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Bosphore Respiratory Pathogens Panel Kit v1

Bosphore® Respiratory Pathogens Panel Kit v1, is a Real-Time PCR-based in vitro diagnostic medical device, IVD CE marked according to 98/79/EC Directive. Bosphore® Respiratory Pathogens Panel Kit v1 detects and characterizes Influenza B, Mycoplasma pneumoniae, Klebsiella pneumoniae, Parainfluenza 2, Parainfluenza 4, Parainfluenza 1, Metapneumovirus, Enterovirus, Influenza A, Parainfluenza 3, RSV A/B, Bocavirus, Rhinovirus, Coronavirus 229E, Pandemic H1N1 influenza A, Seasonal H1N1 influenza A and Salmonella in human respiratory samples. Fluorescence detection is accomplished using the FAM, HEX, Texas RED and Cy5 filters.

 

In the first PCR tube with PCR Master Mix 1, Influenza B genome is amplified and fluorescence detection is accomplished using the HEX filter, Mycoplasma pneumoniae genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Klebsiella pneumoniae genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

In the second PCR tube with PCR Master Mix 2, Parainfluenza 2 genome is amplified and fluorescence detection is accomplished using the HEX filter, Parainfluenza 4 genome is amplified and fluorescence detection is accomplished using the Cy5 filter and Parainfluenza 1 genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

In the third PCR tube with PCR Master Mix 3, Metapneumovirus genome is amplified and fluorescence detection is accomplished using the HEX filter and Enterovirus genome is amplified and fluorescence detection is accomplished using the FAM filter.

 

In the fourth PCR tube with PCR Master Mix 4, Influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, Parainfluenza 3 genome is amplified and fluorescence detection is accomplished using the Texas RED filter and RSV A/B genome is amplified and fluorescence detection is accomplished using the HEX filter.

 

In the fifth PCR tube with PCR Master Mix 5, Bocavirus genome is amplified and fluorescence detection is accomplished using the FAM filter, Rhinovirus genome is amplified and fluorescence detection is accomplished using the HEX filter, Coronavirus 229E genome is amplified and fluorescence detection is accomplished using the Texas RED filter.

 

In the sixth PCR tube with PCR Master Mix 6, Pandemic H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the FAM filter, Seasonal H1N1 influenza A genome is amplified and fluorescence detection is accomplished using the HEX filter and Salmonella genome is amplified and fluorescence detection is accomplished using the Cy5 filter.

 

An internal control has been integrated into the kit in order to check PCR inhibition. The amplification data of the internal control is detected with the Texas RED filter in PCR Master Mix 1, PCR Master Mix 2, PCR Master Mix 3 and PCR Master Mix 6, it is detected with the Cy5 in PCR Master Mix 4 and PCR Master Mix 5. The internal control can be added either during DNA extraction or PCR step. 

 

 

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