus is amplified and fluorescence detection is accomplished using the HEX filter and Pneumocystis jiroveci genome is amplified and fluorescence detection is accomplished using the Texas RED filter.

 

  • In the tenth PCR tube with Master Mix 10, Streptococcus pneumoniae genome is amplified and fluorescence detection is accomplished using the HEX filter and Legionella pneumophila genome is amplified and fluorescence detection is accomplished using the Texas RED filter.

 

  • In the eleventh PCR tube with Master Mix 11, Coronavirus OC43 is amplified and fluorescence detection is accomplished using the FAM filter, Coronavirus NL63 is amplified and fluorescence detection is accomplished using the HEX filter.

 

An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas RED filter in PCR Master Mix 1, PCR Master Mix 2, PCR Master Mix 3, PCR Master Mix 6, Master Mix 7, Master Mix 8 and PCR Master Mix 11, while it is detected with the Cy5 in PCR Master Mix 4, PCR Master Mix 5, PCR Master Mix 9 and PCR Master Mix 10. The internal control can be added either during DNA extraction or PCR step.